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Microbial removal of heavy metals from agricultural and marine industrial wastes in a sequential and mixed cultivation

Research Project

Project/Area Number 17580285
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Boundary agriculture
Research InstitutionMuroran Institute of Technology

Principal Investigator

KIKUCHI Shintaro  Muroran Institute of Technology, Department of Applied Chemistry, Professor (70148691)

Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,530,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥30,000)
Fiscal Year 2007: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2005: ¥2,700,000 (Direct Cost: ¥2,700,000)
Keywordsafricultura and marine industrial wastes / microbial removal of heavy metals / sequential and mixed cultivation / heavi metal-binding proteins / proteolytic microbes / sulfate-reducing bacteria / metal-sulfide precipitates / 農水産物廃棄組織 / 硫化水素 / 応用微生物 / 環境技術 / バイオテクノロジー / 廃棄物再資源化 / 微生物 / カドミウム汚染農水産廃棄物 / 硫化カドミウム沈殿
Research Abstract

Microbial removal of heavy metals, especially cadmium, from agricultural and marine industrial wastes were examined. During sequential and mixed cultivation of proteolytic microbes and a strain of sulfate-educing bacteria in an extracts of each waste, heavy metals like cadmium were removed almost completely from the extract as the metal-sulfide precipitate. Whereas a ant amounts of heavy metals remained in the extract after the cultivation of proteolytic microbes or the sulfate-reducing bacterium alone.
These results suggested that heavy metals in agricultural and marine industrial wastes would be present as bound-state with heavy-metal-binding protein such as metallothionein and they would be transformed to the free-state by protease secreted by proteolytic microbes. And then metal-sulfide precipitates could be formed with the free-state of heavy metals and hydrogen sulfide produced by the sulfate-reducing bacterium. Availability of meal-free preparations as. feed were also examined.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (45 results)

All 2008 2007 2006 2005 Other

All Journal Article (21 results) (of which Peer Reviewed: 9 results) Presentation (19 results) Book (3 results) Remarks (2 results)

  • [Journal Article] Completed dechlorination of tetrachloroethylene by use of an anaerobic Clostridium bifermentas DPH-1 and zero-varent iron2008

    • Author(s)
      S. Kikuchi, 他3名
    • Journal Title

      Environmental Technology 29(In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Completed dechlorination of tetrachloroethylene by use of an anaerobic clostridium bifermentans DPH-1 and zero-valent iron2008

    • Author(s)
      Y. C. Choel, S. Kikuchi, N. Kawauchi, K. Takamizawa
    • Journal Title

      Environmental Technology Vol.29(In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 水道管材料が微生物膜形成に及ぼす影響2007

    • Author(s)
      菊池慎太郎, 他4名
    • Journal Title

      環境技術 36

      Pages: 743-746

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 光合成細菌による農産廃棄物からの生理活性物質生産と農業への応用可能性2007

    • Author(s)
      菊池慎太郎, 他5名
    • Journal Title

      水処理技術 48

      Pages: 49-54

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effects of pipe-materials on microbial film formation in drinking water distribution system2007

    • Author(s)
      Young, C. Choel, Tadashi Toyama, Andrew A. Randall, Kazuhiro Takamizawa, Shintaro Kikuchi
    • Journal Title

      Kankyo Gijyutsu Vol.36,No.10

      Pages: 743-746

    • NAID

      130004543473

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Conversion of agricultural waste to bio-active substances by photosynthetic Bacteria (in Japanese)2007

    • Author(s)
      Atsushi, Mizutani, Shigekazu Kitayama, Kazuya Hara, Young C. Choel, Toyama, Yasushi Hasegawa, Shintaro Kikuchi
    • Journal Title

      Mizu Shyori Gijyutsu Vol.48,No.2

      Pages: 49-54

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 光合成細菌による農産廃棄物からの生理活性物質生産と農業への応用可能性2007

    • Author(s)
      水谷敦司(他, 5名)
    • Journal Title

      水処理 48

      Pages: 49-54

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Utility of Measuring Assimilable Organic Carbon as an Indicator Of Bioability in Distribution System for Finished Water2007

    • Author(s)
      Young. C. Chang(他, 2名)
    • Journal Title

      Journal of Environmental Health 32

      Pages: 539-542

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 光合成細菌による農産廃棄物からの生理活性物質生産と農業への応用可能性2007

    • Author(s)
      菊池 慎太郎, 他6名
    • Journal Title

      水処理技術 48巻2号

      Pages: 49-54

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Application of microbes and development of new bio-material for environment conservation2007

    • Author(s)
      Shintaro Kikuchi
    • Journal Title

      2007 International Symposium on Environment-Friendly Technology, Material and Energy

      Pages: 64-76

    • Related Report
      2006 Annual Research Report
  • [Journal Article] レーザー照射による抗菌性酸化チタン材の作成と海洋汚損生物付着防除への応用2006

