Analysis of a novel protein conjugation system relating to ubiquitin-like protein RURM1 of rice

• Project/Area Number: 17580005
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Kyoto University
• Principal Investigator: NAKAZAKI Tetsuya Kyoto University, Graduate School of Agriculture, Lecturer (60217693)
• Co-Investigator(Kenkyū-buntansha): TANISAKA Takatoshi Kyoto University, Graduate School of Agriculture, Professor (80026591) ; OKUMOTO Yutaka Kyoto University, Graduate School of Agriculture, Associate Professor (90152438) ; HORIBATA Akira Kinki University, Faculty of Biology-Oriented Science, Lecturer (70258060)
• Project Period (FY): 2005 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥3,300,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2005: ¥900,000 (Direct Cost: ¥900,000)
• Keywords: mPing / Transposon / Rurm 1 system / Rice / Mutant line IM294 / Rurmlシステム / RURM1システム

Research Abstract

Transposable activity of an active-transposon mPing in a rice mutant line, IM294 is much higher than in the original variety Gimbozu. Because IM294 is considered harboring a recessive mutation allele at Rurm1 locus, it is very likely that the destruction of the normal Rurm1 gene participates in high mPing transposable activity of IM294. We analyzed a change to occur by function loss of the Rurm1 gene to elucidate a novel rice protein conjugation system relating to the RURM1 protein (Rurm1 system), and this study was going to get the due to clarify the induction mechanism for the transposition of mPing.
At first in this study, we analyzed the influence that the function loss of Rurm1 gave transposition frequency of mPing using the transposon display method. As a result, high-frequency transposition of mPing relation to the function loss of Rum/ was confirmed in a various genetic background, and it was suggested that the function loss of Rurm1 was one of the causes to raise transposition frequency of mPing (preparing for submission). In addition, we successfully established the system to purifying RURM1 protein with bacterial recombinant protein expression system including detection of a culture condition to produce soluble non-denaturation RURM1 protein. Anti-RURM1 antibody was obtained with the purified recombinant RURM1 protein and the provided anti-RURM1 antibody confirmed specific recognition for RURM1 protein This anti-RURM1 antibody would be useful to identify proteins, which participate in a RURM1 protein conjugation system. Furthermore, in this study, a TOR (target of rapamycin) gene of the rice was identified and an expression analysis was performed. Then, it was found possibility that the protein conjugation system for RURM1 protein (urmylation) bears an essential role in the mechanism for controlling cell division in rice plant.

Research Products

• [Journal Article] Gene cloning expression, purification and characterization of rice(Oryza sativa L.)classII chitinase CHT11 (2008)
  • Author(s): Xayphakatsa, K., T. Tsukiyama, K. Inoue, Y. Okumoto, T. Nakazaki and T. Tanisaka
  • Journal Title: Enzyme Microbial Technology (in press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Active transposons in rice (2008)
  • Author(s): Nakazaki, T., K. Naito, Y. Okumoto and T. Tanisaka
  • Journal Title: Rice Biology in the Genomics Era. H.-Y. Hirano, et. al.(eds.)Springer-Verlag Berlin Heidelbelg Pages: 69-79
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Gene cloning, expression, purification and characterization of rice (Oryza sativa L.) class II chitinase CHT11 (2008)
  • Author(s): Xayphakatsa, K., T., Tsukiyama, K., Inoue, Y., Okumoto, T., Nakazaki, T., Tanisaka
