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Are rare earth elements essential for growth of microorganisms?

Research Project

Project/Area Number 17580063
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionGifu University

Principal Investigator

KAWAI Keiichi  Gifu University, Faculty of applied biological thences, Professor (00002064)

Co-Investigator(Kenkyū-buntansha) IWAMA Tomonori  Gifu university, Faculty of applied biological sciences, Associate professor (60252128)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,040,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsRare earth element / Methvlobacterium / Nevskin rnmosa / Sphinomonas / rare earth responsive microorganisms / Europium / Lanthanum / Cerium / 軽希土類元素 / La / Ce / Sm / メタノールの増殖促進 / メタノール脱水素酵素 / 微生物 / サマリウム / Methlobacterium / 増殖促進 / Eu
Research Abstract

The rare earth elements have been used for various high technology products. We have been screening microorganisms which show a peculiar growth in the presence of rare earth elements. We had isolated successfully some rare-earth responsive microorganisras(sliains EU-1, EU-2 and EU-3). In thisresearch, we investigated on three strains (EU-1, EU-2 and EU-3) which formed the bigger colony size in the presence of europium (Eu). Strain EU-1 was identified as Methylobactetizun sp. The growthof this strain was stimulated by Sm on 1/100 nutrient agar plate with methanol. The growth of this bacterium increased about 10-times when Sm was added to the liquid medium, suggesting that Sm was involved in the methanol metabolism of this bacterium. Strain EU-2 was identified as Nevskia ramosea. The growth of strain EU-2 was found to be increased by Eu on a soft agar: This indicated that the mobility of this bacterium was controlled by Eu. Although the viable cells were increased 20 times by adding Eu t … More o the medium, some dispersion of the colony munber appeared on the plate was found. This was due to the aggregation of the cells especially in the absence of Eu Strain EU-3, identified as Sphingomonas sp. EU-3, when the cell growth was reached to stationary phase, immediately the cells went into the death phase. La addition to the medium, however, the stationary phase in was continued. La have uhvsiolotlically important role in survival of strain EU-3.
Methylobactelium sp.EU-1 was cultivated in the medium containing methanol and light rare earth elements, activity of methanol dehydrogenase in crude extracts of Afethylkobacterium sp. EU-1 was markedly increased. Especially La or Ce caused the increment of methanol dehydrogenase activity about 150 times. The dehydrogenase was purified.The purified enzyme migrated as a single band on SDS-PAGE. The N-terminal amino acid sequence of the dehydrogen-ase showed the high similarity with methanol dehydrogenase or PQQ-dependent dehydrogenase of Methylobacteiitun spp. Furthermore, the absorption spectra of the dehydrogenase revealed that this dehydrogenase was a member of PQQ-dependent dehydrogenase family.
These results indicated a biological importance both in viva and in vitro of rare earth elements and will give a great impact to the fields of bioinorganic chemistry. Less

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (22 results)

All 2008 2007 2006 2005

All Journal Article (18 results) (of which Peer Reviewed: 2 results) Presentation (3 results) Book (1 results)

  • [Journal Article] Application of Rare Earths in Agriculture(in Japanese)2008

    • Author(s)
      K. Kawai
    • Journal Title

      Handbook of Rare Earth Materials and Technology NTS Co. Ltd

      Pages: 978-978

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The rare earth, scandium, causes antibiotic overproduction in Streptomyces spp.2007

    • Author(s)
      K. Kawai, G. Wang, S Okamoto & K. Ochi
    • Journal Title

      FEMS Microbiol. Lett. 274

      Pages: 311-315

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The rare earth, scandium, causes antibiotic overproduction in Streptoznyces spp2007

    • Author(s)
      K. Kawai
    • Journal Title

      FEMS Microbiol Lett.(査読 有) 274

      Pages: 311-315

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The rare eairth, scandium, causes antibiotic overproduction by Streptomyces spp.2007

    • Author(s)
      Kawai K., Wang G., Okamoto S. & Ochi K.,
    • Journal Title

      FEMS Microbiol. Lett. 274

      Pages: 311-315

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bio Separation of Rare Earth Elements using Microorganisms2006

    • Author(s)
      K. Kawai, T. Iwama & T. Suzuki
    • Journal Title

      PROCEEDING The 2nd Biannual Meeting on Bioprocess Engineering

      Pages: 15-24

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Bio Separation of Rare Earth Elements using Microorganisms2006

    • Author(s)
      K. Kawai
    • Journal Title

      Proceeding The 2nd Biannual Meeting on Bioprocess Engineering(査読 無)

      Pages: 15-24

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Sm存在下におけるMethylobacterium sp. EU-1の培養特性2006

