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Improvement of acidic soils by aluminium (Al)-resistant fungi and molecular mechanism of Al-resistance

Research Project

Project/Area Number 17580065
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionOkayama University

Principal Investigator

KAWAI Fusako  Okayama University, Research Institute for Bioresources, Professor (60118007)

Co-Investigator(Kenkyū-buntansha) KIMBARA Kazuhide  OKAYAMA UNIVERSITY, Research Institute for Bioresources, Associate Professor (30225122)
TANI Akio  OKAYAMA UNIVERSITY, Research Institute for Bioresources, Assistant Professor (00335621)
清水 頼子  岡山大学, 資源生物科学研究所, 技術職員 (70379808)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,610,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsacidic soil / Acd- and aluminium-resistant microbes / Penicillium janthenellum F-13 / Rhodotorula glutinis IFO 1125 / mitochondria / novel aluminium-resistant mechanism / aluminium toxicity / Al耐性菌 / Al耐性分子機構 / Al耐性カビ / Al耐性酵母 / Al耐性遺伝子 / アムミ耐性菌 / Penicillium janthinellum / Rhodotorula glutinis / アルミ耐性遺伝子 / 後成的アルミ耐性機構 / アルカリ化菌 / 活性酸素 / アルミ耐性菌
Research Abstract

1) We showed that Penicillium janthinellum F-13isolated from tea field could neutralize acidity of acidic soils in Tohoku Area of Japan and promoted germination and growth of vegitation. Efficiency was maintained for a few years. Another experimental execution of works are on going in another site in Japan, showing the similar improvement. In general, the strain is expected to improve vegitation in acidic soils of Japan.
2) We introduced two candidate Al-tolerant genes of Al-tolerant UV-mutant derived from P. chrysogenum IFO4626 into Nicotiana tabacum, designating this as TO. Using T2seeds, increase of Al-tolerance was examined by germination and growth, but no positive results have not been detected. We will try another candidate genes and another growth conditions.
3) DDGE analyses of soils collected from different sites in Thailand suggested that less microbial flora were detectable in acidic soils than in normal soil. When soil extracts were kept under acidic conditions, variety of m … More icrobial flora of regular soil was simplified, but no change was found with acidic soils, suggesting that only tolerant flora to Al and acidity exist in acidic soil and fungi are more tolerant to Al and acidity than bacteria.
4) DGGE analyses of domestic soils collected from different sites in tea fields of Shizuoka Prefecture showed that microbial flora were greatly influenced by soil pHs. Nitrogen fertilizer reduced soil pH and bacteria/fungi ratio increased under pH4. Characteristic fungi were detected in furrow soils where nitrogen fertilizers are given..
5) The mechanism of novel Al tolerance found in Rhodotorula glutinis IFO1125 was analyzed. Increased tolerance was obtained accordingly to increased Al concentrations. Increased resistat cells had increased mitochondria and their DNA. Al ion increased membrane potential of mitochondria producing active oxygen species caused by lowered cytochrome oxidase and resulting in Al toxicity. The increment of mitochondrial numbers and their DNA is concluded to be a conpensation for lowered respiration ability caused by decrement of cytochrome oxidase.
6) Involvent of three genes were suggested in Al tolerance of R. glutinis. Laccase is possibly related to iron uptake, which is elevated in the presence of Al. Arsenite-translocating ATPase is relevant to maintaining membrane integrity, especially mitochondrial function. Ca^<2+>/calmodulin-dependent protein kinase is suggested to be involved in Al tolerance through Ca^<2+>/calmodulin-dependent signaling pathway. Less

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (20 results)

All 2008 2007 2006 2005

All Journal Article (7 results) (of which Peer Reviewed: 2 results) Presentation (11 results) Book (2 results)

  • [Journal Article] The crucial role of mitochondrial regulation in adaptive aluminium resistance in Rhodtorula glutinis2008

    • Author(s)
      A. Tani, et al.
    • Journal Title

      Microbiology 154

      Pages: 3437-3446

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The crutial role of mitochondrial regulation in adaptive aluminium resistance in Rhodotorula glutinis2008

    • Author(s)
      A. Tani, C. Inoue, Y. Tanaka, Y. Yamamoto, H. Kondo, S. Hiradate, K. Kimbara, F. Kawai
    • Journal Title

      Microbiology 154

      Pages: 3437-3446

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Analysis of microbial floras in Japanese tea field's soils enriched with nitrogen fertilizers2007

