| Project/Area Number |
17580284
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Clinical veterinary science
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
KOICHI Ushizawa National Institute of Agrobiological Sciences, Biology Research Unit, Researcher (90399428)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Toru National Institute of Agrobiological Sciences, Biology Research Unit, Researcher (20355738)
HASHIZUME Kazuyoshi Iwate University, Faculty of Agriculture, Professor (10355737)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,680,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥180,000)
Fiscal Year 2007: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | bovine / collagen / 7S / uterus / extracellular matrix / immunoassay / 胎盤 / マトリックス / 妊娠 / 免疫測定 / 分娩 / IV型 / 子宮修復 / 抗体 |
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| Research Abstract |
The type IV collagen is the major component of basement membrane and consisted with triplet fibers of collagen molecules. Collagen IV forms supramolecular structure to serve as the scaffold for tissue structure and other basement membrane components. Collagen N comprised three distinct structural domains: 7S domain at the amino terminus; the triple helical domain in the intermediate region; and non-collagenous domain in the carboxyl terminus. Type N collagens associate in a head-to-head fashion to form dimers and in tail-to-tail fashions to form tetramers, to compose sheet-like structure. Among these domains of type N collagen, 7S domains are resistant to collagenases and persistent in the peripheral circulation following matrix remodeling. The concentration of circulating 7S domain could be a good marker for assessment of the tissue remodeling. Bovine uterus dramatically involutes following calving. In the present study, we prepared bovine type N collagen 7S domain and its antiserum for establishing the assessment procedure for uterine involution in the cow. The 7S domain was prepared from bovine placenta. Placental tissues were minced and treated with 8M urea to remove soluble proteins. Residual sediment was collected and used for collagenase digestion. Collagenase digest was chromatographed with Sephacryl S-200HR to isolate high molecular components containing the dodecamer of 7S domain. Isolated fractions were reduced and separated with preparative electrophoresis to collect the monomer of 7S domain. Purified 7S domain was used for antibody generation. In summary we generated bovine type N collagen 7S domain and its antiserum for establishing immunoassay system for measurement of circulating 7S domain concentrations.
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