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Analysis of Genes Encoding the Enzymes Degrading Nonylphenol Compounds which Exert Endocrine Disrupting Activity

Research Project

Project/Area Number 17580185
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionNational Fisheries University

Principal Investigator

SHIBA Tsuneo  National Fisheries University, Department of Food Science and Technology, Professor, 食品化学科, 教授 (20126080)

Co-Investigator(Kenkyū-buntansha) MAEDA Toshimichi  National Fisheries University, Department of Food Science and Technology, Lecturer, 食品化学科, 講師 (20399653)
FURUSHITA Manabu  National Fisheries University, Department of Food Science and Technology, Assistant Professor, 食品化学科, 助手 (40399655)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsnonylphenol / bacteria / degradation / Marinobacter
Research Abstract

The strain Marinobacter sp.TY4, which was isolated from coastal sea sand, exerted degradation activity of nonylphenol oligoethoxylates. The activity is expressed by only the cells harvested at stationary growth phase, and only in the presence of particles which adsorb the nonylphenols Shortening of the ethoxylate chains was not observed, and the benzene ring seemed to be directly cleaved
The gene cluster, being engaged in the degradation of benzene compounds, were found in the cell and comprised of catBCA, benABCDKE. Mutants defected with catA or benA were developed a suicide vector. The defected mutants still exerted the degradation activity, while the catechol degradation activity was halved. Another degradation enzyme was suggested to be present.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (3 results)

All 2005

All Journal Article (3 results)

  • [Journal Article] Characteristics of nonylphenol polyethoxylate- degrading bacteria isolated from coastal sediments.2005

    • Author(s)
      T.Maeda, K.Hayakawa, M.You, M.Sasaki, Y.Yamaji, M.Furushita, T.Shiba
    • Journal Title

      Microbes and Environments 20

      Pages: 253-257

    • NAID

      110002978283

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Characteristics of nonylphenol polyethoxylate-degrading bacteria isolated from coastal sediments.2005

    • Author(s)
      T.Maeda, K.Hayakawa, M.You, M.Sasaki, Y.Yamaji, M.Furushita, T.Shiba
    • Journal Title

      Microbes and Environment. 20

      Pages: 253-257

    • NAID

      110002978283

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Characteristics of nonylphenol polyethoxylate-degrading bacteria isolated from coastal sediments.2005

    • Author(s)
      T.Maeda, K.Hayakawa, M.You, M.Sasaki, Y.Yamaji, M.Furushita, T.Shiba
    • Journal Title

      Microbes and Environments 20

      Pages: 253-257

    • NAID

      110002978283

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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