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Establishment for the use of supplements for reducing methane and increasing fiber digestion in the rumen

Research Project

Project/Area Number 17580234
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

ITABASHI Hisao  Tokyo University of Agriculture and Technology, Faculty of Agriculture, Professor (00280991)

Co-Investigator(Kenkyū-buntansha) KUROKAWA Yuzo  Tokyo University of Agriculture and Technology, Faculty of Agriculture, Associate Professor (00234592)
TAJIAM Kiyoshi  National Institute of Livestock and Grassland Science, Department of Animal Physiology and Nutrition, Researcher (80343953)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥2,600,000 (Direct Cost: ¥2,600,000)
KeywordsRumen / Fiber digestion / Probiotics / Methane / Volatile fatty acid / Rumen protozoa / Rumen bacteria / Cellobiose / ウシ / プロトゾア / プロピオン酸
Research Abstract

Effects of feed additives on methane production and fiber digestion in the rumen were studies by in vitro incubation and in vivo experiment. Addition of fumaric acid, saponins and plant extracts (eucaryptus-oil, horseradish-oil) decreased methane production by 10-60%. In the case of plant extracts, hydrogen production was increased, but simultaneous addition of fumaric acid decreased its production. The addition of probiotics (Saccaromyces cerevisiae) and cellobiose increased fiber digestion by 10%.
Effect of protozoa on ruminal bacteria of cattle was also studied by molecular techniques. Among methanogens, Methanobacteriales was predominant in all cattle studied, and one clone was detected only from protozoa-free cattle. The number of Ruminococcus albus and R.flavofaciens were higher in protozoa-free cattle, but that of Fibrobacter sccinogenes were higher in faunated cattle, indicating protozoa have a significant role in ruminal fiber digestion.
In conclusion, combination use of fumaric acid, saponins, and herb could decrease methane production, without decrease in protozoal number and fiber digestion, and increase of hydrogen production. And, the addition of cellobiose and probiotics could increase ruminal fiber digestion effectively, and could contribute to increase fiber utilization in ruminants.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (18 results)

All 2008 2007 2006 2005

All Journal Article (17 results) Book (1 results)

  • [Journal Article] Effects of the essential oil-cyclodextrin ruminal methane production in vitro2008

    • Author(s)
      Tatsuoka, N., Koji Hara, K. Mikuni, K.Hara, H. Hashimoto, H. Itabashi
    • Journal Title

      Animal Science Journal 79(In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of the essential oil-cyclodextrin complexes on ruminal methane production in vitro2008

    • Author(s)
      Tatsuoka, N, Koji Hara, K. Mikupi, K. Hara, H. Hashimoto and H. Itabashi
    • Journal Title

      Animal Science Journal 79(In press)

    • NAID

      10025368638

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of protozoa on the antioxidant activity in the ruminal fluid and blood plasma of cattle2007

    • Author(s)
      Masudur R. GAZI, Masato YOKOTA, Yuichi TANAKA, Shuhei KANDA, Hisao ITABASHI
    • Journal Title

      Animal Science Journal 78

      Pages: 34-40

    • NAID

      10021224105

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Analysis of methanogens in the bovine rumen by polymerase chain reaction single-strand conformation polymorphism2007

    • Author(s)
      Tatsuoka, N., N. Mohammed, M. Mitsumori, K. Tajima, K. Hara, M. Kurihara, H. Itabashi
    • Journal Title

      Animal Science Journal 78

      Pages: 512-518

    • NAID

      10021226323

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Analysis of methanogens in the bovine rumen by pol'merase chain reaction single-strand conformation polimorphism2007

    • Author(s)
      Tatsuoka, N, N. Mohammed, M.Mitsumori, K. Tajima, K. Hara, M. Kurihara, H. Itabashi
    • Journal Title

      Animal Science Journal 78

      Pages: 512-518

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of protozoa on the antioxidant activity in the ruminal fluid and blood plasma of cattle2007

    • Author(s)
      Masudur R.GAZI, Masato YOKOTA, Yuichi TANAKA, Shuhei KANDA, Hisao ITABASHI
    • Journal Title

