| Project/Area Number |
17580273
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Setsunan University |
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Principal Investigator |
OZAKI Kiyokazu Setsunan University, Faculty of pharmaceutical science, assistant professor (40268496)
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| Co-Investigator(Kenkyū-buntansha) |
NARAMA Isao Setsunan University, Faculty of pharmaceutical science, professor (80268490)
MATSUURA Tetsuro Setsunan University, Faculty of pharmaceutical science, assistant professor (20268494)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | rat / diabetes / peripheral nerve / morphometry / Insulin / peripheral neuropathy |
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| Research Abstract |
Intensive insulin therapy has often brought an increased incidence of hypoglycemia. These patients sometimes developed hypoglycemic neuropathy. However, it still remains unclear that how much of hypoglycemic conditions induce the morphological and clinical changes of peripheral neuropathy. Spontaneously diabetic WBN/Kob rats develop diabetic peripheral motor neuropathy characterized by primary segmental demyelination and secondary axonal degeneration. We, therefore, examined the short-term (30 days) and long-term (50 weeks) effects of hypoglycemia on neuropathic changes in WBN/Kob rats
In short-term study, conduction velocity of insulin-treated normoglycemic/slightly hyperglycemic (60-250 mg/dl) group was higher than that of non-treated spontaneously diabetic (350-420 mg/dl) and hypoglycemic/slightly hyperglycemic (35-200 mg/dl ) groups. Morphological analysis of sciatic-tibial motor nerves of hypoglycemic/slightly hyperglycemic group showed severe changes. These changes tended to occur
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large-sized myelinated fibers. In other groups, only mild changes were seen. The degree and distribution of these degenerated nerve fibers in hypoglycemic/slightly hyperglycemic group were significantly higher than that of other groups. These results suggest that hypoglycemia less than 60 mg/dl by treatment with insulin implants induced severe peripheral neuropathy in diabetic WBN/kob rats.
In long-term study, insulin-treated (60-400 mg/d1) group showed mild morphological changes, but moderate to severe morphological changes were seen in spontaneous diabetic (350-420 mg/dl) group. These results suggest that appropriate blood glucose control inhibit progression of diabetic peripheral motor neuropathy in diabetic WBN/kob rats.
In conclusion, we confirmed hypoglycemia less than 60 mg/dl for short, time induced severe peripheral neuropathy. Therefore, appropriate blood glucose control for diabetic condition is very important and inhibits progression of diabetic peripheral motor neuropathy. In addition, we have proved that WBN/kob rat is a useful animal model for diabetic peripheral neuropathy. Less
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