Bioorganic Study on Mycoparasitism on Apple Fruit

• Project/Area Number: 17580090
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Hirosaki University
• Principal Investigator: HASHIMOTO Masaru Hirosaki University, Faculty of Agriculture and Life science, Professor, 農学生命科学部, 教授 (40212138)
• Project Period (FY): 2005 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥3,500,000 (Direct Cost: ¥3,500,000); Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2005: ¥2,400,000 (Direct Cost: ¥2,400,000)
• Keywords: mycoparasitism / Lambertella sp. 1346 / Monilinia fructigena / lambertellol / mechanism / responsible substances / acidity of media / culturing conditions / 分子メカニズム / 同位体ラベル / 取り込み率 / 生合成 / マススペクトル / neolamberellin

Research Abstract

The filamentous fungus, Lambertella corni-maris and Lambertella sp. 1346 develop a mycoparasitism on apple fruit against another fungus Monilinia fructigena. Since Lambertella sp.1346 produces lambertellols in substantial amounts and these metabolites induced remarkable antifungal activity against the host Monilinia fructigena, these were expected to be responsible substances for mycoparasitism. On the other hand, Lambertella corni-maris produces these antibiotics but in only a trace of amount under the same conditions employed for Lambertella sp.1346. We found Lambertella corni-maris also provides these antibiotics under acidic conditions. Our investigation also revealed that the host, Monilinia fructigena, changed its surroundings into acidic conditions, suggesting that the acidic conditions acted as kiromons that simulated the productions of them.

