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A new approach for functions of classical osmoregulatory hormones in fishes

Research Project

Project/Area Number 17570049
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphology/Structure
Research InstitutionOkayama University

Principal Investigator

SAKAMOTO Tatsuya  Okayama University, Graduate School of Natural Science and Technology, Professor, 大学院環境学研究科, 教授 (10294480)

Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsintestine / osmoregulation / cortisol / apoprosis / cell proliferation / esophagus / medaka / USA: France
Research Abstract

In the seawater (SW)-acclimated euryhaline fishes, the ion/water permeability of the gastrointestinal tract is generally greater than that of freshwater (FW)-acclimated fish. The esophageal epithelium of SW fishes is simple columnar in form, whereas that of FW fishes is stratified. We have previously shown that esophageal epithelium of a euryhaline goby and tilapia displays elevated cell proliferation in FW fish, but undergoes apoptosis during SW acclimation (1). In-vivo cortisol treatment of the goby stimulated not only the apoptosis but also cell proliferation in the esophageal epithelium, whereas 11-deoxycorticosterone, the putative teleostean mineralocorticoid (2), did not have any impact on cell proliferation or apoptosis (3). To understand the possible dual mode of action of cortisol on the esophageal cell turnover, we have developed a method of culture tissue explants of the esophagus from euryhaline medaka (Oryzias latipes). Oligonucleotide detection assay and an oxidation-reduction indicator were used as indicators of apoptosis and cell proliferations, respectively. Addition of cortisol (10 nM) to the culture medium for 8 days stimulated apoptosis in the medaka esophagus, as a well-established glucocorticoid function. No effects were seen at higher doses (100 and 1000 nM). On the other hand, addition of cortisol (1000 nM) for 8 days induced the cell proliferation. The response of cell proliferation was dose-dependent within physiological range (10-1000 nM). Thus, it is likely that cortisol induces directly not only apoptosis but also cell proliferation at the esophagus in euryhaline fishes, possibly via glucocorticoid receptor, since glucocorticoid receptor shows lower sensitivity for cortisol than mineralocorticoid receptor (2).
References
(1) Takahashi et al. (2006). J. Comp. Physiol. B, 176, 463-468.
(2) Prunet et al. (2006). Gen. Comp. Endocrinol., 147, 17-23.
(3) Takahashi et al. (2006). Life Sci., 79, 1873-1880.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (22 results)

All 2007 2006 2005

All Journal Article (20 results) Book (2 results)

  • [Journal Article] Expression of glucocorticoid receptor in the intestine of a euryhaline teleost, the Mozambique tilapia (Oreochromis mossambicus) : effect of seawater exposure and cortisol treatment2006

    • Author(s)
      Takahashi, H.
    • Journal Title

      Life Sci. 78

      Pages: 2329-2335

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Cell proliferation and apoptosis in the anterior intestine of an amphibious, euryhaline mudskipper (Periophthalmus modestus)2006

    • Author(s)
      Takahashi, H.
    • Journal Title

      J. Comp. Physiol. B 176

      Pages: 463-468

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Prolactin-releasing peptide is essential to maintain the prolactin level and osmotic balance in fresh-water teleost fish2006

    • Author(s)
      Fujimoto, M.
    • Journal Title

      Peptides 27

      Pages: 1104-1109

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Prolactin and growth hormone in fish osmoregulation2006

    • Author(s)
      Sakamoto, T.
    • Journal Title

      Gen. Comp. Endocrinol. 147

      Pages: 24-30

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] In vivo effects of thyroid hormone, corticosteroids and prolactin on cell proliferation and apoptosis in the anterior intestine of the euryhaline mudskipper (Periophthalmus modestus)2006

    • Author(s)
      Takahashi, H.
    • Journal Title

      Life Sci. 79

      Pages: 1873-1880

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Molecular cloning and functional characterization of a Prolactin-releasing peptide homolog from Xenopus laevis2006

    • Author(s)
      Sakamoto, T.
    • Journal Title

      Peptides 27

      Pages: 3347-3351

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular cloning and functional characterization of a prolactin-releasing peptide homolog from Xenopus laevis.2006

    • Author(s)
      Sakamoto T., A.Oda, K.Yamamoto, M.Kaneko, S.Kikuyama, A.Nishikawa, A.Takahashi, H.Kawauchi, K.Tsutsui, M.Fujimoto
    • Journal Title

