| Project/Area Number |
17570088
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | National Museum of Nature and Science, Tokyo |
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Principal Investigator |
HOSOYA Tsuyoshi National Museum of Nature and Science, Tokyo, National Museum of Nature Science, Department of Botany, Senior curator (60392536)
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| Co-Investigator(Kenkyū-buntansha) |
TOKUMASU Seiji Univerisity of Tsukuba, Sugadaira Montane Research Center, Graduate School of Life and Environmental Sciences, Professor (80092526)
OSONO Takashi Kyoto University, Graduate School of Agriculture, Assistant Professor (90335307)
KUBONO Takanori Forestry and Forest Product Research Institute, Department of Forest Microbiology, Chief of Forest Pathology (80353671)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Fagus crenata / Dasyscyphella longistipitata / geographical isolation / genetic diversity / ITS-5.8S ribosomal DNA gene / haplotype / ascomycete / distribution / UPGMA |
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| Research Abstract |
To search for major factors that affected fungal distribution, ITS sequence diversity of Dasyscyphella longistipitata(Hyaloscyphaceae, Helotiales, Disomyoate, Ascomycota) that occur on Fagus crenata cupulus obtained from wide areas in Japan were compared. Five to 20 cupules were collected every 5m apart at each sampling site, and single-spored isolates were obtained. In 120 isolates obtained from 12 sites, 38 haplotypes were detected, and the haplotype H12(43% of the total isolates) was detected from all the populations. In addition, H28(15%) and H10(10%) consisted of the three major haplotypes. These three haplotypes were placed in inner clades in the haplotype network based on statistical parsimony. Nested clade analysis did not indicate geographic isolation. Analysis of molecular variance attributed the genetic diversity for the diversity within the population. Genetic distance was not found to be correlated with geological distance, and no relationship was found with the phylogenetic grouping of the host. These facts suggested the whole the populations of D.longistipitata Japan form a large continuum. However, genetic diversity was higher in south-western areas and north eastern areas in Japan. This genetic distribution pattern suggests that the current population initiated from the ancestral population in the glacier period with high genetic diversity in south-western Japan, followed by the continuous expansion along with the host expansion, decrease of the genetic diversity in the middle Japan due to the extinction of the host, while keeping diversity due to continuous distribution of the host in the north-east Japan.
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