GENERATION OF TRANSGENIC MICE EXPRESSING SOLUBLE FORM OF PORCINE NECTIN-1 AS A MODEL FOR PSEUDORABIES-RESISTANT LIVE STOCK
| Project/Area Number |
17580265
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | TOTTORI UNIVERSITY |
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Principal Investigator |
ONO Etsuro Tottori University, Faculty of Agriculture, Professor, 農学部, 教授 (00160903)
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| Co-Investigator(Kenkyū-buntansha) |
TOMIOKA Yukiko Hokkaido University, Institute of Genetic Medicine, Instructor, 遺伝子病制御研究所, 助手 (50374674)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Disease-resistant animal / Aujeszky's disease / Pseudorabies / Nectin / Transgenic mouse / Herpesvirus |
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| Research Abstract |
Nectin-1, also known as herpesvirus entry mediator C (HveC), is an alphaherpesvirus receptor that binds to virion glycoprotein D (gD) by the first immunoglobulin (Ig)-like domain. We generated three each transgenic mouse lines expressing a fusion protein (VpIg) consisting of the first Ig-like domain of porcine nectin-1 and the Fc portion of porcine IgG1 or a fusion protein (VCCpIg) consisting of the entire ectodomain of porcine nectin-1 and the Fc portion of porcine IgG1 to compare the protective effects induced by the expression of VCChIg, VpIg and VCCpIg in the transgenic mice. Three different transgenic mouse lines were challenged with 20 LD_<50>, 100 LD_50 or 1000 LD_<50> of PRV strainYS-81 via intraperitoneal route. In the higher virus doses, significantly stronger protections were induced by the expression of VCChIg and VCCpIg as compared with those by VpIg. In the intranasal challenge with 10 LD_<50> of PRV, relatively strong protection was, taken overall, observed in the transgenic mouse lines expressing VCChIg and VCCpIg as compared with that in the lines expressing VpIg. It was also found that both of VCChIg and VCCpIg possess the almost same antiviral potential, indicating that the VCCpIg gene may be more suitable for the application for generating pseudorabies-resistant livestock in the context of porcine immune system and the issue of possible adverse effects. The present results demonstrate that the second and third Ig-like domains enhance the antiviral effect against pseudorabies virus infection and the Fc portions from different species do not significantly affect the antiviral potential in transgenic mice.
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Report
(3 results)
Research Products
(5 results)
