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Establishment of a reptile model animal and the effect of environmental temperature on the reproduction.

Research Project

Project/Area Number 17570048
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphology/Structure
Research InstitutionThe University of Tokyo

Principal Investigator

PARK Min Kyun  The University of Tokyo, Graduate School of Science, Associate Professor, 大学院・理学系研究科, 助教授 (00228694)

Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥2,700,000 (Direct Cost: ¥2,700,000)
Keywordsreptile / sex determination / sex differentiation / temperature-dependent sex determination / GnRH / steroid hormone / bird / gonad
Research Abstract

Squamata species shows various reproductive phenomena, such as oviparity and viviparity, genotypic sex determination and temperature-dependent sex determination to adapt the environments of their habits. These diversities give us a good key to discuss the evolution of reproductive phenomenon in vertebrate. However, the molecular mechanisms underlying such complex traits are still not generally understood because of lacking the model species in this animal group. The leopard gecko, Eublepharis macularius, is easily tamed, and is easy to breed in the laboratory, unlikely other squamata animals. In this animal, the incubation temperature during embryonic development determines their gonadal sex. From such characters, the leopard gecko was chosen in this study.
To investigate on the effect of environmental factor on the expression of sex-differentiation related gene, we had cloned the cDNAs for P450scc, P450arom, estrogen receptors, and SF-1. And the effect of incubation temperature on the mRNA expression of these genes was examined quantitatively. The sex differences of the expression levels of P450arom and SF-1 were detected after hatching. The mRNA expression of these steroidogenic enzymes was detected not only in the brain but also in the gonad prior to the temperature-sensitive period of sex determination. The mRNAs of the four sex steroid hormone receptors were also detected in the brain and gonads at all stages examined. These results strongly suggest the existence of a gonad-independent sex steroid hormone signaling system in the developing leopard gecko brain.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (46 results)

All 2007 2006 2005 Other

All Journal Article (46 results)

  • [Journal Article] Comparative analysis of the pituitary and ovarian GnRH systems in the leopard gecko : signaling crosstalk between multiple receptor subtypes in ovarian follicles.2007

    • Author(s)
      Ikemoto T, Park MK
    • Journal Title

      Journal of Molecular Endocrinology 38

      Pages: 289-304

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A system for receptor functional analysis based on c-fos mRNA expression : Analysis of GnRH receptors as a test system.2007

    • Author(s)
      Ikemoto T, Park MK
    • Journal Title

      Journal of Biochemical and Biophysical Methods. 70

      Pages: 349-353

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Terminal nerve GnRH neurons express multiple GnRH receptors in ateleost, the dwarf gourami (Colisa lalia).2007

    • Author(s)
      Hajdu P, Ikemoto T, Akazome Y, Park MK, Oka Y
    • Journal Title

      Journal of Neuroendocrinology 19

      Pages: 475-479

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Expression of sex steroid hormone-related genes in the embryo of the leopard gecko.2007

    • Author(s)
      Endo, D, Kanaho, Y-I, Park MK
    • Journal Title

      General and Comparative Endocrinology (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Sex difference in Ad4BP/SF-1 mRNA expression in the chick embryo brain before gonadal sexual differentiation.2007

    • Author(s)
      Endo, D, Murakami, S, Akazome, Y., Park MK
    • Journal Title

      Zoological Science (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Comparative analysis of the pituitary and ovarian GnRH system in the leopard gecko : signaling crosstalk between multiple receptor subtypes in ovarian follicles.2007

    • Author(s)
      Ikemoto T, Park MK.
    • Journal Title

      Journal of Molecular Endocrinology 70

      Pages: 289-304

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A system for receptor functional analysis based on c-fos mRNA expression : Analysis of GnRH receptors as a test system.2007

    • Author(s)
      Ikemoto t, Park MK.
    • Journal Title

      Journal of Biochemical and Biophysical Methods 70

      Pages: 349-353

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Terminal nerve GnRH neurons express multiple GnRH receptors n a teleost, the dwarf gourami (Colisa lalia).2007

    • Author(s)
      Hajdu P, Ikemoto T, Akazome Y, Park MK, Oka Y.
    • Journal Title

      Journal of Neuroendocrinology 19

      Pages: 475-479

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Comparative analysis of the pituitary and ovarian GnRH systems in the leopard gecko : signaling crosstalk between multiple receptor subtypes in ovarian follicles.2007

    • Author(s)
      Ikemoto T, Park MK.
    • Journal Title

      Journal of Molecular Endocrinology 38

      Pages: 289-304

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A system for receptor functional analysis based on c-fosmRNA expression : Analysis of GnRH receptors as a test system.2007

    • Author(s)
      Ikemoto T, Park MK.
    • Journal Title

      Journal of Biochemical and Biophysical Methods. 70

      Pages: 349-353

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Terminal nerve GnRH neurons express multiple GnRH receptors in a teleost, the dwarf gourami (Colisa lalia).2007

