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Neuropathology of brain myxosporean disease of fish

Research Project

Project/Area Number 17580157
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionTHE UNIVERSITY OF TOKYO

Principal Investigator

YOKOYAMA Hiroshi  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Assistant, 大学院農学生命科学研究科, 助手 (70261956)

Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsmyxosporean / spinal curvature / Myxobolus / neuropathology / aquaculture / brain / 脊椎彎曲
Research Abstract

1.Pathological analysis of skeletal deformity of cultured fish : Diseased Japanese mackerel (Scomber japonicus) exhibited dorso-ventral curvature externally. Histopathological examination revealed that the spinal curvature was caused by a brain myxosporean, Myxobolus acanthogobii. Myxosporean cysts were found in the olfactory lobe, optic lobe, the cavity of optic tectum and the fourth ventricle. However, the anatomical location of cysts responsible for the skeletal abnormalities could not be determined.
2.Pathological analysis of naturally infected but subclinical wild fishes : Brown-lined puffer (Canthigaster rivulata) and yellowfin goby (Acanthogobius flavimanus) were found to be naturally infected with M.acanthogobii, but did not show any skeletal abnormalities. Histopathological observation showed that numerous cysts were found in the optic lobe, medulla oblongata and cerebellum.
3.Development of PCR-based detection method for M.acanthogobii-A PCR method targeting SSU rDNA of M.acanthogobii was developed. Nested-PCR analysis was applied to epizootiological survey of the parasite in feral fishes (37 species). As a result, forksnout searobin (Lepidotrigla alata) and crimson seabream (Evynnis japonica) were found to be the hosts for M.acanthogobii. Further, surveys on possible alternate annelid hosts for M.acanthogobii were conducted, but actinosporeans and PCR-positive reactions were not obtained.
4.Experimentally induced abnormal behavior of fish caused by implant by brain surgery : Glass beads (ca.1 mm in diameter) were implanted in the 4th ventricle of goldfish, and swimming behavior was observed. As a result, the experimental fish showed a notable body curvature and whirling behavior. This suggests that spinal curvature is caused by mechanical pressure of foreign particles localized in the 4th ventricle of fish brain.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (6 results)

All 2005

All Journal Article (6 results)

  • [Journal Article] Spinal curvature of cultured Japanese mackerel Scomber japonicus associated with a brain myxosporean, Myxobolus acanthogobii2005

    • Author(s)
      Yokoyama H., M.A.Freeman, N Itoh, Y.Fukuda
    • Journal Title

      Diseases of Aquatic Organisms 66・1

      Pages: 1-7

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A PCR method to detect Myxobolus acanthogobii (Myxozoa : Myxosporea), the causative agent of skeletal deformities of marine fishes.2005

    • Author(s)
      Miyajima S., H.Yokoyama, Y.Fukuda, K.Okamoto, K.Ogawa
    • Journal Title

      Fish Pathology 40・4

      Pages: 197-199

    • NAID

      10019225554

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Spinal curvature of cultured Japanese mackerel Scomber japonicus associated with a brain myxosporean, Myxobolus acanthogobii2005

    • Author(s)
      Yokoyama H. et al.
    • Journal Title

      Disease of Aquatic Organisms 66(1)

      Pages: 1-7

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A PCR method to detect Myxobolus acanthogobii (Myxozoa : Myxosporea), the causative agent of skeletal deformities of marine fishes2005

    • Author(s)
      Miyajima S. et al.
    • Journal Title

      Fish Pathology 40(4)

      Pages: 197-199

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A PCR method to detect Myxobolus acanthogobii (Myxozoa : Myxosporea), the causative agent of skeletal deformities of marine fishes2005

    • Author(s)
      Miyajima S, Yokoyama H, Fukuda Y, Okamoto K, Ogawa K
    • Journal Title

      Fish Pathology 40(4)

      Pages: 197-199

    • NAID

      10019225554

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Spinal curvature of cultured Japanese mackerel Scomber japonicus associated with a brain myxosporean, Myxobolus acanthogobii2005

    • Author(s)
      Yokoyama H, Freeman MA, Itoh N, Fukuda Y
    • Journal Title

      Diseases of Aquatic Organisms 66(1)

      Pages: 1-7

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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