    • Author(s)
      菊池慎太郎, 他6名
    • Journal Title

      環境技術 35

      Pages: 756-762

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] ビートパルプからのアラビノース抽出と高付加価値化に関する研究2006

    • Author(s)
      菊池慎太郎, 他3名
    • Journal Title

      水処理技術 47

      Pages: 251-256

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The Utility of measuring assimilable organic carbon as an indicator of biostability in distribution system for finished water2006

    • Author(s)
      Shintaro Kikuchi, 他3名
    • Journal Title

      Korean Journal of Environmental Health 32

      Pages: 539-542

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Preparation of antimicrobial TiO2 by laser irradiation and its application to prevent the adhension of marine fouling organisms (in Japanese)2006

    • Author(s)
      Atsushi Mizutani, Masamitsu Shimazu, Tadashi Toyama, Chang Y. Choel, Shintaro Kikuchi
    • Journal Title

      Kankyo Gijyutsu Vol.35,No.10

      Pages: 756-762

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Extraction of arabinose from the agricultural waste, beet-pulp, (in Japanese)2006

    • Author(s)
      Eiji Kobayashi, Tsutom Arizuka, Hrohito Kikuchi, Shintaro Kikuchi
    • Journal Title

      Mizu Shyori Gijyutsu Vol.46,No.6

      Pages: 251-256

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The utility of measuring assimilable organic carbon as an indicator of biostabihty in distribution system for finished water2006

    • Author(s)
      Young C. Chang, Tadashi Toyama, Kweon Jung, Shintaro Kikuchi
    • Journal Title

      Korean Journal of Environmental Health Vol.32,No.6

      Pages: 539-542

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] ビートパルプからのアラビノース抽出と高付加価値化に関する研究2006

    • Author(s)
      菊池 慎太郎, 他3名
    • Journal Title

      水処理技術 46巻・6号

      Pages: 251-256

    • Related Report
      2006 Annual Research Report
  • [Journal Article] レーザー照射による抗菌性酸化チタン材の作成と海洋汚損生物付着防除への応用可能性2006

    • Author(s)
      菊池 慎太郎, 他6名
    • Journal Title

      環境技術 35巻10号

      Pages: 756-762

    • NAID

      10018301703

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The utility of measuring assimiable organic carbon as an indicator of biostability in distribution systems for finished water2006

    • Author(s)
      Y.Chang, S.Kikuchi, 他2名
    • Journal Title

      Korean Journal of Environmental Health Vol.32,No6

      Pages: 539-542

    • Related Report
      2006 Annual Research Report
  • [Journal Article] ホタテガイ貝殻に皮膚角化細胞増殖因子が存在2005

    • Author(s)
      長谷川靖, 菊池慎太郎
    • Journal Title

      化学と生物 43

      Pages: 145-147

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Isolation of keratinocyte growth factor from shells of Scallop (in Japanese)2005

    • Author(s)
      Yasushi Hasegawa and Shintaro Kikuchi
    • Journal Title

      Kagaku to Seibutsu Vo.43,No3

      Pages: 145-147

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Application of Microbes and Development of New Bio-materials for Environmental Conservation2008

    • Author(s)
      Shintaro KIKUCHI
    • Organizer
      International Symposium of Environment-Friendly Technology
    • Place of Presentation
      Seoul National Univ. of Technology, Seoul, Korea
    • Year and Date
      2008-02-13
    • Related Report
      2007 Annual Research Report
  • [Presentation] ヨシ根圏からのプチルフェノール分解菌の分離2007

    • Author(s)
      菊池慎太郎, 他3名
    • Organizer
      日本水処理生物学会2007年年次大会
    • Place of Presentation
      富山県富山市
    • Year and Date
      2007-11-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Isolation of butylphenol-degrading bacteria from rhizospere of Phramites australis (in Japanese)2007

    • Author(s)
      Tadashi Toyama, Tetsuya Okawa, Y. C. Chang, Shintaro Kikuchi
    • Organizer
      Annual Meeting of Japanese Society of Water Treatment Biology 2007
    • Place of Presentation
      Toyama, Japan
    • Year and Date
      2007-11-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Application of microbes and development of new biomaterials for environmental conservation2007

    • Author(s)
      Shintaro Kikuchi
    • Organizer
      International Symposium on environment-friendly technology
    • Place of Presentation
      大韓民国,ソウル市
    • Year and Date
      2007-02-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Application of microbes and development of new environmental conservation2007

    • Author(s)
      Shintaro Kikuchi
    • Organizer
      International Symposium on biomaterials for environmental-friendly technology
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2007-02-27
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Preparation of antimicrobial TiO2 by laser irradiation and its Application to prevent the formation of biofilm2007

    • Author(s)
      Shintaro Kikuchi , et. al.
    • Organizer
      The 10th Seoul-Osaka Synposium on Public Health and Environmental Issues
    • Place of Presentation
      大韓民国,ソウル市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 低温環境下重質油汚染地域から分離した重質油分解菌の性質2007