      Animal Science Journal 78

      Pages: 34-40

    • NAID

      10021224105

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effects of the essential oil-cyclodextrin complexes on ruminal methane production in vitro2007

    • Author(s)
      Noriko Tatsuoka, Koji Hara, Katsuhiko Mikuni, Kozo Hara, Hitoshi Hashimoto, Hisao Itabashi
    • Journal Title

      Animal Science Journal 78 (In press)

    • NAID

      10025368638

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effect of Presence of Rumen Protozoa on Degradation of Cell Wall Constituents in Gastrointestinal Tract of Cattle2007

    • Author(s)
      Natsuki KASUYA, Itsuko WADA, Mimune SHIMADA, Hiroshi KAWAI, Hisao ITABASHI
    • Journal Title

      Animal Science Journal 78 (In press)

    • NAID

      10021225216

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Increase of ruminal fiber digestion by cellobiose and atwin-strain of Saccharomyces cerevisiae live cells in vitro2006

    • Author(s)
      Lila, Z. A., N. Mohammed, T. Takahashi, M. Tabata, T. Yasui, Kurihara, S. Kanda, H. Itabashi
    • Journal Title

      Animal Science Journal 77

      Pages: 407-413

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Real-time PCR detection ofthe effects of protozoa on rumenbacteria in cattle2006

    • Author(s)
      Yuhei Ozutsumi, Kiyoshi Tajima, Akio Takenaka, Hisao Itabashi
    • Journal Title

      Current Microbiology 52

      Pages: 158-162

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Increase of ruminal fiber digestion by cellobiose and a twin-strain of Saccharoniyces cerevisiae live cells in vitro.2006

    • Author(s)
      Lila, Z. A., N. Mohammed, T. Takahashi, M. Tabata, T. Yasui, Kurihara, S. Kanda, H. Itabashi
    • Journal Title

      Animal Science Journal 77

      Pages: 407-413

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Real-time PCR detection of the effects of protozoa on rumen bacteria in cattle.2006

    • Author(s)
      Yuliei Ozutsumi, Kiyoshi Tajinia, Akio Takenaka, Hisao Itabashi.
    • Journal Title

      Current Microbiology 52

      Pages: 158-162

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Increase of ruminal fiber digestion by cellobiose and a twin-strain of Saccharomyces cerevisiae live cells in vitro.2006

    • Author(s)
      Lila, Z.A., N.Mohammed, T.Takahashi, M.Tabata, T.Yasui, M.Kurihara, S.Kanda, H.Itabashi
    • Journal Title

      Animal Science Journal 77

      Pages: 407-413

    • NAID

      10021222889

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Culture-independent analysis of fecal microbiota in cattle.2005

    • Author(s)
      Y.Ozutsumi, H.Hayashi M.Sakamoto, H, Itabashi, Y.Benno
    • Journal Title

      Biosci.Biotechnol.Biochem. 69

      Pages: 1793-1797

    • NAID

      130000030223

    • Related Report
      2005 Annual Research Report
  • [Journal Article] ルーメンプロトゾアの繊維分解酵素とその遺伝子解析2005

    • Author(s)
      板橋久雄, 竹中昭雄
    • Journal Title

      栄養生理研究会報 49

      Pages: 65-79

    • NAID

      10015561951

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The effect of protozoa on the composition of rumen bacteria in cattle using 16SrRNA gene clone libraries2005

    • Author(s)
      Y.Ozutsumi, K.Tajima, A.Takenaka, H.Itabashi
    • Journal Title

      Biosci.Biotechnol.Biochem. 69

      Pages: 499-506

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Real time PCR detecyion of the effects of protozoa on rumen bacteria in cattle2005

    • Author(s)
      Y.Ozutsumi, K.Tajima, A.Takenaka, H.Itabashi
    • Journal Title

      Current Microbiology 51

      Pages: 1-6

    • Related Report
      2005 Annual Research Report
  • [Book] ルミノロジーの基礎と応用2006

    • Author(s)
      板橋 久雄(共著)
    • Total Pages
      301
    • Publisher
      農文協
    • Related Report
      2006 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

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