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Development of needle-type biosensor system for monitoring of fish health

Research Project

Project/Area Number 17580180
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionTokyo University of Marine Science and Technology

Principal Investigator

ENDO Hideaki  Tokyo University of Marine Science and Technology, Faculty of Marine Science, Associate professor, 海洋科学部, 助教授 (50242326)

Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsBiosensor / Enzyme sensor / Blood / Glucose / Fish / Culture / Medical examination / Needle / バイオセンサ / 酵素センサ / コレステロール / 血漿 / シリコーン
Research Abstract

Mass outbreaks of pathogenic bacterial infections have killed large quantities of fish in recent years, causing great financial damage to fish farms. Periodic checks of fish health and detection of abnormalities as quickly as possible are thus necessary at fish farms. Several studies have shown blood glucose levels closely correlate to the level of stress in fish and represent the state of respiratory or nutritional disturbance. Recent studies have also clarified that decreased levels of blood cholesterol represent a useful indicator of reduced resistance to bacterial infection. In this manner, determination of blood glucose and cholesterol is important for ascertaining the health of fish. A needle-type biosensor system was developed for rapid and simple determination of glucose levels in fish blood. The sensor comprises a needle-type hollow container (18-gauge needle), immobilized enzyme membrane and optic fiber probe with ruthenium complex. The enzyme membrane was prepared from glucose oxidase, azide-unit pendant water-soluble photopolymer and an ultra-thin dialysis membrane. The optic fiber probe was inserted into the rolled enzyme membrane placed in the needle-type hollow container. The calibration curve was linear for glucose levels in fish plasma. One assay was completed within 3 min. A good reproducibility was observed for 60 times without exchange of the enzyme membrane. The sensor was inserted into the caudal vein of fish to measure blood glucose levels. The sensor responded immediately after insertion and glucose levels could be monitored. Good correlations were observed between values determined using the sensor and conventional methods in the range of 48-157 mg d1-1 (correlation coefficient, 0.9474).

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (7 results)

All 2006 2005

All Journal Article (2 results) Book (3 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] A needle-type optical enzyme sensor system for determining glucose levels in fish blood2006

    • Author(s)
      H.Endo, Y.Yonemori, K.Musiya, M.Maita, T.Shibuya, H.Ren, T.Hayashi, K.Mitsubayashi
    • Journal Title

      Anal. Chim. Acta 573, 574

      Pages: 117-124

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] A needle-type optical enzyme sensor system for determining glucose levels in fish blood.2006

    • Author(s)
      H.Endo, Y.Yonemori, K.Musiya, M.Maita, T.Shibuya, H.Ren, T.Hayashi, K.Mitsubayashi
    • Journal Title

      Anal.Chim.Acta 573, 574

      Pages: 117-124

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] ユビキタス・バイオセンシング : 第4編第4章 : 食としての魚の安全性をセンシングする2006

    • Author(s)
      遠藤英明(監修 三林浩二)
    • Publisher
      シーエムシー出版
    • Related Report
      2006 Annual Research Report
  • [Book] ユビキタス・バイオセンシング : 第4編第4章 : 食としての魚の安全性をセンシングする2005

    • Author(s)
      遠藤英明, (監修 三林浩二)
    • Publisher
      シーエムシー出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 「未来を拓くユビキタス・バイオセンシング」食としての魚の安全性をセンシングする(分筆,P247-259)2005

    • Author(s)
      遠藤英明(三林浩二編)
    • Total Pages
      291
    • Publisher
      シー・エム.シー出版
    • Related Report
      2005 Annual Research Report
  • [Patent(Industrial Property Rights)] バイオセンサーとその製造方法2006

    • Inventor(s)
      遠藤英明
    • Industrial Property Rights Holder
      科学技術振興機構
    • Filing Date
      2006-02-10
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Patent(Industrial Property Rights)] バイオセンサーとその製造方法2005

    • Inventor(s)
      遠藤英明
    • Industrial Property Rights Holder
      東京海洋大学
    • Filing Date
      2005-02-24
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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