| Project/Area Number |
17570145
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Nagasaki University |
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Principal Investigator |
NAKAGAWA Takeya Nagasaki University, school of medicine, assistant, 大学院医歯薬学総合研究科, 助手 (50363502)
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| Co-Investigator(Kenkyū-buntansha) |
YASUI Kiyoshi Nagasaki University, school of medicine, assistant, 大学院医歯薬学総合研究科, 助手 (50372777)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | gene / genome / expression control / リン酸化 / クロマチン / ヒストン / 脱リン酸化 |
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| Research Abstract |
We tried to identify the phosphatase of histone H2A c-terminal and clear the effect of this phospholilation for chromatin structure and cell proliferation. But we could not achieve these purposes. We purified the phophatse activity from cell extract and get some candidate molecules. We made recombinant protein of candidate molecules. But these recombinant proteins had no phophatase activity. Next We made the antibody of candidate molecules and used for western blotting. But the fraction which has phsphatase activity contain no candidate molecules. Hereafter we try to analyze another candidate molecules on same ways and purify the phosphatase from the extract which made another way. During this study, we detected the unique histone acetylase activity. So we will try to identify the this acetylase.
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