Investigations of potential use of dark septate endophytes
| Project/Area Number |
17580288
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Boundary agriculture
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| Research Institution | Shinshu University |
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Principal Investigator |
KUGA Yukari Shinshu University, Faculty of Agriculture, Associate Professor (30232747)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,710,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Dark sentate endonhvte / Field grown lettuce / Arbuscular mvcorrhiza / Lettuce root rot / Phylozeny / Biological control / Induced systemic resistance / Colonization process / 植物根部内生糸状菌 / 誘導抵抗性 / アーバスキュラー菌根菌 / ポリリン酸 / 根部エンドファイト / 菌類 / 菌根 / Dark septate endophyte |
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| Research Abstract |
Fungal root endophytes were studied with regard to their potential use as biological control agents against lettuce mot rot (pathogen: F. oxysporum f. sp. Iactucae; Fol). Studies were initiated using field-grown lettuce in Kawakami village, Nagano prefecture; where colonization rates of dark septate endophytes (DSE) were often much lower than those of arbuscular mycorrhizal fungi (AMF). Colonization frequencies of DSEs, however, were higher than those of AMF. Although AMF colonization rate was negatively correlated with the amount of available phosphorus, none of the soil chemical properties studied correlated to the colonization rate of DSEs. One dominant DSE morphotype (Type-a), was found to exist consistently in 2 experimental years examined. Phylogenetic analysis using rDNA sequence data showed type-a fungi form a sister clade with fungi in the Order Lulworthiales (Sorclariomycetes). Closely related DSE morphotypes were also isolated from Japanese pear tree roots and mountain soil baited with lettuce seedlings. A root box inoculation method showed a high affinity of type-a to lettuce roots: colonization of type-a in lettuce roots occurred 1 day after inoculation(dai), intracellular microsclemtia formation occurred 3 dai, and 100% colonization rate occurred 5 dai Papilla were formed in lettuce root tissues inoculated with either Fol, or non-pathogenic F oxysporum isolated from a lettuce root, a phenomenon which was not observed by type-a colonization. Biological control effects were different among type-α isolates used. Gene expression related to induced systemic resistance (ISR:MAPK, PDF and VSP) and systemic acquired resistance (SAR: PR1a) in lettuce plants colonized with type-a in the roots and challenge inoculated with Erwinia soft-rot bacteria on the leaves were analyzed by quantitative RTPCR. The results indicated that type-a may stimulate ISR but not SAR.
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Report
(4 results)
Research Products
(73 results)
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[Journal Article] Ultrastructure of rapidly-frozen and freeze-substituted germ tubes of an arbuscular mycorrhizal fungus, and localization of polyphosphate2008
Author(s)
Kuga, Y., Saito, K., Nayuki, K., Peterson, R. L., Saito, M
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Journal Title
New Phytologist 178(1)
Pages: 189-200
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] septate endophytes : isolation, characterization and potential use as biological control agents against soil-borne disease. (in Jananese)2006
Author(s)
Kamiya, T., Taniguchi, M., Bourgault, R., Yoshida, S., Kuga, Y. Dark
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Journal Title
Annual Report of Interdisciplinary Research Institute of Environmental Sciences 25
Pages: 57-72
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] labeling of polyphosphate at the ultrastructural level in Saccharomyces cerevisiae using affinity of the polyphosphate binding domain of Escherichia coli exopolyphosphatase2005
Author(s)
Saito, K., Ohtomo, R., Kuga-Uetake, Y., Aono, T., Saito, M., Direct
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Journal Title
Applied and Environmental Microbiology 71
Pages: 5692-5701
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Dark septate endophytes isolated from the roots of lettuce and Japanese pear trees include novel members closely aligned to the Lulworthiales.2007
Author(s)
Bourgault, R., Nakamura, K., Kamiya, T., Arai, T., Yaguchi, H., Kuga, Y.
Organizer
Asian Mycology Congress and Xth International Marine and Freshwater Mycology Symposium.
Place of Presentation
Penang, Malaysia
Description
「研究成果報告書概要(和文)」より
Related Report
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[Presentation] Ectomycorrhiza dynamics in Japanese red pine forests observed by a minirhizotron system2007
Author(s)
Miyamoto, Y., Masuno, K., Kobayashi, H., Ogura, T., Yamada, A., Kuga, Y
Organizer
The 51st Annual Meeting of the Mycological Society of Japan
Place of Presentation
Tsukuba
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Root endophytes in Japanese pear trees2007
Author(s)
Bourgault, R., Arai, T., Nakamura, H., Eguchi, N., Kuga, Y
Organizer
The 23rd Annual Meeting of the Japanese Society of Microbial Ecology, September 15th-18th, 2007.
Place of Presentation
Matsuyama
Description
「研究成果報告書概要(欧文)」より
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[Presentation] ATP dependent polyphosphate synthesis in arbuscular mycorrhizal fungi : induction by phosphate and enzymatic characteristics2007
Author(s)
Tani, C., Nayuki, K., Murase, M., Kuga, Y., Ohtomo, R., Oosaki, M., Ezawa, T
Organizer
The 17th Annual Meeting of Japanese Society of Plant Microbe Interactions
Place of Presentation
Kagoshima
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Dark septate endophytes isolated from the roots of lettuce and Japanese pear trees include novel members closely aligned to the Lulworthiales2007
Author(s)
Bourgault, K., Nakamura, K., Kamiya, T., Arai, T., Yaguchi, H., Kuga, Y
Organizer
Asian Mycology Congress and Xth International Marine and Freshwater Mycology Symposium
Place of Presentation
Penang, Malaysia
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Dispersed and aggregated forms of polyphosphate are observed in freeze-substituted intraradical hyphae and germ tubes of Gigaspora margarita2006
Author(s)
Kuga, Y., Nayuki, K., Saito, K., Peterson, R.L., Saito, M
Organizer
Fifth International Congress on Mycorrhizas
Place of Presentation
Granada, Spain
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Ultrastructure of arbuscular mycorrhizal fungus and localization of polyphosphate2006
Author(s)
Saito, K., Kuga, Y., Naito, Y., Ohtomo, R., Hamaguchi, H., Saito, M
Organizer
The 16th Annual Meeting of Japanese Society of Plant Microbe Interactions
Place of Presentation
Sapporo
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Ectomycorrhiza dynamics in Japanese red pine forests observed by a minirhizotron system2006
Author(s)
Miyamoto, Y., Masuno, K., Kobayashi, H., Ogura, T., Yamada, A., Kuga, Y
Organizer
Japanese Conference on Mycorrhizas 2006, November 3rd-4th, 2006.
Place of Presentation
Minami-minowa
Description
「研究成果報告書概要(欧文)」より
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