  • Journal Title: Enzyme and Microbial Tchnology (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Active transposons in rice (2008)
  • Author(s): Nakazaki, T., K., Naito, Y., Okumoto, T., Tanisaka
  • Journal Title: Rice Biology in the Genomics Era. H.-Y. Hirano, et. al. (eds.) Springer-Verlag Berlin Heidelbelg Pages: 69-79
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Gene cloning expression, purification and characterization of rice (Oryza sativa L.) classII chitinase CHT11 (2008)
  • Author(s): Xayphakatsa, K., T. Tsukiyama, K. Inoue, Y. Okumoto, T. Nakazaki and T. Tanisaka
  • Journal Title: Enzyme Microbial Technology (印刷)
  • Peer Reviewed
• [Journal Article] Active transposons in rice (2008)
  • Author(s): Nakazaki, T., K. Naito, Y. Okumoto and T. Tanisaka
  • Journal Title: Rice Biology in the Genomics Era. H. -Y. Hirano, et. al. (eds.) Springer-Verlag Berlin Heidelbelg Pages: 69-79
  • Peer Reviewed
• [Journal Article] 電離放射線がmPingファミリーの転移に及ぼす効果 (2007)
  • Author(s): 岩瀬祥子・内藤健・奥本裕・築山拓司・中崎鉄也・森田美佳・谷坂隆俊
  • Journal Title: 近畿作育研究 52 Pages: 69-72
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] A novel transposon Pyong in the japonica rice variety Gimbozu (2007)
  • Author(s): Oki, N., Y. Okumoto, T. Tsukiyama, K. Naito, T. Nakazaki and T. Tanisaka
  • Journal Title: 近畿作育研究 52 Pages: 39-43
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Effects of γ-irradiation on transposition activity of the mPing family transposons (2007)
  • Author(s): Iwase, S., Y., Okumoto, T., Tsukiyama, T., Nakazaki, M., Morita, T., Tanisaka
  • Journal Title: Kinki, J. Crop Sci. Breed 52 Pages: 69-72
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A Novel transposon Pyong in the japonica rice variety Gimbozu (2007)
  • Author(s): Oki, N., Y., Okumoto, T., Tsukiyama, K., Naito, T., Nakazaki, T., Tanisaka
  • Journal Title: Kinki J. Crop Sci. Breed 52 Pages: 39-43
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Dramatic amplification of a rice transposable element during recent domestication. (2006)
  • Author(s): Naito K., E.Cho, G.Yang, M.A.Campbell, K.Yano, Y.Okumoto, T.Tanisaka, S.R.Wessler
  • Journal Title: Proc. Natl. Acad. Sci. USA 103 Pages: 17620-17625
• [Journal Article] イネ新規ユビキチン様タンパク質RURM1のクローニング,大腸菌発現および精製. (2006)
  • Author(s): 築山拓司, 李鐘源, 井上國世, 奥本裕, 中崎鉄也, 谷坂隆俊
  • Journal Title: 育種学研究 8(別1) Pages: 37-37
• [Journal Article] Different forms of mPing MITE in wild Oryza species. (2006)
  • Author(s): Karki, S., K.Naito, Y.Okumoto, T.Tsukiyama, T.Nakazaki, T.Tanisaka
  • Journal Title: Breed. Res. 8(別2) Pages: 64-64
• [Journal Article] Rurml (Rice ubiquitin-related modifier-1) の機能喪失はmPingの転移を促す. (2006)
  • Author(s): 田村佳奈子, 大木信彦, 築山拓司, 奥本裕, 中崎鉄也, 谷坂隆俊
  • Journal Title: 育種学研究 8(別2) Pages: 78-78
• [Journal Article] イネ品種銀坊主におけるmPingの急激な増殖を引き起こした原因の解明に向けて. (2006)
  • Author(s): 内藤健, 奥本裕, 門田有希, 中崎鉄也, 谷坂隆俊
  • Journal Title: 育種学研究 8(別2) Pages: 98-98
• [Journal Article] イネの活性型トランスポゾンmPingのによって誘発された量的形質関連変異のトランスポゾンディスプレイ. (2006)
  • Author(s): 堀端章, 松井和幸, 井上悦子, 吉廣卓哉, 川路英哉, 中川優, 奥本裕, 中崎鉄也, 谷坂隆俊
  • Journal Title: 育種学研究 8(別2) Pages: 127-127
• [Journal Article] イネRURM1活性化酵素遺伝子Ruba4のデータベース情報からの探索 (2005)
  • Author(s): 中崎鉄也・奥本裕・大西麻紀子・堀端章・谷坂隆俊
  • Journal Title: 近畿作育研究 50 Pages: 45-48
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Database search of Ruba4 encoding RURM1-activating enzyme (2005)
  • Author(s): Nakazaki, T., Y., Okumoto, A., Ohnishi, T., Tanisaka
  • Journal Title: Kinki J. Crop Sci. Breed 50 Pages: 45-48
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] イネRURM1活性化酵素遺伝子Ruba4のデータベース情報からの探索 (2005)
