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Expression and distribution of novel peptidehormones in memory and learning system of cephalopod brain

Research Project

Project/Area Number 17570055
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphology/Structure
Research InstitutionSuntory Institute for Bioorganic Research

Principal Investigator

MINAKATA Hiroyuki  Suntory Institute for Bioorganic Research, Researcher, Researcher, 主席研究員 (90150143)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Toshio  Suntory Institute for Bioorganic Research, Researcher, 研究員 (20390792)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsOctopus vulgaris / Gonadotropin-releasing hormone / G-protein-coupled receptor / oxytocin / vasopressin family / expression and distribution / immunohistochemistry / Octpus vulgalis / バソプレシンファミリー / 性腺刺激ホルモン放出ホルモン / クローニング / アフリカツメガエル卵母細胞発現 / in situ hybridization
Research Abstract

An orphan receptor homologous to gonadotropin-releasing peptide (GnRH) was cloned from the brain of octopus (Octopus vulgaris). The receptor expressed in Xenopus oocyte caused Cl^--current by application of oct-GnRH, however, vertebrate GnRHs failed to trigger the current. Using this assay system, the octopus brain extract was fractionated by HPLC, then, the purified material was identified to oct-GnRH. Thus, the cloned receptor was deorphaned to be the oct-GnRH receptor (oct-GnRHR). Oct-GnRHR mRNA was widely expressed in the central and peripheral nervous system, the tissues such as buccal muscle, salivary gland, crop, rectum, aorta, heart, ovary, testis, etc. Oct-GnRH caused contractions of buccal muscle and exhibited the synthesis and release of sex steroids from the ovary and testis. These results suggested that oct-GnRH is a multifunctional peptide with important central roles in neurotransmission and neuromodulation in addition to the peripheral role as a hormone.
The distribution of oct-GnRH in the brain was further studied. Oct-GnRH-immunoreactive neurons and fibers were distributed in the anterior subesophageal mass (movement of arms), posterior subesophageal mass (visceral movement and siphon movement), optic tract lobe and basal lobe (posture control), olfactory lobe, superior buccal lobe (higher center of taste and touch), inferior frontal lobe (higher center of touch), superior frontal lobe (higher center of vision), and vertical lobe (higher center of vision and touch). These higher centers are believed to be homologous to hippocampus and cerebral cortex. Octopressin, an octopus peptide in the oxytocin/vasopressin-superfamily, was also immunoreactive in the vertical lobe. It can be proposed that these peptides have important roles, including feeding, memory, learning, movement, and autonomic functions.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (23 results)

All 2007 2006 2005 Other

All Journal Article (15 results) Book (8 results)

  • [Journal Article] オキシトシン/バソプレッシンスーパーファミリーの進化2006

    • Author(s)
      南方宏之
    • Journal Title

      生体の科学 57・5

      Pages: 426-427

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Structures and diverse functions of frog growth hormone-releasing peptide (fGRP) and its related peptides (fGRP-RPs) : a review2006

    • Author(s)
      Ukena, K.
    • Journal Title

      Journal of Experimental Zoology 305A・9

      Pages: 815-821

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Novel excitatory neuropeptides isolated from a prosobranch gastropod, Thais clavigera : the molluscan counterpart of the annelidan GGNG-peptides2006

    • Author(s)
      Morishita, F
    • Journal Title

      Peptides 27・3

      Pages: 483-492

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Molecular and functional characterization of a novel GnRH receptor isolated from Octopus vulgaris2006

    • Author(s)
      Kanda, A.
    • Journal Title

      Biochemical Journal 395・1

      Pages: 125-135

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Isolation and characterization of a novel small cardioactive peptide-related peptide from the brain of Octopus vulgaris2006

    • Author(s)
      Kanda, A.
    • Journal Title

      Peptides 27・7

      Pages: 1755-1761

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular evolution of oxytocin/vasopressin-superfamily peptide2006

