Spatiotemporal regulation of Mst1 by RAPL is critical for lymphocyte polarity and adhesion
| Project/Area Number |
17570159
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Cell biology
|
|---|
| Research Institution | Kansai Medical University |
|---|
Principal Investigator |
KATAGIRI Koko Kansai Medical University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (00322157)
|
|---|
| Project Period (FY) |
2005 – 2006
|
| Project Status |
Completed (Fiscal Year 2006)
|
| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
|
|---|
| Keywords | Integrin / cell adhesion / cell migration / cell polarity / RAPL / Rap1 / STK / TCR / chemokine / LFA-1 / ICAM-1 / activation |
|---|
| Research Abstract |
RAPL, a Rap1-binding protein is required for efficient immune cell trafficking. Here, we identify mammalian Ste20-like kinase Mst1 as a critical effector of RAPL. RAPL regulates the localization and kinase activity of Mst1. MST1 knockdown demonstrates its requirement for the induction of both a polarized morphology and LFA-1 clustering and adhesion triggered by chemokines and TCR ligation. RAPL and Mst1 localized to vesicular compartments and dynamically translocated with LFA-1 to the leading edge by Rap1 activation, suggesting the regulatory role of RAPL-Mst1 complex in intracellular transport of LFA-1. Our study reveals a novel function for Mst1, relaying the Rap1-RAPL signal to produce the cell polarity and adhesion of lymphocytes.
|
Report
(3 results)
Research Products
(11 results)
-
-
[Journal Article] The M-Ras-RA-GEF-2Rap 1 pathway mediates tumor-necrosis factor-alpha-dependent regulation of integrin activation in splenocytes.2007
Author(s)
Yoshikawa, Y, Satoh, T., Tamura, T., Wei, P., Bilasy, SE., Edamatsu, H., Aiba, A, Katagiri, K., Kinashi, T., Nakao, K., Kataoka, T.
-
Journal Title
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
-
-
-
-
-
[Journal Article] CXCL13 is an arrest chemokine for B cells in high endothelial venules.2005
Author(s)
Kanemitsu, N., Ebisuno, Y., Tanaka, T., Otani, K., Hayasaka, H., Kaisho, T., Akira, S., Katagiri, K., Kinashi, T., Fujita, N., Tsuruo, T., Miyasaka, M.
-
Journal Title
Blood. 106
Pages: 2613-2618
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
[Book] 免疫20062006
Author(s)
片桐 晃子, 木梨 達雄, 岸本忠三編
Total Pages
385
Publisher
中山書店
Description
「研究成果報告書概要(和文)」より
Related Report