    • Author(s)
      河合啓一, 佐久間隆介, 岩間智徳, 鈴木徹
    • Journal Title

      日本農芸化学会2006年度大会要旨集

      Pages: 253-253

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Sm存在下におけるMethylobacterium sp. EU-1の増殖挙動2006

    • Author(s)
      奥田雅代, 河合啓一, 佐久間隆介, 岩間智徳, 鈴木徹
    • Journal Title

      希土類 48

      Pages: 90-91

    • NAID

      10019257072

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Eu存在下におけるNevskia ramos EU-2の増殖挙動2006

    • Author(s)
      平野朱音, 河合啓一, 岩間智徳, 鈴木徹
    • Journal Title

      希土類 48

      Pages: 92-93

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Bio Seperation of Rare Earth Elements Using Microorganisms2006

    • Author(s)
      Keiichi Kawai, Tomonori Iwama, Tohru Suzuki
    • Journal Title

      Proceeding The 2^nd Biannual Meeting on Bioprocess Engineering

      Pages: 15-24

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effect of Sc on pigment production of Fusarium solani SC-22006

    • Author(s)
      Shinya Yamaguchi, Naoko Ichigo, Keiichi Kawai, et al.
    • Journal Title

      Symposium on Panascopic Assembling and Highly Ordered Functions for Rare Earth Materials

      Pages: 30-30

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Proteome Analysis of Bradyrhizobium sp. CE-3 in the Presence and Absenc of Ce or Mannitol2006

    • Author(s)
      Mako Fushimi, Michiko Hiraoka, Keiichi Kawai, et al.
    • Journal Title

      Symposium on Panascopic Asemling and Highly Ordered Functions for Rare Earth Materials

      Pages: 61-61

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effect of Rare Earth Elements on Growth Behaviors of Microorganisms2005

    • Author(s)
      K. Kawai, T. Suzuki & T. Iwama
    • Journal Title

      Microorganisms and Human Well-Being

      Pages: 174-177

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effect of Rare Earth Elements on Growth Behaviors of Microorganisms2005

    • Author(s)
      K. Kawai, T. Suzuki, T. Iwama
    • Journal Title

      Microorganisms and Human Well-Being (査読 無)

      Pages: 174-177

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Methylobacterium sp.EU-1の増殖に及ぼす希土類元素の影響2005

    • Author(s)
      河合啓一, 佐久間隆介, 大前亜矢子, 岩間智徳, 鈴木徹
    • Journal Title

      希土類 46

      Pages: 78-79

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effect of rare earths on growth behavior of microorganisms2005

    • Author(s)
      Kawai K., Suzuki T., Iwama T.
    • Journal Title

      Microorganisms and Human Well-Being

      Pages: 174-177

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 微生物の二次代謝に及ぼすScの影響2005

    • Author(s)
      河合啓一, 岩間智徳, 山口真也, 鈴木徹, 市古奈緒子, 越智幸三
    • Journal Title

      日本生物工学会大会講演要旨

      Pages: 119-119

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Eu存在下におけるNevskia ramosa EU-2の増殖挙動2005

    • Author(s)
      平野朱音, 川澄剛士, 高野知子, 岩間智徳, 鈴木徹, 河合啓一
    • Journal Title

      日本農芸化学会大会講演要旨

      Pages: 219-219

    • NAID

      10019257074

    • Related Report
      2005 Annual Research Report
  • [Presentation] Growth Behavior of Methylobacterium sp. EU-1 in the presence of Sm2007

    • Author(s)
      K. Kawai, M. Okuda, T. Iwama & T. Suzuki
    • Organizer
      The Fifth International Conference on Rare Earth Development and Application
    • Place of Presentation
      Baotou, China
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Growth behavior of Methylobactalium sp. EU-1 in the presence of Sm2007

    • Author(s)
      K. Kawai, M. Okuda, T. Iwama, T. Suzuld
    • Organizer
      The Fifth International Conference on Rare Earth Development and Application
    • Place of Presentation
      Baotou, China
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Growth behavior of Methylobacterium sp. EU-1 in the presence of Sm2007

    • Author(s)
      Kawai K., Okada M. Iwama T., & Suzuki T.
    • Organizer
      The Fifth International Conference of Rare Earth Development and Application
    • Place of Presentation
      Bautou(China)
    • Related Report
      2007 Annual Research Report
  • [Book] 希土類の材料技術ハンドブック(分担執筆)2008

    • Author(s)
      河合 啓一(分担執筆)
    • Total Pages
      978
    • Publisher
      (株)エヌ・ティ・エス
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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