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Study on cooperative regulation of actin cytoskeletal organization and translation initiation.

Research Project

Project/Area Number 17570140
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionThe University of Tokyo

Principal Investigator

UESONO Yukifumi  The University of Tokyo, Graduate School of Science, Biological Sciences, Research associate (30251408)

Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsosmotic stress / glucose deprivation / actin / protein synthesis / local anesthetic / antipsychotic drug / 浸透圧 / グルコース / 翻訳 / 界面活性剤 / ストレス / 酵母
Research Abstract

High osmolarity or glucose deprivation causes rapid shutdowns of both actin polarization and translation initiation in yeast. As like stresses, administration of antipsychotic phenothiazines or local anesthetics causes similar responses. All these drugs have amphiphilic structures, and are able to form emulsions and permeabilize the cell membrane, indicating that the drugs have the same features as a surfactant. Consistent with this notion, surfactants induced responses similar to those of antipsychotic drugs and local anesthetics. Benzethonium chloride, a cationic surfactant, has a more potent shutdown activity than phenothiazines, whereas SDS, an anionic surfactant, transiently depolarized actin without inhibiting translation initiation, indicating that a cationic charge in the amphiphile is important for cellular shutdown. The clinical drugs and the cationic surfactants at low concentrations caused shutdown without membrane permeabilization, suggesting that these compounds and the stresses activate the shutdowns, via perturbation rather than disruption of the cell membrane.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (6 results)

All 2005

All Journal Article (4 results) Book (2 results)

  • [Journal Article] LAS24/KOG1, a component of the TOR complex 1(TORC1), is needed for resistance to local anesthetic tetracaine and normal distribution of actin cytoskeleton in yeast2005

    • Author(s)
      Araki T., Uesono Y., Oguchi T., Toh-e A.
    • Journal Title

      Genes Genet. Syst. 80

      Pages: 325-343

    • NAID

      130000062171

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Toh-e A., LAS24/KOG1, a component of the TOR complex l(TORCl), is needed for resistance to local anesthetic tetracaine and normal distribution of actin cytoskeleton in yeast2005

    • Author(s)
      Araki T., Uesono Y., Oguchi T.
    • Journal Title

      Genes Genet. Syst. 80

      Pages: 325-343

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Stability of mRNA2005

    • Author(s)
      Yukifumi Uesono
    • Journal Title

      Handbook of Biotechnology, (Corona publishing co., ltd.)

      Pages: 134-136

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] LAS24/KOG1, a component of the TOR complex 1(TORC1), is needed for resistance to local anesthetic tetracaine and normal distribution of actin cytoskeleton in yeast2005

    • Author(s)
      Araki T., Uesono Y., Oguchi T., Toh-e A
    • Journal Title

      Genes Genet.Syst. 80

      Pages: 325-343

    • NAID

      130000062171

    • Related Report
      2005 Annual Research Report
  • [Book] 生物工学ハンドブック2章5節4項“mRNAの安定性"2005

    • Author(s)
      上園幸史
    • Total Pages
      3
    • Publisher
      コロナ社 (日本生物工学会編)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 生物工学ハンドブック 2章5節4項(日本生物工学会編)2005

    • Author(s)
      上園幸史
    • Total Pages
      3
    • Publisher
      コロナ社
    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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