    • Author(s)
      Y. Shimizu, et al.
    • Journal Title

      Proceedings of The 3rd International Conference on〓 Pr-P-305

      Pages: 1-6

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Analysis of microbial floras in Japanese tea field's soils enriched with nitrogen fertilizers2007

    • Author(s)
      Y. Shimizu, A. Morita, M. Narushima, Y. Nakamura, K. Kimbara, F. Kawai
    • Journal Title

      Proceedings of the 3^<rd> International Conference on O-cha (Tea) Cuture and Science (ICOS 2007) (ICOS 2007), Pr-P-305

      Pages: 1-6

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 101. 極酸性泥岩のアルミ耐性菌による土壌改良と緑化2006

    • Author(s)
      横山, 土居, 河合
    • Journal Title

      日緑工誌 32

      Pages: 223-226

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Improvement of strong acidic soil and vegitation by aluminum-tolerant microbes2006

    • Author(s)
      Y. Yokoyama, Y. Doi, F. Kawai
    • Journal Title

      J. Jpn. Soc. Reveget. Tech. 32(1)

      Pages: 223-226

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 極酸性泥岩のアルミ耐性菌による土壌改良と緑化2006

    • Author(s)
      横山, 土居, 河合
    • Journal Title

      日緑工誌 32・1

      Pages: 223-226

    • Related Report
      2006 Annual Research Report
  • [Presentation] Analysis of microbial floras in Japanese tea field's soils enriched with nit2007

    • Author(s)
      Y. Shimizu, A. Morita, M. Marushima
    • Organizer
      The 3rd Internaional Conference on O-cha (Tea) Culture and Sci
    • Place of Presentation
      Shizuoka
    • Year and Date
      2007-11-03
    • Related Report
      2007 Annual Research Report
  • [Presentation] Analysis of microbial floras in Japanese tea field's soils enriched with nitrogen fertiliz〓2007

    • Author(s)
      Y. Shimizu, A. Morita, M. Narush
    • Organizer
      3rd International Conference on 0-cha (Tea) Culture and Scie〓
    • Place of Presentation
      Shizuoka
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 赤色酵母Rhodotorula glutinis IF0 1125株ののアルミニウム耐性におけるミトコンドリアの役割2007

    • Author(s)
      井上, 谷, 金原, 河合
    • Organizer
      日本農芸化学会中四国支部例会
    • Place of Presentation
      香川
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Rhodotorula glutinisにおけるアルミニウム耐性機構とミトコンドリア活性2007

    • Author(s)
      谷, 井上, 金原, 河合
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] アンダーグラウンドの微生物たち2006

    • Author(s)
      河合, 谷, 清水
    • Organizer
      日本農芸化学会大会シンポジウム
    • Place of Presentation
      京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 低pH、高濃度アルミニウム土壌で生きる微生物たち2006

    • Author(s)
      河合, 谷, 清水
    • Organizer
      日本農芸化学会大会シンポジウム
    • Place of Presentation
      京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Rhodotorula glutinisにおけるアルミニウム耐性とミトコンドリア形態の関連について2006

    • Author(s)
      谷, 井上, 川原, 金原, 河合
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 赤色酵母Rhodotorula glutinisのアルミニウム耐性に関わる遺伝子のクローニング2006

    • Author(s)
      川原, 谷, 井上, 金原, 河合
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 弱酸性およびアルミニウム負荷に対するタイ北部土壌の微生物群集の挙動変化2006

    • Author(s)
      清水, P. Chantanawakul, S. Lumyo
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 極酸性泥岩のアルミ耐性菌による土壌改良と緑化2006

    • Author(s)
      横山, 土居, 河合
    • Organizer
      日本緑化工学会大会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Adaptive Al-resistance in Rhodotorula glutinis IF011252005

    • Author(s)
      A. Tani, T. Kawahara, Y. Yamamoto
    • Organizer
      The XXIInd International Conference on Yeast Genetics and Mo〓
    • Place of Presentation
      Bratislava, Slovakia
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 特殊環境微生物の発見-酸性土壌を緑地・耕地にするバイオレメディエーション2008

    • Author(s)
      惣田いく夫, 河合富佐子編
    • Total Pages
      150
    • Publisher
      合同出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Findings of Microbes Capable of Growing in Extreme Environment-Bioremediation to change Acidic Soils to Meadows and Fields2008

    • Author(s)
      Sota, F. Kawai (eds)
    • Publisher
      Godo Publishing Co.
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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