    • Author(s)
      Hajdu P, Ikemoto T, Akazome Y, Park MK, Oka Y.
    • Journal Title

      Neroendocrinology (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Expression of sex steroid hormone-related genes in the embryo of the leopard gecko2007

    • Author(s)
      Endo, D, Kanaho, Y-I, Park MK.
    • Journal Title

      General and Comparative Endocrinology (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Identification and molecular characterisation of three GnRH ligands and five GnRH receptors in the spotted green pufferfish.2006

    • Author(s)
      Ikemoto T, Park MK
    • Journal Title

      Molecular and cellular endocrinology 242・ 1-2

      Pages: 67-79

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification of the reptilian prolactin and its receptor cDNAs in the leopard gecko, Eublepharis macularius.2006

    • Author(s)
      Kato K, Ikemoto T, Park MK
    • Journal Title

      Gene 346・1

      Pages: 267-276

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Chicken RFamide-related peptide (GnIH) and two distinct receptor subtypes : Identification, molecular characterization and evolutionary considerations.2006

    • Author(s)
      Ikemoto T, Park MK
    • Journal Title

      The Journal of reproduction and development 51・ 3

      Pages: 359-377

    • NAID

      130000054726

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular cloning of P450 aromatase from the leopard gecko and its expression in the ovary.2006

    • Author(s)
      Endo D, Park MK
    • Journal Title

      The Journal of steroid biochemistry and molecular biology 96・2

      Pages: 131-140

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The mouse ortholog of EFHC1 implicated in juvenile myoclonic epilepsy is an axonemal protein widely conserved among organisms with motile cilia and flagella.2006

    • Author(s)
      Ikeda T, Ikeda K, Enomoto M, Park MK, Hirono M, Kamiya R
    • Journal Title

      FEBS letters 579・3

      Pages: 819-822

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Gonadotropin-releasing hormone induces actin cytoskeleton remodeling and affects cell migration in a cell-type-specific manner in TSU-Prl and DU145 cells.2006

    • Author(s)
      Enomoto M, Utsumi M, Park MK
    • Journal Title

      Endocrinology 147

      Pages: 530-542

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular and evolutionary characterization of the GnRH-II gene in the chicken : distinctive genomic organization, expression pattern, and precursor sequences.2006

    • Author(s)
      Ikemoto T, Park MK
    • Journal Title

      Gene 368

      Pages: 28-36

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Comparative genomics of the endocrine systems in humans and chimpanzees with special reference to GnRH-II and UCN-II and their receptors.2006

    • Author(s)
      Ikemoto T, Park MK
    • Journal Title

      Genomics 87

      Pages: 459-463

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular characterization of thyroid hormone receptors from the leopard gecko and their differential expression in the skin.2006

    • Author(s)
      Kanaho Y-I, Endo D, Park MK
    • Journal Title

      Zoological Science 23

      Pages: 549-556

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The effect of incubation temperature on the expression of p450 aromatase and SOX9 in the gonad of leopard gecko, a lizard with temperature-dependent sex determination.2006

    • Author(s)
      Endo D, Kanaho, Y-I, Park MK
    • Journal Title

      In "Proceedings of the 5^<th> Intercongress Symposium of AOSCE"

      Pages: 95-97

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Comparative analysis of the pituitary and gonadal GnRH systems in vertebrates.2006

    • Author(s)
      Ikemoto T, Park MK
    • Journal Title

      In "Proceedings of the 5^<th> Intercongress Symposium of AOSCE"

      Pages: 181-184

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification and molecular characterization of three GnRH ligands and five GnRH receptors in the spotted green pufferfish.2006

    • Author(s)
      Ikemoto T, Park MK.
    • Journal Title

      Molecular and cellular endocrinology 346

      Pages: 67-79

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification of the reptilian prolactin and its receptor cDNA in the leopard gecko, Eublepharis macularius.2006

    • Author(s)
      Kato K, Ikemoto T, Park MK.
    • Journal Title

      Gene 346

      Pages: 267-276

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Chicken RFamide-related peptide (GnIH) and two distinct receptor subtypes : Identification, molecular characterization and evolutionary considerations.2006

    • Author(s)
      Ikemoto T, Park MK.
    • Journal Title

      The Journal of reproduction and development 51

      Pages: 359-377

    • NAID

      130000054726

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular cloning of P450 aromatase from the leopard gecko and its expression in the ovary.2006

    • Author(s)
      Endo D, Park MK.
    • Journal Title

      The Journal of steroid biochemistry and molecular biology 96

      Pages: 131-140

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The mouse ortholog of EFHC1 implicated in juvenile myoclonic epilepsy is an axonemal protein widely conserved among organisms with motile cilia and flagella.2006

    • Author(s)
      Ikeda T, Ikeda K, Enomoto M, Park MK, Hirono M, Kamiya R.
    • Journal Title

      FEBS letters 579

      Pages: 819-822

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Gonadotropin-releasing hormone induces actin cytoskeleton remodeling and affects cell migration in a cell-type-specific manner in TSU-Pr1 and DU145 cells.2006

