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Genetic differentiation of genus Pungitius relic to the Japan sea slope of Asiancontinent and Japan archipelago

Research Project

Project/Area Number 17570075
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biodiversity/Systematics
Research InstitutionShinshu University

Principal Investigator

TAKATA Keisuke  Shinshu University, Faculty of Science, Associate Professor (90197096)

Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥2,810,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsPunritiui / relic / allozvme / mtDNA
Research Abstract

Phylogenetic relationship among three relic species of genus Pungnius along the Japan Sea slope both Asian Continent (P. kaibarae and P. bussei) and Japan Achipelago (Omono type of P. pungitius) was assessed by allozyme and mtDNA sequence analyses. Two major genetic groups were detected by 15 loci of enzymes. One genetic group was consist of two populations of P. bussei in the Maritime of Russia and one of the two P. kaibarae populations in middle part of Korean Peninsula face to Japan Sea. Another genetic group contained two populations of the Omono type of P. pungitius in Yamagata and Akita, and one of the P. kaibarae populations face to the Korea Strait.
Partial sequences of CR (346 bp), Cyt-b (872 bp) and ATPase6 (383 bp) of mtDNA were determined for the three species and the freshwater type of P. pungitius. Three major haplotype groups were detected by each of the three genes. Two populations of P. bussei in Russia and P. kaibarae in Korea, respectively, formed a common major haplotype group. Haplotypes of the Omono type in Yamagata were contained to the second major haplotype group. Haplotype of the Omono type in Akita did not belong the same major haplotype group as that of Yamagata but clustered as the third major haplotype group with the freshwater tvoe of P. punritius in Honshu and Hokkaido.
The first major haplotype group containing P. bussei and P. kaibarae may belong to the same haplotype group B proposed by (Takahashi and Goto, 2001), judging from the topology of the NJ tree and sampling locality. Disagreement between phylogenetic trees by allozyme data or mtDNA sequences may result of secondary contacts and consequent introgression of mtDNA from the freshwater type of P. pungitius to the Omono type in Akita.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (9 results)

All 2008 2007 2006 2005

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (4 results) Book (2 results)

  • [Journal Article] Isolation and characterization of polymorphic microsatellite DNA markers in the Omono type of ninespine stickleback, genus Pungitius2007

    • Author(s)
      Koizumi, N
    • Journal Title

      Molecular Ecology Notes 7

      Pages: 1315-1318

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of polymorphic microsatellite DNA markers in the Omono type of ninespinc stickleback, genus Pungilius2007

    • Author(s)
      Koizumi, N. H. Jinguji, H. Takahashi. M. Higuchi, K. Takata, M. Minezawa, T. Takemura, A. Mori
    • Journal Title

      Molecular Ecology Notes7

      Pages: 1315-1318

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Isolation and characterization of polymorphic microsatellite DNA markers in the Omono type of ninespine stickleback, genus Pungitius2007

    • Author(s)
      Koizumi, N., H. Jinguji, H. Takahashi, M. Higuchi, K. Takata, M. Minezawa, T. Takemura & A. Mori
    • Journal Title

      Molecular Ecology Notes 7

      Pages: 1315-1318

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 山形県における絶滅危惧種イバラトミヨ雄物型の生息環境と営巣特性2008

    • Author(s)
      伊藤 聡
    • Organizer
      日本生態学会
    • Place of Presentation
      第55回日本生態学会福岡大会
    • Year and Date
      2008-03-17
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Habitat and spawning site of an endangered ninespine stickleback Pungitins sp. Omono type in Yamagata2008

    • Author(s)
      Itoh, S., K., Watanabe, E., Watanabe, K., Satoh, M., Kawauchi, A., Ohi, K., Takata, S., Maruyoshi
    • Organizer
      Annual meeting of Ecological Society of Japan
    • Place of Presentation
      Fukuoka International Congress Center
    • Year and Date
      2008-03-17
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 絶滅危惧種イバラトミヨ雄物型の山形県小見川における成長様式および生息個体数の推定2007

    • Author(s)
      河内正行
    • Organizer
      日本水産学会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2007-03-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Estimation of growth and population size of an endangered ninespine stickleback Pungitius sp. Omono type in Yamagata2007

    • Author(s)
      Kawauchi, M. A. Ohi, K. Watanabe, S. Itoh, K. Satoh, K. Takata, I. Akoshima
    • Organizer
      Annual meeting of the Japanese Society of Fisheries Science
    • Place of Presentation
      Tokyo University of Marine Science and Technology
    • Year and Date
      2007-03-29
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] ツナイモツゴの保全への模索 -長野県のシナイモツゴを例に- ブラックバスを退治する. (細谷, 高橋編)2006

    • Author(s)
      高田啓介
    • Total Pages
      152
    • Publisher
      恒星社恒星閣
    • Related Report
      2006 Annual Research Report
  • [Book] シナイモツゴからモツゴへ,交雑をとおした種の置き換わり.希少淡水魚の現在と未来 -積極的保全のシナリオ(片野修・森誠一編)2005

    • Author(s)
      小西繭, 高田啓介
    • Total Pages
      416
    • Publisher
      信山社
    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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