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Elucidation of antigen recognition mechanism by antibody and conformational change of antibody upon antigen binding in correlation with protein fluctuation in solution

Research Project

Project/Area Number 17570096
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionKyoto Prefectural University

Principal Investigator

ODA Masayuki  Kyoto Prefectural University, 大学院・生命環境科学研究科, 准教授 (20318231)

Project Period (FY) 2005 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,960,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywords生体分子 / 蛋白質 / 分析化学 / 免疫学
Research Abstract

いくつかの抗原抗体間相互作用系を対象として、溶液中での蛋白質の動的挙動が反映される熱力学量や速度論量を主な指標として、静的な構造情報を補完する一般側の導出や方法論有効性の検証を目指した。抗体の抗原認識は多種多様であり、その解析から分子認識の一般側が得られ、さらに複数の抗原結合部位の可動性を解析することで、抗原上の比較的小さな構造変化から大きな構造変化にいたる動的挙動に関する結果が得られた。

Report

(5 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (14 results)

All 2008 2007 2006 Other

All Journal Article (4 results) (of which Peer Reviewed: 3 results) Presentation (8 results) Remarks (2 results)

  • [Journal Article] Exploring the energy landscape of antibody-antigen complexes : Protein dynamics, flexibility, and molecular recognition2008

    • Author(s)
      Thielges, M.C., Zimmermann, J., Yu, W., Oda, M., and Romesberg, F.E.
    • Journal Title

      Biochemistry 47

      Pages: 7237-7247

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regional and segmental flexibility of antibodies in the interaction with antigens of different size2006

    • Author(s)
      Oda, M., Uchiyama, S., Robinson, C.V., Fukui, K., Kobayashi, Y., and Azuma, T.
    • Journal Title

      FEBS J 273

      Pages: 1476-1487

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regional and segmental flexibillity of antibodies in the increaction with antigens of different size.2006

    • Author(s)
      Oda, M, Uchiyama, S, Robinson, C.V, Fukui, K, Kobayashi, Y, Azuma,T
    • Journal Title

      FEBS J. 273・7

      Pages: 1476-1487

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Contribution of the trifluoroacetyl group in the thermodynamics of antigen-antibody binding

    • Author(s)
      Oda, M., Saito, M., Tsumuraya, T, and Fujii, I.
    • Journal Title

      J. Mol. Recognit (in press)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Presentation] 触媒抗体7C8と6D9の基質認識の比較2008

    • Author(s)
      織田昌幸
    • Organizer
      第44回熱測定討論会
    • Place of Presentation
      つくば
    • Year and Date
      2008-10-17
    • Related Report
      2008 Final Research Report
  • [Presentation] 触媒抗体7C8と6D9の基質認識の比較2008

    • Author(s)
      織田 昌幸, 円谷 健, 斎藤 稔, 藤井 郁雄
    • Organizer
      第44回 熱測定討論会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2008-10-17
    • Related Report
      2008 Annual Research Report
  • [Presentation] Direct observation of antigen-antibody complex : Effects of antigen size, valence, and binding affinity of antibody2007

    • Author(s)
      Maiko Koga
    • Organizer
      第45回日本生物物理学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-22
    • Related Report
      2008 Final Research Report
  • [Presentation] Direct observation of antigen-antibody complex: Effects of antigen size, valence, and binding affinity of antibody2007

    • Author(s)
      Maiko Koga, Kouta Mayanagi, Aki Kitai, Akikazu Murakami, Takachika Azuma, Masayuki Oda
    • Organizer
      第45回 日本生物物理学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] Molecular basis of antigen-antibody interaction critical for B-cell activation2007

    • Author(s)
      Aki Kitai
    • Organizer
      第37回日本免疫学会総会
    • Place of Presentation
      東京
    • Year and Date
      2007-11-20
    • Related Report
      2008 Final Research Report
  • [Presentation] Molecular basis of antigen-antibody interaction critical for B-cell activation2007

    • Author(s)
      Aki Kitai, Maiko Koga, Akikazu Murakami, Takachika Azuma, Masayuki Oda
    • Organizer
      第37回 日本免疫学会総会
    • Place of Presentation
      東京
    • Year and Date
      2007-11-20
    • Related Report
      2007 Annual Research Report
  • [Presentation] Interactions of antibodies with antigens of different sizes and valences2006

    • Author(s)
      Maiko Koga
    • Organizer
      第36回日本免疫学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2006-12-12
    • Related Report
      2008 Final Research Report
  • [Presentation] 抗リゾチーム抗体の抗原認識能とエピトープ解析2006

    • Author(s)
      北井麻希
    • Organizer
      日本生物高分子学会2006年度大会
    • Place of Presentation
      京都
    • Year and Date
      2006-09-22
    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://www2.kpu.ac.jp/life_environ/biophys_chem/index.html

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://cocktail.kpu.ac.jp/agricul/bmc/oda.html

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

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