| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Research Abstract |
The full amino acid coding sequences of adrenergic receptor beta1, beta2, and beta3 (ADRB1, ADRB2, and ADRB3) genes were determined from Meishan, Duroc, and Landrace pigs. Non-synonymous substitution of Arg458Pro was found in the pig ADRB1 gene coding a 469 amino acids sequence. Continuous substitutions of Asn29Asp and Glu30Gln were found in the pig ADRB2 gene coding a 418 amino acid sequence. There were three non-synonymous substitutions of Glu24Thr, Arg264Glu and Glu398Asp in the pig ADRB3 gene. A thymine insertion in ADRB3 gene resulting in two amino acids shorter protein was found in Meishan pigs.
To assess the effects of ADRB variants on porcine subcutaneous fat layer thickness, genetic frequency of the variants were calculated in fatty and lean groups, consisted of each 24 animals of the crossbred of Duroc and Jinhua pigs. There was no sufficient variation in the ADRB3 gene, and x^2 test showed no significant difference in the ADRB1 and ADBR2 genes of the two groups. However, Arg458 homozygotes of ADRB1 were higher in lean group and had a little thin fat layer than Pro458 variant. The Asp29-Lys30 genotypes of ADRB2 were higher in lean group, and theAsn29-Glu30 genotypes were higher in fatty group. These results suggested that ADRB polymorphism is a minor factor for porcine subcutaneous fat layer thickness.
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