  • Author(s): 中崎鉄也, 奥本裕, 大西麻紀子, 堀端章, 谷坂隆俊
  • Journal Title: 近畿作育研究 50 Pages: 45-48
• [Journal Article] イネ非自律性トランスポゾンmPingの転移を促す遺伝要因 (2005)
  • Author(s): 大西麻紀子, 築山拓司, 奥本裕, 山平諭, 森田美佳, 中崎鉄也, 谷坂隆俊
  • Journal Title: 育種学研究 7(別1,2) Pages: 134-134
• [Journal Article] イネ品種銀坊主における新規トランスポゾンPyongの同定 (2005)
  • Author(s): 大木信彦, 奥本裕, 築山拓司, 中崎鉄也, 谷坂隆俊
  • Journal Title: 育種学研究 7(別1,2) Pages: 113-113
• [Presentation] mPingSCARマーカーはジャポニカイネ品種の分子マーカーとして極めて有効である (2007)
  • Author(s): 門田有希・内藤健・斉藤大樹・大木信彦・出田収・中崎鉄也・築山拓司, 奥本裕, 谷坂隆俊
  • Organizer: 日本育種学会第112回講演会
  • Place of Presentation: 山形大学,酒田
  • Year and Date: 2007-09-23
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] イネユビキチン様タンパク質Rurm1の機能喪失はトランスポゾンの転移活性を高める (2007)
  • Author(s): 田村佳奈子・築山拓司・奥本裕・堀端章・稲垣春香・中崎鉄也・谷坂隆俊
  • Organizer: 日本育種学会第112回講演会
  • Place of Presentation: 山形大学,酒田
  • Year and Date: 2007-09-23
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] mPing SCAR markers : a resolution to sturated linkage map construction using japonica x japonica cross (2007)
  • Author(s): Monden, Y., K., Naito, D., Saoto, N., Oki, O., Ideta, T., Nakazaki, T., Tsukiyama, Y., Okumoto, T., Tanisaka
  • Organizer: 112th Meeting of The Japanese Society of Breeding
  • Place of Presentation: Yamagata University, Sakata
  • Year and Date: 2007-09-23
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Destruction of rice ubiquitin-related modifier-1, Rurm1, enhances the transposition activity of transposable elements (2007)
  • Author(s): Tamura, K., T., Tsukiyama, Y., Okumoto, H., Horibata, H., Inagaki, T., Nakazaki, T., Tanisaka
  • Organizer: 112th Meeting of The Japanese Society of Breeding
  • Place of Presentation: Yamagata University, Sakata
  • Year and Date: 2007-09-23
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] mPing SCARマーカーはジャポニカイネ品種の分子マーカーとして極めて有効である (2007)
  • Author(s): 門田有希・内藤健・斉藤大樹・大木信彦・出田収・中崎鉄也・築山拓司, 奥本裕, 谷坂隆俊
  • Organizer: 日本育種学会第112回講演会
  • Place of Presentation: 山形大学,酒田
  • Year and Date: 2007-09-23
• [Presentation] イネユビキチン様タンパク質Rurmlの機能喪失はトランスポゾンの転移活性を高める (2007)
  • Author(s): 田村佳奈子・築山拓司・奥本裕・堀端章・稲垣春香・中崎鉄也・谷坂隆俊
  • Organizer: 日本育種学会第112回講演会
  • Place of Presentation: 山形大学,酒田
  • Year and Date: 2007-09-23
• [Presentation] Purification of ABA-induced rice class II chitinase CHT11 using bacterial recombinantprotein expression system (2007)
  • Author(s): Xayphakatsa, K., T. Tsukiyama, K. Inoue, Y. Okumoto, T. Nakazaki and T. Tanisaka
  • Organizer: 日本育種学会第111回講演会
  • Place of Presentation: 茨城大学,水戸
  • Year and Date: 2007-03-31
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Purification of ABA-induced rice class II chitinase CHT11 using bacterial recombinant protein expression system (2007)
  • Author(s): Xayphakatsa, K., T., Tsukiyama, K., Inoue, Y., Okumoto, T., Nakazaki, T., Tanisaka
  • Organizer: 111th Meeting of The Japanese Society of Breeding
  • Place of Presentation: Ibaragi University Mito
  • Year and Date: 2007-03-31
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] イネの活性型トランスポゾンmPingによって誘発された量的形質関連変異のトランスポゾンディスプレイ (2006)
  • Author(s): 堀端章・松井和幸・井上悦子・吉廣卓哉・川路英哉・中川優・奥本裕・中崎鉄也・谷坂隆俊
  • Organizer: 日本育種学会第110回講演会
  • Place of Presentation: 愛媛大学,松山
  • Year and Date: 2006-09-23
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] イネ品種銀坊主におけるmPingの急激な増殖を引き起こした原因の解明に向けて (2006)
  • Author(s): 内藤健・奥本裕・門田有希・中崎鉄也・谷坂隆俊
  • Organizer: 日本育種学会第110回講演会
  • Place of Presentation: 愛媛大学,松山
  • Year and Date: 2006-09-23
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Rurm1(Rice ubiquitin-related modifier-1)の機能喪失はmPingの転移を促す (2006)
  • Author(s): 田村佳奈子・大木信彦・築山拓司・奥本裕・中崎鉄也・谷坂隆俊
  • Organizer: 日本育種学会第110回講演会
