| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Research Abstract |
1) In this study, we examine whether room-temperature ionic liquids (RTILs) as a membrane solution can be utilized for hydrocarbon separation from petroleum, and organic acid and penicillin G from the fermented broth by using supported liquid membrane. We used the hexafluorophosphates of 1-n-butyl-, 1-n-hexyl-and 1-n-octyl-3-methyl imidazolium and quaternary ammonium salts as an ionic liquid. The supported liquid membrane was prepared by impregnating the porous polyvinylidene fluoride film with the ionic liquid. Organic sulfur and nitrogen compounds, peniciliin G and lactic acid were successfully transported through the membrane based on the ionic liquids.
(2) Enantiopure chemicals are important in pharmaceutical and agricultural, because each enantiomer shows different activity for human body. Specially, chiral diols are one of the most excellent compounds as intermediate materials, therefore enantiopure preparation method is required. Ionic liquid is a novel medium for enzymatic reactions and has characteristics such as nonvolatile, inflame ability, thermal stability and high polarity. It has shown high stability and enantioselectivity in enzymatic reaction. In this research, it was performed kinetic resolution of rac-epoxides by epoxide hydrolase, which has enantioselectivity, for preparing enantiopure diol in biphasic system of buffer/ionic liquid. Epoxide hydrolase from Rhodotorula glutinis, which had reported for high enantioselectivity for 1,2-epoxyhexane as substrate, was used for this research. Epoxide hydrolase from Rhodotorula glutinis ATCC201718 shows high catalytic activity without being extracted from whole-cell, and excellent enantioselectivity for R-enantiomer was confirmed.
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