    • Author(s)
      Enomoto M, Utsumi M, Park MK.
    • Journal Title

      Endocrinology 147

      Pages: 530-542

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Molecular and evolutionary characterization of the GnRH-II gene in the chicken : distinctive genomic organization, expression pattern, and precursor sequences.2006

    • Author(s)
      IKemoto T, Park MK.
    • Journal Title

      Gene 368

      Pages: 28-36

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Comparative genomic of the endocrine systems in humans and chimpanzees with special reference to GnRH-II and UCN-II and their receptors.2006

    • Author(s)
      Ikemoto T, Park MK.
    • Journal Title

      Genomics 87

      Pages: 459-463

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular characterization of thyroid hormone receptors from the leopard gecko and their differential expression in the skin.2006

    • Author(s)
      Kanaho Y-I, Endo D, Park MK.
    • Journal Title

      Zoological Science 23

      Pages: 549-556

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The effect of incubation temperature on the expression of p450 aromatase and SOX9 in the gonad of leopard gecko, a lizard with temperature-dependent sex determination.2006

    • Author(s)
      Endo D, Kanaho, Y-I, Park MK.
    • Journal Title

      Proceedings of the 5^<th> Intercongress Symposium of AOSCE

      Pages: 95-97

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Comparative analysis of the pituitary and gonadal GnRH systems in vertebrates.2006

    • Author(s)
      Ikemoto T, Park MK.
    • Journal Title

      Proceedings of the 5^<th> Intercongress Synposium of AOSCE

      Pages: 181-184

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular and evolutionary characterization of the GnRH-II gene in the chicken : distinctive genomic organization, expression pattern, and precursor sequences.2006

    • Author(s)
      Ikemoto T, Park MK.
    • Journal Title

      Gene 368

      Pages: 28-36

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Comparative genomics of the endocrine systems in humans and chimpanzees with special reference to GnRH-II and UCN-II and their receptors.2006

    • Author(s)
      Ikemoto T, Park MK.
    • Journal Title

      Genomics 87

      Pages: 459-463

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Moleclar characterization of thyroid hormone receptors from the leopard gecko and their differential expression in the skin.2006

    • Author(s)
      Kanaho Y-I, Endo D, Park MK.
    • Journal Title

      Zoological Science 23

      Pages: 549-556

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The effect of incubation temperature on the expression of p450 aromatase and SOX9 in the gonad of leopard gecko, a lizard with temperature-dependent sex determination.2006

    • Author(s)
      Endo, D, Kanaho, Y-I, Park MK.
    • Journal Title

      In "Proceedings of the 5^<th> Intercongress Symposium of AOSCE"

      Pages: 95-97

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Comparative analysis of the pituitary and gonadal GnRH systems in vertebrates.2006

    • Author(s)
      Ikemoto T, Park MK.
    • Journal Title

      In "Proceedings of the 5^<th> Intercongress Symposium of AOSCE"

      Pages: 181-184

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Identification and molecular characterisation of three GnRH ligands and five GnRH receptors in the spotted green pufferfish.2005

    • Author(s)
      Ikemoto T, Park MK.
    • Journal Title

      Molecular and cellular endocrinology 242・1-2

      Pages: 67-79

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Identification of the reptilian prolactin and its receptor cDNAs in the leopard gecko, Eublepharis macularius.2005

    • Author(s)
      Kato K, Ikemoto T, Park MK.
    • Journal Title

      Gene 346・1

      Pages: 267-276

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Chicken RFamide-related peptide (GnIH) and two distinct receptor subtypes : Identification, molecular characterization and evolutionary considerations.2005

    • Author(s)
      Ikemoto T, Park MK.
    • Journal Title

      The Journal of reproduction and development 51・3

      Pages: 359-377

    • NAID

      130000054726

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Molecular cloning of P450 aromatase from the leopard gecko and its expression in the ovary.2005

    • Author(s)
      Endo D, Park MK.
    • Journal Title

      The Journal of steroid biochemistry and molecular biology 96・2

      Pages: 131-140

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The mouse ortholog of EFHC1 implicated in juvenile myoclonic epilepsy is an axonemal protein widely conserved among organisms with motile cilia and flagella.2005

    • Author(s)
      Ikeda T, Ikeda K, Enomoto M, Park MK, Hirono M, Kamiya R.
    • Journal Title

      FEBS letters 579・3

      Pages: 819-822

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Expression of sex steroid hormone-related genes in the embryo of the leopard gecko.

    • Author(s)
      Endo, D, Kanaho, Y-I, Park MK.
    • Journal Title

      General and Comparative Endocrinology (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Sex difference in Ad4BP/SF-1 mRNA expression in the chick embryo brain before gonadal sexual differentiation.

    • Author(s)
      Endo, D, Murakami, S, Akazome, Y, Park MK.
    • Journal Title

      Zoological Science (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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