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Distribution analysis and the application of the nitrifying bacteria which inhabits in

Research Project

Project/Area Number 17580073
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionNihon University

Principal Investigator

TAKAHASHI Reiji  Nihon University, College of Bioresource Sciences, Associate Professor, 生物資源科学部, 助教授 (70197193)

Co-Investigator(Kenkyū-buntansha) TOKUYAMA Tatsuaki  Nihon University, College of Bioresource Sciences, Professor, 生物資源科学部, 教授 (90059684)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsnitrifying bacteria / rhizoplane / phylogenetic analysis / distribution / community structure / norB / cbbL / pyk / gap / pgk
Research Abstract

In this research we focused on plant rhizoplane of plants which utilize different formed nitrogen compounds ("ammonia plant": rice, blueberry and so on, "nitrate plant"; barley, eggplant and so on), to designate the distribution of nitrifying bacteria and to definite dominant strain in these environment as final accomplishment. Recently for non-cultured analysis of ammonia-oxidizing bacteria (AOB) from several environmental sources such as DGGE, the CTO primer and the specific region originated from ammonia mono oxygenase (AMO) gene which is specific to AOB as another available primer. We tried to develop the other primers of more effective and high resolution for nitrifying bacteria analysis and to establish more efficient analysis.
<Research result>
(1) Development of unique probe for nitrifying bacteria screening : We examined that norB (nitrite oxidoreductase gene) showed the possibility of minute classification internal genus Nitrobacter of nitrite ozidizing bacteria (NOB). We showed that three of housekeeping gene ( gap, pgk, pyk), especially pyk phylogenetic tree of the AOB showed high resolution compared with the tree based on 16SrRNA gene sequence. It made that 3 subclasses of Nitrosospira, Nitrosolobus and Nitrosovibrio based on their morphological difference which it cannot identify with classification of 16SrRNA gene sequence.
(2) Analysis of distribution on the plant rhizoplane : On the basis of the previous section, for DGGE analysis, we attempt to generate the primers.
(3) Pure isolation, its breeding and functional analysis of new isolate : Newly isolated AOB and NOB were obtained from the rhizoplane of rice, blueberry, barley and eggplant. Especially the strain isolated from rhizoplae of leucaena grown in fields of an acid sulfate soil in Thailand which showed high urease activity and copper tolerance, so is very useful strain for functional analysis.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (7 results)

All 2007 2006 2005

All Journal Article (6 results) Book (1 results)

  • [Journal Article] Characteristics of newly isolated ammonia-oxidizing bacteria from acid sulfate soil and the rhizoplane of leucaena grown in that soil2007

    • Author(s)
      Kazuo Satoh, Chieko Itoh, Dong-Jin Kang, Hiroaki Sumida, Reiji Takahashi, Katsunori Isobe, Satohiko Sasaki, Tatsuaki Tokuyama
    • Journal Title

      Soil Science and Plant Nutrition 53

      Pages: 23-31

    • NAID

      110006532361

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Characteristics of newly isolated ammonia-oxidizing bacteria from acid sulfate soil and the rhizoplane of leucaena grown in that soil2007

    • Author(s)
      Kazuo Satoh, Chieko Itoh, Dong-jin Kang, Hiroaki Sumida, Reiji Takahashi, Katsunori Isobe, Satohiko Sasaki, Tatsuaki Tokuyama
    • Journal Title

      Soil Science and Plant Nutrition 53

      Pages: 23-31

    • NAID

      110006532361

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Characteristics of newly isolated ammonia-oxidizing bacteria from acid sulfate soil and the rhizoplane of leucaena grown in that soil,2007

    • Author(s)
      Kazuo Satoh, Chieko Itoh, Dong-Jin Kang, Hiroaki Sumida, Reiji Takahashi, Katsunori Isobe, Satohiko Sasaki, Tatsuaki Tokuyama
    • Journal Title

      Soil Science and Plant Nutrition 53

      Pages: 23-31

    • NAID

      110006532361

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Classification of chemoautotrophic ammonia-oxidizing bacteria using pyruvate kinase genes as high resolution phylogenetic markers2006

    • Author(s)
      Takeshi Ida, Reiji Takahashi, Kazuo Satoh, Mina Kogure, Tatsuaki Tokuyama
    • Journal Title

      Soil Science and Plant Nutrition 52

      Pages: 284-290

    • NAID

      110006251030

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Phylogenetic relationships among ammonia-oxidizing bacteria as revealed by gene sequences of glyceraldehyde 3-phosphate dehydrogenase and phosphoglycerate kinase2005

    • Author(s)
      Takeshi Ida, Michitomo Kugimiya, Mina Kogure, Reiji Takahashi, Tatsuaki Tokuyama
    • Journal Title

      Journal of Bioscience and Bioengineering 99

      Pages: 569-576

    • NAID

      110002695703

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Phylogenetic relationshipa smong ammonia-oxidizing bacteria as revealed by gene sequences of glyceraldehyde 3-phosphate dehydrogenease and phosphoglycerate kinase2005

    • Author(s)
      Takeshi Ida, Michimoto Kigimiya, Mina Kogure, Reiji Takahashi, Tatsuaki Tokuyama
    • Journal Title

      Journal of Bioscience and Bioengineering 99(6)

      Pages: 569-576

    • Related Report
      2005 Annual Research Report
  • [Book] 微生物の世界2007

    • Author(s)
      宮道慎二他編集(共著)
    • Total Pages
      213
    • Publisher
      筑波出版会
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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