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Photoacclimation of photosynthetic light-harvesting systems

Research Project

Project/Area Number 17570029
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理・分子
Research InstitutionHokkaido University

Principal Investigator

MINAGAWA Jun  Hokkaido Univ., Inst. of Low Temp. Sci., Asso. Prof., 低温科学研究所, 助教授 (80280725)

Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥2,600,000 (Direct Cost: ¥2,600,000)
Keywordsphotosynthesis / green algae / acclimation / light-harvesting system
Research Abstract

State transition in photosynthesis is a short-term balancing mechanism of energy distribution between photosystem I (PSI) and photosystem II (PSII). When PSII is preferentially excited (State 2), a pool of mobile light-harvesting complex II (LHCII) antenna proteins is thought to migrate from PSII to PSI, but biochemical evidence for a physical association between LHCII proteins and PSI in State 2 is weak. Here, using the green alga Chlamydomonas reinhardtii, which has a high capacity for state transitions, we report the isolation of PSI-LHCI supercomplexes from cells locked into State 1 and State 2. We solubilized the thylakoid membranes with a mild detergent, separated the proteins by sucrose density gradient centrifugation, and subjected gradient fractions to gel filtration chromatography. Three LHCII polypeptides were associated with a PSI-LHCI supercomplex only in State 2; we identified them as two minor monomeric LHCII proteins (CP26 and CP29) and one previously unreported major LHCII protein Type II, or LhcbM5. These three LHCII proteins, in addition to the major trimeric LHCII proteins, were phosphorylated upon transition to State 2. The corresponding phylogenetic tree indicates that among the LHCII proteins associated with PSII, these are most similar to the LHC proteins for PSI (LHCI). Our results are important because CP26,CP29, and LhcbM5, which have been viewed as belonging solely to the PSII complex, are now postulated to shuttle between PSI and PSII during state transitions, thereby acting as docking sites for the trimeric LHCII proteins in both PSI and PSII.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (20 results)

All 2007 2006 2005

All Journal Article (15 results) Book (5 results)

  • [Journal Article] Distinct physiological responses to a high light and low CO_2 environment revealed by fluorescence quenching in photoautotrophically grown Chlamydomonas reinhardtii2007

    • Author(s)
      Iwai, M., Kato, N., Minagawa, J.
    • Journal Title

      Photosynth. Res 84(in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Distinct physiological responses to a high light and low CO_2 environment revealed by fluorescence quenching in photoautotrophically grown Chlamydomonas reinhardtii2007

    • Author(s)
      Iwai, M., Kato, N., Minagawa, J.
    • Journal Title

      Photosynth. Res, 84

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Light-harvesting proteins2007

    • Author(s)
      Minagawa, J.
    • Journal Title

      The Chlamydomonas Source Book 2^<nd> edition

    • NAID

      120001498378

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Distinct physiological responses to a high light and low CO_2 environment revealed by fluorescence quenching in photoautotrophically grown Chlamydomonas reinhardtii2007

    • Author(s)
      Iwai, M., Kato, N., Minagawa, J.
    • Journal Title

      Photosynth. Res 84 (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Identification of the mobile light-harvesting complex II polypeptides for state transitions in Chlamydomonas reinhardtii.2006

    • Author(s)
      Takahashi, H., Iwai, M., Takahashi, Y., Minagawa, J.
    • Journal Title

      Proc. Natl. Acad. Sci. U. S. A. 103

      Pages: 477-482

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Real-Time monitoring of chloroplast gene expression by a luciferase reporter : Evidence for nuclear regulation of chloroplast.2006

    • Author(s)
      Matsuo, T., Onai, K., Okamoto, K., Minagawa, J., Ishiura, M.
    • Journal Title

      Mol. Cell. Biol. 26

      Pages: 863-870

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Identification of the mobile light-harvesting complex II polypeptides for state transitions in Chlamydomonas reinhardtii.2006

    • Author(s)
      Takahashi, H., Iwai, M., Takahashi, Y., Minagawa, J.
    • Journal Title

      Proc.Natl.Acad.Sci.USA. 103

      Pages: 477-482

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Real-Time monitoring of chloroplast gene expression by a luciferase reporter : Evidence for nuclear regulation of chloroplast.2006

    • Author(s)
      Matsuo, T., Onai, K., Okamoto, K., Minagawa, J., Ishiura, M.
    • Journal Title

      Mol.Cell.Biol. 26

      Pages: 863-870

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Chlorophyll fluorescence of photosynthetic organisms2006

    • Author(s)
      Minagawa, J.
    • Journal Title

      Bio/Chemiluminescence and its Applications to Photosynthesis

      Pages: 149-174

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification of the mobile light-harvesting complex II polypeptides for state transitions in Chlamydomonas reinhardtii.2006

    • Author(s)
      Takahashi, H.
    • Journal Title

      Proc.Natl.Acad.Sci.U.S.A. 103

      Pages: 477-482

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Chlorophyll fluorescence of photosynthetic organisms2006

    • Author(s)
      Minagawa, J.
    • Journal Title

      Bio/Chemiluminescence and its Applications to Photosynthesis (なし)

      Pages: 149-174

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Nonphotochemical quenching is induced differently under high-light and low-Ci conditions in Chlamydomonas reinhardtii.2006

    • Author(s)
      Iwai, M.
    • Journal Title

      Photosynthesis : Fundamental Aspects to Global Perspectives (なし)

      Pages: 582-584

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Enhanced photoinhibition in the absence of CP29 in Chlamydomonas reinhardtii.2005

    • Author(s)
      Kanno, A., Minagawa, J.
    • Journal Title

      Photosynthesis : Fundamental Aspects to Global Perspectives

      Pages: 483-484

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nonphotochemical quenching is induced differently under high-light and low-Ci conditions in Chlamydomonas reinhardtii2005

    • Author(s)
      Iwai, M., Minagawa, J.
    • Journal Title

      Photosynthesis : Fundamental Aspects to Global Perspectives

      Pages: 582-584

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Enhanced photoinhibition in the absence of CP29 in Chlamydomonas reinhardtii.2005

    • Author(s)
      Kanno, A.
    • Journal Title

      Photosynthesis : Fundamental Aspects to Global Perspectives (なし)

      Pages: 483-484

    • Related Report
      2005 Annual Research Report
  • [Book] The Chlamydomonas Source Book 2^<nd> edition (Light-harvesting proteins)2007

    • Author(s)
      Minagawa, J.
    • Publisher
      Academic Press(in press)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] The Chlamydomonas Source Book 2^<nd> edition2007

    • Author(s)
      Minagawa, J.
    • Publisher
      Academic Press (in press)
    • Related Report
      2006 Annual Research Report
  • [Book] Bio/Chemiluminescence and its Applications to Photosynthesis (Chlorophyll fluorescence of photosynthetic organisms)2006

    • Author(s)
      Minagawa, J.
    • Publisher
      Research Signpost
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] Photosynthesis : Fundamental Aspects to Global Perspectives (Enhanced photoinhibition in the absence of CP29 in Chlamydomonas reinhardtii.)2005

    • Author(s)
      Kanno, A., Minagawa, J.
    • Publisher
      ACG Publishing
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] Photosynthesis : Fundamental Aspects to Global Perspectives (Nonphotochemical quenching is induced differently under high-light and low-Ci conditions in Chlamydomonas reinhardtii.)2005

    • Author(s)
      Iwai, M., Minagawa, J.
    • Publisher
      ACG Publishing
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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