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Studies on the Mechanism of Purinenucleotide Biosynthesis and Permeability

Research Project

Project/Area Number 17580070
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionKyoto Prefectural University

Principal Investigator

MATSUI Hiroshi  Kyoto Prefectural University, Graduate School of Agriculture, Professor, 農学研究科, 教授 (40336730)

Co-Investigator(Kenkyū-buntansha) WATANABE Kunihiko  Kyoto Prefectural University, Graduate School of Agriculture, Associate Professor, 農学研究科, 助教授 (90184001)
TSUJIMOTO Yoshiyuki  Kyoto Prefectural University, Graduate School of Agriculture, Assistant Professor, 農学研究科, 助手 (20315930)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsPurinenucleotide Biosynthesis / Purinenucleotide Production / Purinenucleoside Production / Bacillus subtilis / Corynebacterium ammoniagenes / E.coli / Glucose-6-P isomerase / Glucose-6-P dehydrogenase / Corynebacterium ammoniagenes / 好熱性細菌
Research Abstract

Our final research purpose is to investigate ability to produce purine nucleotide or nucleoside in three kinds of bacteria, Bacillus subtilis, Corynebacterium ammoniagenes, and Escherichia coli. Thus, we have carried out biochemical analysis of metabolic flux in glycolysis and pentose phosphate cycles, glucose uptake system, and excretion mechanism of purine nucleotide. The results obtained until now are described below.
1. We obtained the enzymatic parameter data of glucose-6-phosphate isomerase and glucose-6-phosphate dehydrogenase related to the distribution of glucose-6-phosphate, in order to investigate the properties of metabolic flux in glycolysis and pentose phosphate cycles into purine nucleotide biosynthesis. Furthermore, we analyzed an intracellular glucose-6-phosphate isomerase and glucose-6-phosphate dehydrogenase, and an intracellular pool of glucose-6-phosphate. From these results, we can see capability of the metabolic flux to purine nucleotide biosynthesis in order of Bacillus subtilis> Corynebactrium glutamicum> E.coli.
2. We investigated inosine-producing ability in E.coli, using the breeding method by gene engineering. It was shown that inosine productivity by improving only purine nucleotide biosynthetic pathway was very low in E.coli. The inosine productivity could be improved by shutdown of glycolysis and Entner-Doudoroff pathways. Furthermore, the existence of over three kinds of inosine-degrading enzymes was found, and it was suggested that these enzymes were an important factor on inosine productivity.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (9 results)

All 2007 2006 2005

All Journal Article (9 results)

  • [Journal Article] Effect of Amplification of Desensitized purF and prs Genes on Inosine Accumulation in Escherichia coli2007

    • Author(s)
      M.Shimaoka, H.Matsui, et al.
    • Journal Title

      J. Biosci. Bioeng. 103(3)

      Pages: 255-261

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effect of Amplification of Desensitized purF and prs Genes on Inosine Accumulation in Escherichia coli2007

    • Author(s)
      M.Shimaoka, H.Matsui et al.
    • Journal Title

      J. Biosci. Bioeng. 103(3)

      Pages: 255-261

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effect of Amplification of Desensitized purF and prs Genes on Inosine Accumulation in Escherichia coli2007

    • Author(s)
      Megumi Shimaoka, Hiroshi Matsui, et al.
    • Journal Title

      J. Biosci. Bioeng. 103(3)

      Pages: 255-261

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effects of xapA and guaA Disruption on Inosine Accumulation in Escherichia coli2006

    • Author(s)
      M.Shimaoka, H.Matsui, et al.
    • Journal Title

      Biosci. Biotechnol. Biochem. 70(12)

      Pages: 3069-3072

    • NAID

      10018524313

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of xapA and guaA Disruption on Inosine Accumulation in Escherichia coli2006

    • Author(s)
      M.Shimaoka, H.Matsui et al.
    • Journal Title

      Biosci. Biotechnol Biochem. 70(12)

      Pages: 3069-3072

    • NAID

      10018524313

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of xapA and guaA Distruption on Inosine Accumulation in Escherichia coli2006

    • Author(s)
      Megumi Shimaoka, Hiroshi Matsui, et al.
    • Journal Title

      Biosci. Biotechnol. Biochem. 70(12)

      Pages: 3069-3072

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effects of edd and pgi Disruptions on Inosine Accumulation in Escherichia coli2005

    • Author(s)
      M.Shimaoka, H.Matsui, et al.
    • Journal Title

      Biosci. Biotechnol. Biochem. 69(7)

      Pages: 1248-1255

    • NAID

      130000030263

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of edd and pgr Disruptions on Inosine Accumulation in Escherichia coli2005

    • Author(s)
      M.Shimaoka, H.Matsui et al.
    • Journal Title

      Biosci. Biotechnol. Biochem. 69(7)

      Pages: 1248-1255

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of edd and pgi Disruptions on Inosine Accumulation in Escherichia coli2005

    • Author(s)
      Megumi Shimaoka, Hiroshi Matsui et al.
    • Journal Title

      Biosci.Biotechnol.Biochem. 69(7)

      Pages: 1248-1255

    • NAID

      130000030263

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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