Molecular basis of odorant receptor genes expression and axonal projection of olfactory sensory neurons using zebrafish
| Project/Area Number |
17570172
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NISHIZUMI Hirofumi The University of Tokyo, Graduate School of Science, Research Associate, 大学院・理学系研究科, 助手 (30292832)
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| Co-Investigator(Kenkyū-buntansha) |
坪井 昭夫 東京大学, 大学院・理学系研究科, 助手 (20163868)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | zebrafish / odorant receptor genes / gene expression / locus control region / enhancer / 嗅覚系 / 嗅覚受容体 / 遺伝子発現 / 軸索投射 |
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| Research Abstract |
We have previously reported a 2.1kb homology (H) sequence, conserved between mouse and human, regulates the odorant receptor (OR) gene MOR28 in transgenic mice. Here, we narrowed down the essential sequences of the H to a core of 124bp, using a transient expression system in zebrafish embryos. Transgenic experiments in mice demonstrated that the core-H sequence is sufficient to endow expression of the MOR28 minigene. Deletion and mutation analyses of the core-H region revealed two homeodomain sequences to be essential for the H enhancer activity. Targeted deletion of the core-H abolished expression of three proximal OR genes, MOR28, MOR10, and MOR83, in cis, indicating the presence of another LCR/enhancer in the downstream region, that regulates four distal OR genes in the same MOR28 cluster. In the heterozygous mice, the H^- phenotype of the mutant allele was not rescued by the wild-type H^+ allele in trans.
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Report
(3 results)
Research Products
(5 results)