      Peptides 27

      Pages: 3347-3351

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] In vivo effects of thyroid hormone, corticosteroids and prolactin on cell proliferation and apoptosis in the anterior intestine of the euryhaline mudskipper (Periophthalmus modestus).2006

    • Author(s)
      Takahashi H., A.Takahashi, T.Sakamoto.
    • Journal Title

      Life Sci. 79

      Pages: 1873-1880

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Prolactin and growth hormone in fish osmoregulation.2006

    • Author(s)
      Sakamoto T., S.D.McCormick.
    • Journal Title

      Gen. Comp. Endocrinol. 147

      Pages: 24-30

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Prolactin-releasing peptide is essential to maintain the prolactin level and osmotic balance in freshwater teleost fish.2006

    • Author(s)
      Fujimoto M., T.Sakamoto, T.Kanetoh, M.Osaka S., Moriyama, S.
    • Journal Title

      Peptides 27

      Pages: 1104-1109

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cell proliferation and apoptosis in the anterior intestine of an amphibious, euryhaline mudskipper (Periophthalmus modestus)..2006

    • Author(s)
      Takahashi H., T.Sakamoto, K.Narita.
    • Journal Title

      J. Comp. Physiol. B, 176

      Pages: 463-468

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Expression of glucocorticoid receptor in the intestine of a euryhaline teleost, the Mozambique tilapia (Oreochromis mossambicus) : effect of seawater exposure and cortisol treatment.2006

    • Author(s)
      Takahashi H., T.Sakamoto, S.Hyodo, B.S.Shepherd, T.Kaneko, E.G.Grau.
    • Journal Title

      Life Sci. 78

      Pages: 2329-2335

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular cloning and functional characterization of a prolactin-releasing peptide homolog from Xenopus laevis2006

    • Author(s)
      Sakamoto, T.
    • Journal Title

      Peptides 27

      Pages: 3347-3351

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Prolactin and growth hormone in fish osmoregulation2006

    • Author(s)
      Sakamoto, T.
    • Journal Title

      Gen.Comp.Endocrinol. On line first

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Prolactin-releasing peptide is essential to maintain the prolactin level and osmotic balance in fresh-water teleost fish2006

    • Author(s)
      Fujimoto, M.
    • Journal Title

      Peptides On line first

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Cell proliferation and apoptosis in anterior intestine of an amphibious, euryhaline mudskipper (Periophthalmus modestus)2006

    • Author(s)
      Takahashi, H.
    • Journal Title

      J.Comp.Physiol.B On line first

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Expression of glucocorticoid receptor in the intestine of a euryhaline teleost, the Mozambique tilapia (Oreochromis mossambicus) : effect of seawater exposure and cortisol treatment2006

    • Author(s)
      Takahashi, H
    • Journal Title

      Lift Sci. 78

      Pages: 2329-2335

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Expression of prolactin-releasing peptide and prolactin in the euryhaline mudskippers (Periophthalmus modestus) : prolactin-releasing peptide as a primary regulator of prolactin.2005

    • Author(s)
      Sakamoto T., M Amano, S Hyodo, S Moriyama, A Takahashi, H Kawauchi, M Ando
    • Journal Title

      J. Mol. Endocrinol. 34

      Pages: 825-834

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Expression of prolactin-releasing peptide and prolactin in the euryhaline mudskippers (Periophthalmus modestus) : prolactin-releasing peptide as a primary regulator of prolactin.2005

    • Author(s)
      Sakamoto, T.
    • Journal Title

      J.Mol.Endocrinal. 32

      Pages: 825-834

    • Related Report
      2005 Annual Research Report
  • [Journal Article] High ambient ammonia promotes growth in a ureogenic goby, Mugilogobius abei2005

    • Author(s)
      Iwata, K.
    • Journal Title

      J.Comp.Physiol. 175

      Pages: 395-404

    • Related Report
      2005 Annual Research Report
  • [Book] 新・ホルモンハンドブック「成長ホルモン/プロラクチン/ソマトラクチン」2007

    • Author(s)
      坂本竜哉
    • Publisher
      南江堂(予定(確約あり))
    • Related Report
      2006 Annual Research Report
  • [Book] Trends in Comparative Endocrinology and Neurobiology2005

    • Author(s)
      Sakamoto, T
    • Total Pages
      529
    • Publisher
      NY Acad. Sci.
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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