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Solubilization of organic phosphate by arbuscular mycorrhizal fungi

Research Project

Project/Area Number 17580051
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionYamagata University

Principal Investigator

TAWARAYA Keitaro  Yamagata University, Faculty of Agriculture, Professor, 農学部, 教授 (70179919)

Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsarbuscular mycorrhizal fungi / phosphate / phosphatase / hyphae / soil organic matter / hyphal exudate / hyphosphere / Glomus / 菌根 / 酸性ホスファターゼ / 外生菌糸 / 有機態リン酸 / 不可給態リン酸 / リン / 共生 / Clomus
Research Abstract

Sterilized Andisol was packed in pots that were separated into the mycorrhizal and hyphal compartments with a mesh bag of 30 μm pore size. Onion (Allium cepa L.) inoculated with AM fungus, Glomus clarum were grown in a growth cabinet. The control plants were not inoculated. The mulite ceramic tubes connected with a Teflon tube were buried in the soil of both compartments before sowing. Soil solution was collected from each compartment through the tubes. Acid phosphatase activity in the solutions was assayed based on the amount of p-nitrophenol released by hydrolysis of p-nitrophenylphosphate. For the qualitative analysis of phosphatase, the soil solutions were concentrated and loaded on a SDS-polyacrylamide gel, and acid phosphatase activity was detected by the azo dye method.
Onion plants were well colonized with AM fungi. Shoot phosphorus concentration was higher in the onion plants inoculated with G. clarum than that in the uninoculated plants. Acid phosphatase activities in the soil solutions collected from the mycorrhizal and hyphal compartments of the mycorrhizal plants were higher than those of the uninoculated plants. Mycorrhiza-specific phosphatase activity was detected by the electrophoretic analysis. These observations suggest that not only the exploitation of bulk soil by the external hyphal network of mycorrhizal fungi but also the exudation of acid phosphatase contribute to the enhanced uptake of phosphate in mycorrhizal plants.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (9 results)

All 2006

All Journal Article (9 results)

  • [Journal Article] Solubilization of insoluble inorganic phosphate by hyphal exudates of arbuscular mycorrhizal fungi.2006

    • Author(s)
      Tawaraya et al.
    • Journal Title

      Journal of Plant Nutrition 29

      Pages: 657-665

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Arbuscular mycorrhizal fungi increased early growth of two nontimber forest product species Dyera polyphylla and Aquilaria filaria under greenhouse conditions2006

    • Author(s)
      Turjaman et al.
    • Journal Title

      Mycorrhiza 16

      Pages: 459-464

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Increase in early growth and nutrient uptake of Shorea seminis seedlings inoculated with two ectomycorrhizal fungi2006

    • Author(s)
      Turjaman et al.
    • Journal Title

      Journal of Tropical Forest Science 18

      Pages: 243-249

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Solubilization of insoluble inorganic phosphate by hyphal exudates of arbuscular mycorrhizal fungi2006

    • Author(s)
      Tawaraya, K., Naito, M., Wagatsuma, T.
    • Journal Title

      Journal of Plant Nutrition 29

      Pages: 657-665

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Arbuscular mycorrhizal fungi increased early growth of two nontimber forest product species Dyera polyphylla and Aquilaria filaria under greenhouse conditions2006

    • Author(s)
      Turjaman, M., Tamai, Y., Santoso, E., Osaki, M., Tawaraya, K.
    • Journal Title

      Mycorrhiza 16

      Pages: 459-464

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Increase in early growth and nutrient uptake of Shorea seminis seedlings inoculated with two ectomycorrhizal fungi2006

    • Author(s)
      Turjaman, M., Tamai, Y., Segah, H., Limin, S.H., Osaki, M., Tawaraya, K.
    • Journal Title

      Journal of Tropical Forest Science 18

      Pages: 243-249

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Solubilization of insoluble inorganic phosphate by hyphal exudates of arbuscular mycorrhizal fungi2006

    • Author(s)
      Tawaraya, K, Naito, M, Wagatsuma, T
    • Journal Title

      Journal of Plant Nutrition 29

      Pages: 657-665

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Arbuscular mycorrhizal fungi increased early growth of two nontimber forest product species Dyera polyphylla and Aquilaria filaria under greenhouse conditions2006

    • Author(s)
      Turjaman, M, Tamai, Y, Santoso, E, Osaki, M, Tawaraya, K
    • Journal Title

      Mycorrhiza 16

      Pages: 459-464

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Solubilization of insoluble inorganic phosphate by hyphal exudates of arbuscular mycorrhizal fungi.2006

    • Author(s)
      Tawaraya et al.
    • Journal Title

      Journal of Plant Nutrition (in press)

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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