  • Place of Presentation: 愛媛大学,松山
  • Year and Date: 2006-09-23
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Different forms of mPing MITE in wild Oryza species (2006)
  • Author(s): Karki, S., K. Naito, Y. Okumoto, T. Tsukiyama, T. Nakazaki, and T. Tanisaka
  • Organizer: 日本育種学会第110回講演会
  • Place of Presentation: 愛媛大学,松山
  • Year and Date: 2006-09-23
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 日本晴および野生イネゲノムに存在するPyong様因子の同定 (2006)
  • Author(s): 大木信彦・奥本裕・築山拓司・内藤健・中崎鉄也・谷坂隆俊
  • Organizer: 日本育種学会第110回講演会
  • Place of Presentation: 愛媛大学,松山
  • Year and Date: 2006-09-23
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Transpososon display of mutations on quantitative traits induced by the endogenous active transposon, mPing in rice (Oriza saliva L) (2006)
  • Author(s): Horibata, A., K., Matsui, E., Inoue, T., Yoshihiro, H., Kawaji, M., Nakagawa, Y., Okumoto, T., Nakazaki, T., Tanisaka
  • Organizer: 110th Meeting of The Japanese Society of Breeding
  • Place of Presentation: Ehime University, Matsuyama
  • Year and Date: 2006-09-23
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Uncovering the factor having induced the burst of mPing in a japonica rice cultivar Gimbozu (2006)
  • Author(s): Naito, K., Y., Okumoto, Y., Monden, T., Nakazaki, T., Tanisaka
  • Organizer: 110th Meeting of The Japanese Society of Breeding
  • Place of Presentation: Ehime University, Matsuyama
  • Year and Date: 2006-09-23
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Destruction of RuRm1 (Rice ubiguitin-related modifier-1) enhances the transposition activity of mPing (2006)
  • Author(s): Tamura, K., N., Oki, T., Tsukiyama, Y., Okumoto, T., Nakazaki, T., Tanisaka
  • Organizer: 110th Meeting of The Japanese Society of Breeding
  • Place of Presentation: Ehime University, Matsuyama
  • Year and Date: 2006-09-23
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Different forms of mPing MITE in wild Oryza species (2006)
  • Author(s): Karki, S., K., Naito, Y., Okumoto, T., Tsukiyama, T., Nakazaki, T., Tanisaka
  • Organizer: 110th Meeting of The Japanese Society of Breeding
  • Place of Presentation: Ehime University, Matsuyama
  • Year and Date: 2006-09-23
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Identification of Pyong-like elements in the genome of Nipponnbare and wild rice species (2006)
  • Author(s): Oki, N., Y., Okumoto, T., Tsukiyama, K., Naito, T., Nakazaki, T., Tanisaka
  • Organizer: 110th Meeting of The Japanese Society of Breeding
  • Place of Presentation: Ehime University, Matsuyama
  • Year and Date: 2006-09-23
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] イネ新規ユビキチン様タンパク質RURM1のクローニング,大腸菌発現および精製 (2006)
  • Author(s): 築山拓司・李鐘源・井上國世・奥本裕・中崎鉄也・谷坂隆俊
  • Organizer: 日本育種学会第109回講演会
  • Place of Presentation: 東京農工大学,府中
  • Year and Date: 2006-03-30
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Cloning, bacterial expression and purification of rice (Oryza saliva L.) novel ubiquitin-related protein RURM1 (2006)
  • Author(s): Tsukiyama, T., J., Lee, K., Inoue, Y., Okumoto, T., Nakazaki, T., Tanisaka
  • Organizer: 109th Meeting of The Japanese Society of Breeding
  • Place of Presentation: Tokyo University of Agriculture & Technology, Tokyo
  • Year and Date: 2006-03-30
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] GST 融合タンパク質発現系を用いた新規イネユビキチン様タンパク質 RURM1の精製 (2006)
  • Author(s): 李鐘源・谷坂隆俊・築山 拓司・奥本 裕・中崎 鉄也・井上 國世
  • Organizer: 日本農芸化学会2006年度大会
  • Place of Presentation: 京都女子大学,京都
  • Year and Date: 2006-03-26
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Purification of a novel rice ubiquitin-like protein RURM1 using GST-fusion protein expression system (2006)