    • Author(s)
      菊池慎太郎, 他3名
    • Organizer
      弟59会日本生物工学会年次大会
    • Place of Presentation
      広島県東広島市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ヨシ根圏からのピレン分解菌の分離と特徴2007

    • Author(s)
      菊池慎太郎, 他6名
    • Organizer
      弟59会日本生物工学会年次大会
    • Place of Presentation
      広島県東広島市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 高炉ダストを再活用した環境汚染物質の新規処理技術の開発2007

    • Author(s)
      菊池慎太郎, 他3名
    • Organizer
      弟44回環境工学フォーラム
    • Place of Presentation
      山口県宇部市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Preparation of antimicrobial TiO2 by laser irradiation and its application to prevent the formation of biofim2007

    • Author(s)
      Shintaro Kikuchi, Young-Choel Chang, Tadashi Toyama
    • Organizer
      The 10th Seoul Osaka Symposium on of Public Health and Environmental Issues
    • Place of Presentation
      Seoul, Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Isolation and characterization of lubricant oil-degrading bacteria from waste lubricant-contaminated soil under low temperature conditions (in Japanese)2007

    • Author(s)
      Young C. Chang, Masakuni Sasaki, Tadashi Toyama, Shintaro Kikuchi
    • Organizer
      The 59th Annual Meeting of Japan Society of Bioengineering and Biotechnology
    • Place of Presentation
      Hiroshima, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Isolation of ptren-degrading bacteria from rhizosphere of Phragmites australis (in Japanese)2007

    • Author(s)
      Tadashi Toyama, Tetsuya Furukawa, Sei Kazunari, Kun Soda, Y. C. Chang Shintaro Kikuchi Michiko Ike
    • Organizer
      The 59th Annual Meeting of Japan Society of Bioengineering and Biotechnology
    • Place of Presentation
      Hiroshima, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of a new environmental type processing technology using waste blast furnance dust (in Japanese)2007

    • Author(s)
      Young. C. Chang, Masakni Sasaki, Tadashi Toyama, Shintaro Kikuchi
    • Organizer
      The 44th Annual Meeting of Environmental Engineering, Japan Society of Civil Engineers
    • Place of Presentation
      Ube, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] テトラクロロエチレン分解用バイオリアクターの特性2006

    • Author(s)
      菊池慎太郎, 他6名
    • Organizer
      日本化学工学会2006年度大会
    • Place of Presentation
      北海道室蘭市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ウキクサ根圏における芳香族化合物の効率的分解2006

    • Author(s)
      菊池慎太郎, 他6名
    • Organizer
      日本化学工学会2006年度大会
    • Place of Presentation
      北海道室蘭市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 上水管路に付着した生物膜のDNAプローブによる直接定量2006

    • Author(s)
      菊池慎太郎, 他4名
    • Organizer
      日本化学工学会2006年度大会
    • Place of Presentation
      北海道室蘭市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Characterization of a tetraxhloro-ethylene degrading bioreactor (in Japanese)2006

    • Author(s)
      Young C. Chang, Tadashi Toyama, Shintaro Kikuchi, Takeshi Sano, Junko Kawauchi, Kazuhiro Takamizawa
    • Organizer
      2006 Annual Metting of Japanese Chemical Engineerong Society
    • Place of Presentation
      Muroran, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effective degration of aromatic compounds in rizosphere of Phrabmites australis (in Japanese)2006

    • Author(s)
      Tadashi Toyama, Y. C. Chang, Shintaro, Kikuchi, Sei Kazunari, Machihiko Ike, Masanori Fujita
    • Organizer
      2006 Annual Metting of Japanese Chemical Engineerong Society
    • Place of Presentation
      Muroran, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Direct estimation of biofilm density on pipe in drinking water distribution system using a DNA-probe (in Japanese)2006

    • Author(s)
      Young C. Chang, Tadashi Toyama, Shintaro Kikuchi, A. A. Randall
    • Organizer
      2006 Annual Metting of Japanese Chemical Engineerong Society
    • Place of Presentation
      Muroran, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] バイオフィルムの基礎と応用(「第二章表面改質によるバイオフィルム形成防止技術」を分担)2008

    • Author(s)
      菊池慎太郎(分担執筆)
    • Total Pages
      399
    • Publisher
      株式会社エヌティエス出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] バイオフィルムの基礎と制御2007

    • Author(s)
      菊池 慎太郎
    • Total Pages
      399
    • Publisher
      株式会社エヌ・ティ・エス
    • Related Report
      2007 Annual Research Report
  • [Book] 微生物工学 弟2版2006

    • Author(s)
      菊池 慎太郎, 他4名
    • Total Pages
      169
    • Publisher
      三共出版株式会社
    • Related Report
      2006 Annual Research Report
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.muroran-it.ac.jp/

    • Related Report
      2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.mmm.muroran-it.ac.jp/~tadashi/

    • Related Report
      2007 Final Research Report Summary

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

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