Research Products

• [Journal Article] Stimulation of the Biosynthesis of the Antibiotics Lambertellols by the Mycoparasitrc Fungus Lambertella corni-maris under the Acidic Conditions Produced by Its Host Fungus in Vitro (2007)
  • Author(s): T.Murakami, N.Takada, Y.Harada, T.Okuno, M.Hashimoto
  • Journal Title: Biosci. Biotech. Biochem 71(in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Stimulation of the Biosynthesis of the Antibiotics Lambertellols by the Mycoparasitic Fungus Lambertella corni-maris under the Acidic Conditions Produced by Its Host Fungus in Vitro (2007)
  • Author(s): T.Murakami, N.Takada, Y.Harada, T.Okuno, M.Hashimoto
  • Journal Title: Biosci.Biotech.Biochem. (in press)
  • NAID: 10027514666
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Stimulation of the Biosynthesis of the Antibiotics Lambertellols by the Mycoparasitic Fungus Lambertella corni-maris under the Acidic Conditions Produced by Its Host Fungus in Vitro (2007)
  • Author(s): T.Murakami, N.Takada, Y.Harada, T.Okuno, M.Hashimoto
  • Journal Title: Biosci. Biotech. Biochem 71(in press)
  • NAID: 10027514666
• [Journal Article] ピラノース環酸素を硫黄原子で置換したチオオリゴサッカリドの合成研究 (2006)
  • Author(s): 橋本勝
  • Journal Title: 有機合成化学協会誌 64(7) Pages: 766-777
  • NAID: 10017618022
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] An RNA polymerase inhibitor, cyclothiazomycin B1, and its isomer (2006)
  • Author(s): Hashimoto, M., Murakami, T., Funahashi, K., Tokunaga, T., Nihei, K., Okuno, T., Kimura, T., Naoki, H., Himeno, H
  • Journal Title: Bioorg. Med. Chem 14 Pages: 8259-8270
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] An RNA polymerase inhibitor, cyclothiazomycin Bl, and its isomer (2006)
  • Author(s): Hashimoto, M., Murakami, T., Funahashi, K., Tokunaga, T., Nihei, K., Okuno, T., Kimura, T., Naoki, H., Himeno, H.
  • Journal Title: Bioorg.Med.Chem 14 Pages: 8259-8270
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Synthetic Studies on Oligosaccharide Mimics Carrying Sulfur Atoms in the Pyranose Rings (2006)
  • Author(s): Masaru Hashimoto
  • Journal Title: Syn.Org.Chem.Jpn 64 Pages: 766-777
  • NAID: 10017618022
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Isolation, structure elucidation, preparation,and biological properties of neolambertellin (2005)
  • Author(s): Murakami, T., Hashimoto, M., Okuno, T.
  • Journal Title: Bioorg. Med. Chem, Lett. 15 Pages: 4185-4188
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Design, synthesis, and enzymatic property of a sulfur-substituted analogueof trigalacturonic acid (2005)
  • Author(s): Yamamoto, K., Watanabe, N., Matsuda, H., Oohara, K., Araya, T., Hashimoto, M., Miyairi, K., Okazaki, I., Saito, M., Shimizu, T., Kato, H., Okuno, T.
  • Journal Title: Bioorg. Med. Chem, Lett. 15 Pages: 4932-4935
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Synthetic studies on oligosaccharides composed of 5-thioglucopyranose units (2005)
  • Author(s): Morii, Y., Matsuda, Y., Ohara, K., Hashimoto, M., Miyairi, K., Okuno, T.
  • Journal Title: Bioorg. Med. Chem. 13 Pages: 5113-5144
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Lambertellol C, a labile and novel biosynthetic congener of lambertellols A and B (2005)
  • Author(s): Murakami T., Sasaki A., Fukushi E., Kawabata J., Hashimoto M., Okuno T.
  • Journal Title: Bioorg. Med. Chem. Lett. 15 Pages: 2587-2590
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Optimization of isotope-labeling conditions for lambertellin based on isotope patterns observed by mass spectrometry (2005)
  • Author(s): Murakami T., Sasaki A., Fukushi E., Kawabata J., Hashimoto M., Okuno T.
  • Journal Title: Bioorg. Med. Chem. Lett., 15 15 Pages: 2591-2594
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Pachyelasides A-D, Novel Molluscicidal Triterpene Saponins from Pachyelasma Tessmannii (2005)
  • Author(s): Nihei K., YING B.-P., Murakami T., Matsuda H., Hashimoto M., Kubo I.
  • Journal Title: J. Agric. Food Chem. 56 Pages: 608-613
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Design, synthesis, and enzymatic property of a sulfur-substituted analogue of trigalacturonic acid (2005)
  • Author(s): Yamamoto, K., Watanabe, N., Matsuda, H., Oohara, K., Araya, T., Hashimoto, M., Miyairi, K., Okazaki, I., Saito, M., Shimizu, T., Kato, H., Okuno, T.
  • Journal Title: Bioorg.Med.Chem, Lett. 15,22 Pages: 4932-4935
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Isolation, structure elucidation, preparation, and biological properties of neolambertellin (2005)
  • Author(s): Murakami, T., Hashimoto, M., Okuno, T.
  • Journal Title: Bioorg.Med.Chem, Lett. 15 Pages: 4185-4188
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Synthetic studies on oligosaccharides composed of 5-thioglucopvranose units (2005)
  • Author(s): Morii, Y., Matsuda, Y., Ohara, K., Hashimoto, M., Miyairi, K., Okuno, T.
  • Journal Title: Bioorg.Med.Chem. 13 Pages: 5113-5144
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Lambertellol C, a labile and novel biosynthetic congener of lambertellols A and B (2005)
  • Author(s): Murakami T., Sasaki A., Fukushi E., Kawabata J., Hashimoto M., Okuno T.
  • Journal Title: Bioorg.Med.Chem.Lett. 15 Pages: 2587-2590
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Optimization of isotope-labeling conditions for lambertellin based on isotope patterns observed by mass spectrometry (2005)
  • Author(s): Murakami T., Sasaki A.., Fukushi E., Kawabata J., Hashimoto M., Okuno T.
  • Journal Title: Bioorg.Med.Chem.Lett. 15 Pages: 2591-2594
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Pachyelasides A-D, Novel Molluscicidal Triterpene Saponins from Pachyelasma Tessmannii (2005)
  • Author(s): Nihei K., YING B.-P., Murakami T., Matsuda H., Hashimoto M., Kubo I.
  • Journal Title: J.Agric.FoodChem. 53 Pages: 608-613
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Design, synthesis, and enzymatic property of a sulfur-substituted analogueof trigalacturonic acid (2005)
  • Author(s): Yamamoto, K., Watanabe, N., Matsuda, H., Oohara, K., Araya, T., Hashimoto, M., Miyairi, K., Okazaki, I., Saito, M., Shimizu, T., Kato, H., Okuno, T.
  • Journal Title: Bioorg.Med.Chem, Lett., 15 Pages: 4932-4935
• [Journal Article] Isolation, structure elucidation, preparation, and biological properties of neolambertellin (2005)
  • Author(s): Murakami, T., Hashimoto, M., Okuno, T.
  • Journal Title: Bioorg.Med.Chem, Lett., 15 Pages: 4185-4188
• [Journal Article] Synthetic studies on oligosaccharides composed of 5-thioglucopyranose units (2005)
  • Author(s): Morii, Y., Matsuda, Y., Ohara, K., Hashimoto, M., Miyairi, K., Okuno, T.
  • Journal Title: Bioorg.Med.Chem. 13 Pages: 5113-5144
• [Journal Article] Optimization of isotope-labeling conditions for lambertellin based on isotope patterns observed by mass spectrometry (2005)
  • Author(s): Murakami T., Sasaki A., Fukushi E., Kawabata J., Hashimoto M., Okuno T.
  • Journal Title: Bioorg.Med.Chem.Lett. 15 Pages: 2591-2594
• [Journal Article] Pachyelasides A-D, Novel Molluscicidal Triterpene Saponins from Pachyelasma Tessmannii (2005)
  • Author(s): Nihei K, YING B.-P., Murakami T., Matsuda H., Hashimoto M., Kubo I.
  • Journal Title: Agric.Food Chem. 53 Pages: 608-613
• [Patent(Industrial Property Rights)] 新規環状チオペプチドおよびその取得方法 (2006)
  • Inventor(s): 橋本勝, 姫野俵太, 村上貴宣, 奥野智旦
  • Industrial Property Rights Holder: 弘前大学
  • Industrial Property Number: 2006-058680
  • Filing Date: 2006-03-03
  • Description: 「研究成果報告書概要(和文)」より