| Project/Area Number |
17570189
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Evolutionary biology
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| Research Institution | Sophia University |
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Principal Investigator |
IUCHI Ichiro Sophia University, Institute of Science and Technology, Professor (10011694)
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| Co-Investigator(Kenkyū-buntansha) |
YASUMASU Shigeki Sophia University, Institute of Science and Technology, Professor (00222357)
IUCHI Ichiro Sophia University, Institute of Science and Technology, Professor (10011694)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Gene / Genome / Evolution / Protein / Hemoglobin / Hatching Enzyme / 卵化酵素 / 蛋白質 / グロビン / メダカ / 硬骨魚 |
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| Research Abstract |
In the present research project, we clarified three characteristics of globin gene structure and expression in medaka: 1) head-to-head orientation of major α and β globin genes, 2) existence of cis-regulatory regions responsible for spatio-temporal expression of the genes 1Kb-5'upstream to the respective genes, 3) localization of the globin genes in 2 clusters on 2 chromosomes. Molecular phylogenetic analysis showed that all the teleostean globin genes were assigned into 4 groups, and in the medaka genome, the genes for one of the 4 that functions as an adult globin were pseudogenized during evolution.
In addition, we identified hatching enzyme genes in the genomes of about 30 teleosts. The results showed that the hatching enzyme genes in fishes belonging to Elopomorpha were ancestral, and were diversified into 2 groups of genes in the descendants Otocephara and Euteleostei. Especially, the EICE genes of Euteleostei would be established by "phylogeny-dependent, step-by-step intron-loss" evolution.
The evolutionary feature of globin and hatching enzyme genes reflects a characteristic of Teleost such that they could adapt in extremely diversified environments during evolution.
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