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Studies on the mechanism of myelin regeneration using mutant rats with myelin disorders

Research Project

Project/Area Number 17580268
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionOsaka Prefecture University

Principal Investigator

KUWAMURA Mitsuru  Osaka Prefecture University, Graduate School of Life & Environmental Sciences, Lecturer (20244668)

Co-Investigator(Kenkyū-buntansha) TAKENAKA Shigeo  Osaka Prefecture University, Graduate School of Life and Environmental Science, Associate Professor (10280067)
YAMEATE Jyoji  Osaka Prefecture University, Graduate School of Life and Environmental Science, Associate Professor (50150115)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsdemvelination / myelin / regeneration / neural stem cell
Research Abstract

The myelin vacuolation (my) rat is an autosomal recessive mutant characterized by hypomyelination and vacuole formation in the myelin throughout the central nervous system (CNS). Previous genetic studies have revealed a null mutation in attractin gene of the my mutant rat. It has been known that mutation at the attractin locus results in myelin alterations, but their detailed pathogenesis is still unclear. In this study, we examined glial changes in the spinal cord of my rats at 2, 4, 6, 8 weeks of age and identified attractin-expressing cells in the rat spinal cord.
No abnormality was found in the number and morphology of oligodendrocytes in my rats at any of the ages examined, although the severity and extent of myelin disorder increased with age. Coincident with the myelin abnormalities, there was progressive astrogliosis from 2 weeks. Marked microglial activation was observed exclusively in the gray matter of my rats from 6 weeks, coincident with severe myelin disruption. A double-labeling study demonstrated that attractin-expressing cells in the spinal cord are mostly oligodendrocytes in wild-type rats, whereas no attractin-positive cells were detected in my rats.
These findings indicate that the attractin defect results in oligodendrocyte dysfunction, and is associated with astrogliosis and microglial activation in my rats. The data suggest that attractin may be directly involved in the function of oligodendrocytes in CNS myelination.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (14 results)

All 2008 2007 2006 2005 Other

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (9 results) Remarks (2 results)

  • [Journal Article] Cellular responses in the spinal cord during development of hypomyelination in the mv rat2008

    • Author(s)
      Izawa T, et. al.(Corresponding author: Kuwamura M)
    • Journal Title

      Brain Research 1195

      Pages: 120-129

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] (Corresponding author: Kuwamura, M) Cellular responses in the spinal cord during development of hypomyelination in the mv rat2008

    • Author(s)
      Izawa, T., et. al.
    • Journal Title

      Brain Research 1195

      Pages: 120-129

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Cellular responses in the spinal cord during development of hypomyelination in the mv rat2008

    • Author(s)
      Izawa T, et. al.(Correspollding author:Kuwamura M)
    • Journal Title

      Brain Research 1195

      Pages: 120-129

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] アトラクチン欠損ミュータントmvラットにおけるミエリン関連遺伝子・蛋白の発現解析2008

    • Author(s)
      井澤 武史, 他
    • Organizer
      第145回 日本獣医学会
    • Place of Presentation
      相模原市
    • Year and Date
      2008-03-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Studies on expression of myelin-related mRNA and protein in the attractin deficient my rat2008

    • Author(s)
      Izawa, T., et. al.
    • Organizer
      145th annual meeting of Japanese Society of Veterinary Science
    • Place of Presentation
      Sagamihara
    • Year and Date
      2008-03-29
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] アトラクチン欠損ミュータントmvラットにおけるミクログリア関連因子の動態2007

    • Author(s)
      井澤 武史, 他
    • Organizer
      第143回 日本獣医学会
    • Place of Presentation
      つくば
    • Year and Date
      2007-04-04
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Kinetics of microglia-related cytokine in the attractin deficient my rat.2007

    • Author(s)
      Izawa, T., et. al.
    • Organizer
      143th annual meeting of Japanese Society of Veterinary Science
    • Place of Presentation
      Tsukuba
    • Year and Date
      2007-04-04
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] アトラクチン欠損ミュータントmvラットにおけるミクログリア関連因子の動態2007

    • Author(s)
      井澤 武史, 他
    • Organizer
      第143回日本獣医学会
    • Place of Presentation
      つくば
    • Year and Date
      2007-04-04
    • Related Report
      2007 Annual Research Report
  • [Presentation] アトラクチン欠損ミュータントmvラットにおけるグリア細胞の役割2006

    • Author(s)
      井澤 武史, 他
    • Organizer
      第141回 日本獣医学会
    • Place of Presentation
      つくば
    • Year and Date
      2006-03-19
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Roles of glial cells in the attractin deficient my rat2006

    • Author(s)
      Izawa, T., et. al.
    • Organizer
      141th annual meeting of Japanese Society of Veterinary Science
    • Place of Presentation
      Tsukuba
    • Year and Date
      2006-03-19
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] アトラクチン欠損ミュータントmvラットにおけるグリア細胞動態2005

    • Author(s)
      井澤 武史, 他
    • Organizer
      第88回 関西実験動物研究会
    • Place of Presentation
      京都
    • Year and Date
      2005-12-02
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Kinetic of glial cells in the attractin deficient my rat2005

    • Author(s)
      Izawa, T., et. al.
    • Organizer
      88th meeting of Kansai Laboratory Animal Research Association
    • Place of Presentation
      Kyoto
    • Year and Date
      2005-12-02
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.vet.osakafu-u.ac.jp/path/path.html

    • Related Report
      2007 Final Research Report Summary
  • [Remarks]

    • URL

      http://www.vet.osakafu-u.ac.jp/path/path.html

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

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