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A novel rice accumulating fructan in the vacuole as a temporary storage carbohydrate and its evaluation for forage

Research Project

Project/Area Number 17580241
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionNational Agricultural Research Organization

Principal Investigator

YOSHIDA Midori  National Agricultural Research Organization, National Agricultural Research Center for Hokkaido Region, Senior Researcher (00355455)

Co-Investigator(Kenkyū-buntansha) YAMADA Toshihiko  Hokkaido University, 北方生物圏フィールド科学センター, Professor (70343952)
KAWAKAMI Akira  National Agriculture and Food Research Organization, National Agricultural Research Center for Kyushu Okinawa Region, Senior Researcher (00370601)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsfructan / rice / forage / 試料イネ / 飼料イネ
Research Abstract

The feasibility of using rice of forage as whole crop silage has been recently investigated in Japan because of the decrease in self-sufficiency of domestic forage and the over-production of rice. Fructan is a water-soluble oligo- and polysaccharide. Temperate grasses accumulate fructan in their vacuoles as a temporary storage carbohydrate, whereas rice is not able to synthesize fructan. It has been reported that fructan improves the rumen condition as well as the digestibility. In order to increase the quality of rice forage, we introduced wheat fructosyltransferase genes into rice and succeeded in obtaining transgenic rice plants that accumulate fructan in tissues such as the leaves and stems. A rice transformant with 1-SST (sucrose: sucrose 1-fructosyltransferase) gene accumulates β-(2, 1) linkage oligomer (DP3-5), and a rice transformant with 6-SFT (sucrose: fructan 6-fructosyltransferase) gene accumulates mainly β-(2, 6) linkage fructan. A rice plant carrying both 1-SST and 6-SFT genes showed an additional accumulation of fructan with β-(2, 6) linked fructosyl-units attached to bifurcose. When the plants were grown in a greenhouse under summer light conditions, fructan contents in leaves of the transgenic lines with wheat 1-SST and with both two genes reached about 20 mg and 30 mg/g fresh weight, respectively. The content of total soluble sugar also increased in these lines. On the other hand, fructan accumulation levels in the transgenic lines with wheat 6-SFT gene were low (2-7 mg/g fresh weight). Moreover, most of the transgenic lines showed no morphological abnormalities. This fructan-accumulating rice has the potential for utilization for forage. Investigation will be continued.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (5 results)

All 2007 2006 2005

All Journal Article (1 results) Book (1 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Graminai Metabolism in Cereals : Wheat as a Model System2007

    • Author(s)
      Yoshicia M., Kawakami K., Van den Ende W
    • Journal Title

      In Recent Advances in Fructool igosacchar ides Research, ByEds. Shiomi N., Benkeblia, N. and Onodera, S. Chapter 10

      Pages: 201-212

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] Graminan Metabolism in Cereals:Wheat as a Model System.In Recent Advances in Fructooligosaccharides Research.Chapter 10.By Eds.Shiomi N., Benkeblia, N.and Onodera, S.,2007

    • Author(s)
      Yoshida M., Kawakami K., Van den, Ende W
    • Publisher
      Research Signpost Publisher
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 耐冷性イネとその開発力法2006

    • Inventor(s)
      吉田 みどり、川上 顕、佐藤 裕
    • Industrial Property Rights Holder
      (独)農業・食品産業技術総合研究機構
    • Filing Date
      2006-06-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 耐冷性イネとその開発方法2006

    • Inventor(s)
      吉田みどり, 川上顕, 佐藤裕
    • Industrial Property Rights Holder
      (独)農業・食品産業技術総合研究機構
    • Filing Date
      2006-06-21
    • Related Report
      2006 Annual Research Report
  • [Patent(Industrial Property Rights)] 耐冷性植物及びその開発方法2005

    • Inventor(s)
      吉田 みどり, 川上 顕, 佐藤 裕
    • Industrial Property Rights Holder
      (独)農業・生物系特定産業技術研究機構
    • Industrial Property Number
      2005-182251
    • Filing Date
      2005-06-22
    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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