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Development of a culture process for preventing transformation of normal hepatic stem cells and its application to regenerative medicine

Research Project

Project/Area Number 17560693
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionSojo University

Principal Investigator

MATSUSHITA Taku  Sojo University, Dept of Applied Life Science, Professor, 生物生命学部, 教授 (10209538)

Co-Investigator(Kenkyū-buntansha) UEOKA Ryuichi  Sojo University, Dept of Applied Life Science, Professor, 生物生命学部, 教授 (70099076)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordshepatic stem cell / hepatoblast / PIVKA-II / transformation / hybrid liposome / three dimensional culture / apoptosis / regenerative medicine / リボソーム / リポソーム
Research Abstract

For regenerative medicine, it is necessary that stem cells will be proliferated in vitro to be attained a required cell number for the cell therapy. If normal stem cells transform in propagation process, these cells can not be applicable to regenerative medicine for the risk of tumor genesis. In fact, the stem cells and transformed tumor cells have similar characteristics and makers. In this research, we studied on development of new culture process for preventing transformation of human hepatic stem cells (hepatoblasts) using PIVKA-II (abnormal prothrombin) as a transformation maker, which is clinical tumor maker of hepatocytes.
1, Effect of three dimensional culture on the transformation of hepatoblasts
Human hepatoblasts formed the three dimensional spheroid on the poly-L-glutamic acid coated dish. The PIVKA-II production rate of hepatoblasts was decreased and their transformation was prevented.
2, Effect of hybrid liposomes on the induction of apoptosis of transformed hepatoblasts
Hybrid liposomes developed by Prof. Ueoka et al., are known to induce apoptosis of transformed tumor cells, but not of normal cells. In this study, It was suggested that decrease of PIVKA-II production and apoptosis induction of hepatoblasts by the treatment of hybrid liposomes.
3, Effect of culture carriers on the transformation of hepatoblasts
For mass culture of human stem cells, it is necessary to study on material characteristics of culture carriers for preventing transformation of the cells. In this research, we examined several culture carriers. Macro porous carrier was better for cultivating human hepatoblasts at high density and preventing transformation of the cells.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (17 results)

All 2006 2005

All Journal Article (17 results)

  • [Journal Article] Spheroid Formation and Enhancement of Cytochrome P450 Activity for Human Fetal Hepatocytes on Poly-L-glutamic Acid Coated Dish2006

    • Author(s)
      T.Matsushita, A.Kiyota, Y.Nishikura, M.Anno, R.Ueoka
    • Journal Title

      Journal of Oral Tissue Engineering Vol.4,No.1

      Pages: 25-31

    • NAID

      130004456563

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 人工臓器-最近の進歩 「人工肝臓」2006

    • Author(s)
      松下 琢, 清田章文, 上岡龍一
    • Journal Title

      人工臓器 35巻3号

      Pages: 338-340

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] ヒト胎児肝細胞からの肝芽細胞の誘導と形質転換の抑制に関する究2006

    • Author(s)
      清田章文, 小嶋 瞬, 竹下晴久, 松下 琢, 上岡龍一
    • Journal Title

      再生医療 5巻増刊号

      Pages: 37-37

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Spheroid Formation and Enhancement of Cytochrome P450 Activity for Human Fetal Hepatocytes on Poly-L-glutamic Acid Coated Dish2006

    • Author(s)
      T.Matsushita, A.Kiyota, Y.Nishikura, M.Anno, R.Ueoka
    • Journal Title

      Journal of Oral Tissue Engineering Vol.4, No.1

      Pages: 25-31

    • NAID

      130004456563

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Artificial Organ-Recent Progress "Artificial Liver"2006

    • Author(s)
      T.Matsushita, A.Kiyota, R.Ueoka
    • Journal Title

      The Japanese Journal of Artificial Organs Vol.35, No.3

      Pages: 338-340

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Studies on induction of hepatoblasts from human fetal hepatocytes and prevention of their transformation2006

    • Author(s)
      A.Kiyota, S.Kojima, H.Takeshita, T.Matsushita, R.Ueoka
    • Journal Title

      Proceedings of The Japanese Society for Regenerative Medicine, 4^<th> Meeting Regenerative Medicine Vol.5(Suppl.)

      Pages: 163-163

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Spheroid formation and enhancement of cytochrome P450 activity for human fetal hepatocytes on poly-L-glutamic acid coated dish2006

    • Author(s)
      T.Matsushita
    • Journal Title

      Journalof Oral Tissue Engineering 4巻1号

      Pages: 25-31

    • NAID

      130004456563

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 人口臓器-最近の進歩「人口肝臓」2006

    • Author(s)
      松下 琢
    • Journal Title

      人口臓器 35巻3号

      Pages: 338-340

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 肝幹細胞の高密度培養と形質転換の抑制に適した培養担体に関する研究2006

    • Author(s)
      松下 琢
    • Journal Title

      化学工学会第38回秋季大会研究発表講演要旨集

    • NAID

      130004664452

    • Related Report
      2006 Annual Research Report
  • [Journal Article] ヒト肝幹細胞の誘導と形質転換の抑制に関する研究2006

    • Author(s)
      小嶋 瞬
    • Journal Title

      化学工学会鹿児島大会研究発表講演要旨集

      Pages: 74-74

    • Related Report
      2006 Annual Research Report
  • [Journal Article] ヒト胎児肝細胞からの肝芽細胞の誘導と形質転換の抑制に関する研究2006

    • Author(s)
      清田章文
    • Journal Title

      再生医療 5巻・2号

      Pages: 163-163

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Novel mechanism of hybrid liposomes-induced apoptosis in human tumor cells2005

    • Author(s)
      Y.Matsumoto, Y.Iwamoto, T.Matsushita, R.Ueoka
    • Journal Title

      International Journal of Cancer Vol.115

      Pages: 377-382

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Inhibitory Effects of Extracts from Shochu Distillation Remnants on the Growth of Human Stomach Tumor Cells2005

    • Author(s)
      Y.Kadota, S.Hirose, H.Iefuji, T.Matsushita, Y.Matsumoto, R.Ueoka
    • Journal Title

      Journal of Chemical Engineering of Japan Vol.38 No.2

      Pages: 154-157

    • NAID

      130000019403

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Inhibitory Effects of Extracts from Shochu Distillation Remnants on the Growth of Human Stomach Tumor Cells2005

    • Author(s)
      Y.Kadota, S.Hirose, H.Iefuji, T.Matsushita, Y.Matsumoto, R.Ueoka
    • Journal Title

      Journal of Chemical Engineering of Japan Vol.38, No.2

      Pages: 154-157

    • NAID

      130000019403

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Novel mechanism of hybrid liposomes-induced apoptosis in human tumor cells.2005

    • Author(s)
      Y Matsumoto
    • Journal Title

      International Journal of Cancer 115巻

      Pages: 377-382

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Inhibitory effects of extracts from Shochu distillation remnants on the growth of human stomach tumor cells.2005

    • Author(s)
      Y Kadota
    • Journal Title

      Journal of Chemical Engineering of Japan 38巻・2号

      Pages: 154-157

    • Related Report
      2005 Annual Research Report
  • [Journal Article] ヒト肝幹細胞の効率的取得と形質転換を抑制する培養プロセスの構築2005

    • Author(s)
      松下 琢
    • Journal Title

      化学工学会第37回秋季大会研究発表講演要旨集

    • NAID

      130005038515

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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