PCR-DGGE analysis of silage microorganisms and its application to food quality control
| Project/Area Number |
17580236
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Zootechnical science/Grassland science
|
|---|
| Research Institution | Okayama University |
|---|
Principal Investigator |
NAOKI Nishino Department of Animal Science, Associate Professor, 大学院自然科学研究科, 助教授 (50237715)
|
|---|
| Project Period (FY) |
2005 – 2006
|
| Project Status |
Completed (Fiscal Year 2006)
|
| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,300,000 (Direct Cost: ¥2,300,000)
|
|---|
| Keywords | Feed / Microbiology / PCR-DGGE / Silage |
|---|
| Research Abstract |
It has become a common practice in Japan that high-moisture by-products are stored with dry feeds as an total mixed ration silage. This may minimize the risk of effluent production and can omit the time for mixing prior to feeding. Unpalatable by-products could be incorporated into a TMR silage, if their odors and flavors are altered through the fermentation in a silo. An interesting property of the TMR silage is high stability on exposure to air. Heating would not occur even in summer, whereas non-ensiled TMR mixture may deteriorate quite rapidly. Conventional criteria do not account well for the stability, because the TMR silage can remain unheated even with a large number of yeasts and a substantial level of substrates (sugars and lactic acid) for the growth of aerobic microorganisms. We have investigated the factors involved in resistance to deterioration by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), and some interesting results are obtained as fo
… More
llows.
1. When wet brewers grains and soybean-curd refuse were stored alone or in combination with other feeds as a TMR silage, deterioration did not occur only in TMR silages. Without anaerobic fermentation, TMR mixture also spoiled quite easily.
2. Lactobacillus buchneri, a hetero-fermentative lactic acid bacteria which is capable of enhancing aerobic stability, was not found in the silage material, but appeared from the early ensiling period in TMR silage containing wet brewers grains. Although aerobic stability was similarly high in TMR silage containing soybean-curd refuse, L. buchneri was not detected regardless of ensiling period.
3. Weisella sp. and Lactobacillus brevis was found in both the stable TMR silages. Unstable by-products silage stored alone did not contain these two lactic acid bacteria.
4. We could isolate those L. buchneri, L. brevis and Weisella confusa from the following experiment, hence now are planning to inoculation experiment to determine if these bacterial mixture can actually improve the aerobic stability of silage. Less
|
Report
(3 results)
Research Products
(4 results)