  • Author(s): Lee, J., T., Tanisaka, Tsukiyama, Y., Okumoto, T., Nakazaki, T., K., Inoue
  • Organizer: Annual Meeting of JSBBA 2006
  • Place of Presentation: Kyoto Women's University, Kyoto
  • Year and Date: 2006-03-26
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] イネユビキチン様タンパク質RURM1の精製 (2005)
  • Author(s): 李鐘源・谷坂 隆俊・築山 拓司・奥本 裕・中崎 鉄也・井上 國世
  • Organizer: 日本農芸化学会2005年度 関西・中四国・西日本支部合同大会
  • Place of Presentation: 京都女子大学,京都
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Purification of a rice ubiquitin-like protein RURM1 (2005)
  • Author(s): Lee, J., T., Tanisaka, Tsukiyama, Y., Okumoto, T., Nakazaki, T.K., Inoue
  • Organizer: Annual Meeting of JSBBA Kansai, Chu-shikoku and Nishi-Nihon branch 2005
  • Place of Presentation: Osaka University, Suita
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] イネ非自律性トランスポゾンmPingの転移を促す遺伝要因 (2005)
  • Author(s): 大西麻紀子・築山拓司・奥本裕・山平諭・森田美佳・中崎鉄也・谷坂隆俊
  • Organizer: 日本育種学会第107・108回講演会
  • Place of Presentation: 筑波大学,つくば
  • Year and Date: 2005-08-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] イネ品種銀坊主における新規トランスポゾンPyongの同定 (2005)
  • Author(s): 大木信彦・奥本裕・築山拓司・中崎鉄也・谷坂隆俊
  • Organizer: 日本育種学会第107・108回講演会
  • Place of Presentation: 筑波大学,つくば
  • Year and Date: 2005-08-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Genetic factors responsible for the mobility of mPing in the rice genome (2005)
  • Author(s): Onishi, A., T., Tsukiyama, Y., Okumoto, S., Yamahira, M., Morita, T., Nakazaki, T., Tanisaka
  • Organizer: 107, 108th Meeting of The Japanese Society of Breeding
  • Place of Presentation: Tsukuba University, Tsukuba
  • Year and Date: 2005-08-21
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Identification of a novel transposon Pyong in japonica rice variety Gimbozu (2005)
  • Author(s): Oki, N., Y., Okumoto, T., Tsukiyama, T., Nakazaki, T., Tanisaka
  • Organizer: 107, 108th Meeting of The Japanese Society of Breeding
  • Place of Presentation: Tsukuba University, Tsukuba
  • Year and Date: 2005-08-21
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Distribution of Pong, Ping and mPing transposons in NERICA rice (2005)
  • Author(s): Karki, S., Y. Okumoto, T. Nakazaki and T. Tanisaka
  • Organizer: Fifth International Rice Genetics Symposium and 3rd International Rice Functional Genomics Symposium
  • Place of Presentation: Edas Shangri-La Hotel, Manila
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Investigation of a mPing-transposition mechanism using a mutant line with hightransposition activity (2005)
  • Author(s): Nakazaki, T.
  • Organizer: The 6th Kyoto University International Symposium"Plant Sciences in Japan and China-from genetics to Breeding"
  • Place of Presentation: Chinese Academy of Agricultural Sciences, Beijing
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Distribution of Pong, Ping and mPing transposons in NERICA rice (2005)
  • Author(s): Karki, S., Y., Okumoto, T., Nakazaki, T., Tanisaka
  • Organizer: Fifth International Rice Genetics Symposium and 3rd International Rice Functional Genomics Symposium
  • Place of Presentation: das Shangri-La Hotel, Manila
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Investigation of a mPing-transposition mechanism using a mutant line with high transposition activity (2005)
  • Author(s): Nakazaki, T
  • Organizer: The 6th Kyoto University International Symposium "Plant Sciences in Japan and China - from genetics to Breeding
  • Place of Presentation: Chinese Academy of Agricultural, Sciences, Beijing
  • Description: 「研究成果報告書概要(欧文)」より