| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Research Abstract |
Phylogenetic analysis of 18 species of Xanthomonas was performed by using 23S rRNA, ITS region and gyrB gene. Based on partial sequences of 23S rDNA, Xanthomonas species were divided into two groups. Members of each group were similar to those of 16S rDNA analysis. Group 1 was a major group which was composed of 14 species, while group 2 was consisted of 4 species, X.albilineans, X.hyacinthi, X.sacchari and X.translucens. The similarities between two groups were ranged from 96.6 to 97.8 %, but those within same group were more than 98.8 %. According to ITS sequences, ITS was divided into three regions ITS 1, 2, and 3 by existence of two tRNAs, tRNA^<Ala> and tRNA^<Ile>. The length of each ITS region was 84-96 nt, 14-76 nt, and 222-228 nt, respectively. The length of ITS was very diverse among Xanthomonas species. Although two tRNAs were highly conserved, they were classified into two types by slight differences in sequences. In tRNA^<Ala>, two nucleotides were different between two types, and three nucleotides were different in tRNA^<Ile>. On the basis of ITS sequences, Xanthomonas species were also divided into two groups. Members of each group were the same as those of 23S groups.
Based on partial sequences of gyrB, Xanthomonas species were divided into two groups, which were the same as those based on 23S and ITS sequences. A phylogenetic tree showed that analysis of gyrB was a match for genomic fingerprints. In Xanthomonas, phylogenetic analysis based on gyrB may be the one best suited to the identification of bacterial strains at the genomic species level.
In conclusion, the results of this study demonstrated that phylogenetically, the Xanthomonas strains tested may be grouped into two lineages.
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