Live cell Imaging of marine Bacterial Gene Expression(LIBAGE)
| Project/Area Number |
17580156
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | The University of Tokyo |
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Principal Investigator |
WADA Minoru The University of Tokyo, Ocean Research Institute, Research Associate, 海洋研究所, 助手 (70292860)
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| Co-Investigator(Kenkyū-buntansha) |
KARATANI Hajime Kyoto Institute of Technology, Professor, 繊維学部, 教授 (10169659)
KITA Kumiko (TSUKAMOTO Kumiko) The University of Tokyo, Ocean Research Institute, Chief Technician, 海洋研究所, 技術専門職員 (30396923)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | lux gene / GFP / Photobacterium leiognathi / 緑膿菌 / バイオフィルム |
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| Research Abstract |
In this project, we aimed to clarify the bacterial-animal interaction in marine environments by using molecular techniques. A part of the achievements during the project is summarized below.
1: We developed a simple and reliable method to identify marine bioluminescent bacteria based on the amplified ribosomal DNA restriction analysis(ARDRA).
2: We found that the luciferase genes of a symbiotic luminescent bacterium, Photobacterium leiognathi, formed two distinct lineages depending on the host organisms.
3: We established a quantitative PCR method to estimate abundance of P.leiognathi in marine environments.
4: We constructed green fluorescent protein(GFP)-tagged cells of P.leiognathi which allowed us to monitor both the fluorescence and the luminescence from the cells.
5: We found that the GFP-tagged P.leiognathi cells colonized over the mucoid material secreted from a polychaete, Capitella sp.I, when the bacterium and the worms were kept together in a sediment microcosm.
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Report
(3 results)
Research Products
(6 results)
