| Project/Area Number |
17570176
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Kyoto University |
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Principal Investigator |
SAKAMAKI Kazuhiro Kyoto University, Graduate School of Biostudies, Associate professor, 生命科学研究科, 助教授 (20271017)
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| Co-Investigator(Kenkyū-buntansha) |
UENO Naoto National Institute for Basic Biology, Division of Morphogenesis, Professor, 基礎生物学研究所形態形成研究部門, 教授 (40221105)
YOKOTA Hideo RIKEN, Integrated V-CAD System Research Program, Team leader, 理化学研究所・VCAT開発チーム, チームリーダー (00261206)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Transgenic / Xenopus / fluorescent protein / Cre / loxP |
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| Research Abstract |
Fate maps of embryonic cells or tissues during morphogenesisare useful for understanding the development. To urge fate-mapping studies, we aimed to establish a Cre/loxP system combined with transgenesis in Xenopus frogs. Because it was expected that the Cre/loxP system with transgenic frogs shows the advantage for tracing cells or tissues over a long period. We constructed the transgene, CAG promoter-loxP/EGFP/IoxP-DsRed2 (tentatively named as EGFP/DsRed) and inserted it into fertilized eggs by microinjection. The established transgenic Xenopus line (Green/Red-Tg) ubiquitously expressed EGFP in the whole body. Furthermore, by intercrossing this line with another transgenic line expressing Cre recombinase under the control of the muscle-specific cardiac actin promoter, we found that DsRed2 fluorescent proteins were expressed in embryos in a muscle-specific manner (Waldner et al., Dev. Dyn., 2006). Thus, we showed that the Cre/loxP system works in amphibians as well as mammals, and our Green/Red Tg frogs are useful as a reporter animal. We also developed a technique by electroporationforintroducingCremRNAintoembryos.Usingourtransgenicfrogs, we are now studying how left-right (LR) positional information inducing anatomical asymmetry is determined during embryogenesis.
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