    • Author(s)
      H.Minakata
    • Journal Title

      SEITAI NO KAGAKU 57(5)

      Pages: 426-427

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Structures and diverse functions of frog growth hormone-releasing peptide (fGRP) and its related peptides (fGRP-RPs) : a review2006

    • Author(s)
      K.Ukena
    • Journal Title

      Journal of Experimental Zoology 305A(9)

      Pages: 815-821

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Novel excitatory neuropeptides isolated from a prosobranch gastropod, Thais clavigera : the molluscan counterpart of the annelidan GGNG-peptides2006

    • Author(s)
      F.Morishita
    • Journal Title

      Peptides 27(3)

      Pages: 483-492

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular and functional characterization of a novel GnRH receptor isolated from Octopus vulgaris2006

    • Author(s)
      A.Kanda
    • Journal Title

      Biochemical Journal 395(1)

      Pages: 125-135

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Structures and diverse functions of frog growth hormone-releasing peptide (fGRP) and its related peptides (fGRP-RPs) : areview2006

    • Author(s)
      Ukena, K.
    • Journal Title

      Journal of Experimental Zoology 305A・9

      Pages: 815-821

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Molecular and functional characterization of a novel GnRH receptor isolated from Octopus vulgaris.2006

    • Author(s)
      A.Kanda
    • Journal Title

      Biochemical Journal 395・1

      Pages: 125-135

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Isolation and characterization of a novel small cardioactive peptide-related peptide from the brain of Octopus vulgaris.2006

    • Author(s)
      A.Kanda
    • Journal Title

      Peptides (印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 頭足類生殖に関与する内分泌ホルモン2005

    • Author(s)
      南方宏之
    • Journal Title

      日本比較内分泌学会ニュース 119号

      Pages: 12-16

    • NAID

      10016723686

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Endorine hormones in cephalopod reproduction2005

    • Author(s)
      H.Minakata
    • Journal Title

      Newsletter of Japan Society for Comparative Endocrinology 119

      Pages: 12-16

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Isolation and characterization of a novel small cardioactive peptide-related peptide from the brain of Octopus vulgaris

    • Author(s)
      A.Kanda
    • Journal Title

      Peptides 27(7)

      Pages: 1755-1761

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] ホルモンハンドブック2007

    • Author(s)
      南方宏之
    • Publisher
      南江堂(印刷中)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Book] Invertebrate neuropeptides and hormones : basic knowledge and recent advances2006

    • Author(s)
      Minakata, H
    • Total Pages
      17
    • Publisher
      Transworld Research Network, India
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] Neuropeptides and peptide hormones in cephalopods, Invertebrate neuropeptides and hormones : basic knowledge and recent advances2006

    • Author(s)
      H.Minakata
    • Publisher
      Transworld Research Network, India
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] Invertebrate neuropeptides and hormones : basic knowledge an drecent advances2006

    • Author(s)
      Minakata, H
    • Total Pages
      276
    • Publisher
      Transworld Research Network, India
    • Related Report
      2006 Annual Research Report
  • [Book] Peptide Science 20042005

    • Author(s)
      Iwakoshi-Ukena, E.
    • Total Pages
      4
    • Publisher
      The Japanese Peptide Society
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] Occurrence of Octopus GnRH in the central nervous system and peripheral organs of the octopus (Octopus vulgaris), Peptide Science 20042005

    • Author(s)
      E.Iwakoshi-Ukena
    • Publisher
      The Japanese Peptide Society
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] Peptide Science 20042005

    • Author(s)
      E.Iwakoshi-Ukena
    • Total Pages
      4
    • Publisher
      The Japanese Peptide Society
    • Related Report
      2005 Annual Research Report
  • [Book] Hormones in mollusks, Hormone Handbook

    • Author(s)
      H.Minakata
    • Publisher
      Nankodo, Tokyo(in press)
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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