| Project/Area Number |
17580096
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Kobe University |
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Principal Investigator |
SUGIMOTO Yukihiro Kobe University, Faculty of Agriculture, Professor, 農学部, 教授 (10243411)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAUCHI Yasuo Kobe University, Faculty of Agriculture, Assistant Professor, 農学部, 助手 (90283978)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | root parasitic plant / Orobanche / Striga / host plant / Lotus japonicus / interaction / ソルガム |
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| Research Abstract |
Root parasitic plants directly invade and rob water and nutrients from host plants and thus cause major constraints to crop production in many parts of the world. Lotus japonicus, a model leguminous plant, is compatible to the holoparasite Orobanche aegyptiaca, but incompatible to the hemiparasite Striga hermonthica. In order to understand host responses at early stages of parasitization at a molecular level, we established cDNA libraries enriched by suppression subtractive hybridization (SSH) for transcripts regulated in L. japonicus root segments adjacent to O. aegyptiaca tubercles (Oa), and adjacent to the attachment points of O. minor (Om) and S. hermonthica seedlings (Sh). Following sequencing and expression analyses, we found that 59, 21 and 46 genes were up-regulated in the Oa, Om and Sh libraries, respectively. These libraries were enriched for transcripts predicted to function in stress responses including PR proteins and enzymes involved in jasmonic acid biosynthesis. Transcripts associated with nodulation were significantly more abundant in the Oa library, while those associated with phytoalexin biosynthesis were found exclusively in the Sh library. These results suggest that the compatible parasitic plant deceives the host plant into lowering resistance at early stages of parasitization.
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