| Project/Area Number |
17580060
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied microbiology
|
|---|
| Research Institution | Tokyo Institute of Technology |
|---|
Principal Investigator |
KAJIWARA Susumu Tokyo Institute of Technology, Graduate school of Biosci. & Biotech., Assoc. Professor, 大学院生命理工学研究科, 助教授 (10272668)
|
|---|
| Project Period (FY) |
2005 – 2006
|
| Project Status |
Completed (Fiscal Year 2006)
|
| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2005: ¥2,300,000 (Direct Cost: ¥2,300,000)
|
|---|
| Keywords | fatty acid / desaturase / gene expression / stress response / yeast / analysis of promoter |
|---|
| Research Abstract |
To clarify what kind of stress environments the fatty acid desaturases genes (Sk-OLE1,Sk-FAD2,Sk-FAD3) of yeast Saccharomyces kluyveri is induced by and where the cis-elements for gene induction on the promoter area exist in, the transcription of these desaturase genes was researched. The results are shown below.
1)It was shown that these three fatty acid desaturase genes do not response by the addition of unsaturated fatty acid
2)All the transcription of these three genes were induced by low oxygen environment and their induction patterns were different each other.
3)Ii was suggested that Sk-FAD2 and SK-FAD3 are regulated by only low temperature stress. On the other hand, Sk-OLE1 are regulated by not only low temperature stress but also cell membrane situation
4)It was suggested that the necessary area for the low-temperature induction exists between the upstream 228-172bp of the Sk-OLEI structural gene. Moreover, in this area, there is a CCCCT sequence that was called STRE (stress response element) and then the possibility that STRE took part in the low-temperature induction of Sk-OLE1 gene was thought.
5)It was suggested that the necessary area for the low-temperature induction exists between upstream 326-209bp of the Sk-FAD2 structural gene. In the upstream 228-172bp of this gene, it was shown that there is the T-rich region.
6)It was suggested that the necessary area for the low-temperature induction exists between upstream 207-145bp of the Sk-FAD3 structural gene. In this area, there is no common region with the low-temperature response areas in the upstream of the Sk-OLE1 gene and the Sk-FAD2 gene although there is a CCAAT-box in this area.
The portion of the stress response mechanism of yeast S kluyveri became clear by the above-mentioned result of this research.
|
