Development of glycoddrug using antigenic oligosaccharides that regulate the production of cytokins
Project/Area Number |
17580300
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied molecular and cellular biology
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Research Institution | Okayama University |
Principal Investigator |
KIMURA Yoshinobu Okayama University, Dept. of Biofunctional Chemistry, Graduate School of Natural Science and Technology, Professor (70195387)
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Project Period (FY) |
2005 – 2007
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Project Status |
Completed (Fiscal Year 2007)
|
Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
|
Keywords | pollen allergen / plant glycoprotein / antigenic oligosaccharide / plant N-glycan / tumor marker oligosaccharide / 抗原性糖ペプチド / イチョウ種子糖蛋白質 / 腫瘍抗原 / T-抗原 / ローヤルゼリータンパク質 / ポリグルタミン-糖複合体 / 植物糖蛋白質 / Lewis aエピトープ / ヒノキ花粉アレルゲン / スギ花粉アレルゲン |
Research Abstract |
The chemical structures of strong antigenic N-glycans perpared from three major pollen allergens, Cry j 1 (Japanese cedar pollen allergen), Jun a 1 (Mountain cedar pollen allergen), and Cha o 1 (Japanese cypress pollen allergen). In the case of Cry j 1 and Jun a 1, we found that Lewis a epitope (Galβ1-3 (Fucα1-4) GlcNAc) occurs in the N-glycan moieties, in addition to the GlcNAc2Man3Xyl1Fuc1GlcNAc2 structure. We analyzed three-dimensional pattern of the antigenic N-glycans occurring ubiquitously on pollen allergens using computer modeling and we found a difference between the 3D pattern of free antigenic N-glycans and that of N-glycans linked to polypeptide-back bone. We also developed a conjugation method of the antigenic glycopeptides and synthetic polymer (polyglutamic acid) to investigate immunogenic activity of antigenic N-glycans against B-cells and T-cells. We also found a new tumor antigen oligosaccharide, T-antigen (Galβ1-3Ga1NAc), in royal jelly glycoproteins, which can be used as an anti-tumor glycodrug for antibody production.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Glycoform analysis of Japanese cypress pollen allergen, Cha o 1: Comparison of glycoform of cedar and cypress pollen allergens2008
Author(s)
Kimura, Y., Kuroki, M., Maeda, M., Okano, M., Yokoyama, M., Kino, K
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Journal Title
Biosci. Biotechnol. Biochem 72
Pages: 514-522
NAID
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] Glycoform analysis of Japanese cypress pollen allergen, Cha o 1 : Comparison of glycoform of cedar and cypress pollen allergens2008
Author(s)
Kimura, Y., Kuroki, M., Maeda, M., Okano, M., Yokoyama, M., Kino, K
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Journal Title
Biosci. Biotechnol. Biochem 72
Pages: 485-491
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Glycoform analysis of Japanese cypress pollen allergen, Cha o l: Comparison of glycoform of cedar and cypress pollen allergens2008
Author(s)
Kimura, Y., Kuroki, M., Maeda, M., Okano, M., Yokoyama, M., and Kino, K
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Journal Title
Biosci. Biotechnol. Biochem 72
Pages: 485-491
Related Report
Peer Reviewed
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[Journal Article] Salt-adaptation of Tobacco BY2 cells induces change in glycoform of N-glycans.2008
Author(s)
Kimura, Y., Watanabe, T., Kimura, M., Maeada, M., Murata, Y., Fujiyama, K
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Journal Title
Biosci. Biotechnol. Biochem 72
Pages: 514-522
Related Report
Peer Reviewed
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[Journal Article] Evidence for new β1-3 galactosyltransferase activity involved in biosynthesis of unusual N-glycan harboring T-antigen in Apis mellifera.2007
Author(s)
Kimura, Y., Sakamura, S., Ushijima, T., Hama, Y., Kajiura, H., Fujiyama, K., Okihara, K., Hashimoto, K.,
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Journal Title
Biosci. Biotechnol. Biochem 71
Pages: 1111-1114
NAID
Related Report
Peer Reviewed
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[Journal Article] Glycoform analysis of Japanese cedar pollen allergen, Cry j 1.2005
Author(s)
Maeda, M., Kamamoto, M., Hino, K., Yamamoto, S., and Kimura, Y
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Journal Title
Biosci. Biotechnol. Biochem 69
Pages: 1700-17005
NAID
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] Occurrence of Lewis a epitope in N-glycans of a glycoallergen, Jun a 1, from mountain cedar (Juniperus ashei) pollen.2005
Author(s)
Kimura, Y., Kamamoto, M., Maeda, M., Okano, M., Yokoyama, and M., Kino, K.
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Journal Title
Biosci. Biotechnol. Biochem 69
Pages: 137-144
NAID
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] Glycoform analysis of Japanese cedar pollen allergen, Cry j 12005
Author(s)
Maeda, M., Kamamoto, M., Hino, K., Yamamoto, S., Kimura, Y
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Journal Title
Biosci. Biotechnol. Biochem 69
Pages: 1700-17005
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Occurrence of Lewis a epitope in N-glycans of a glycoallergen, Jun a 1, from the mountain cedar pollen2005
Author(s)
Kimura, Y., Kamamoto, M., Maeda, M., Okano, M., Yokoyama, M., Kino, K
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Journal Title
Biosci. Biotechnol. Biochem 69
Pages: 137-144
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Identification of royal jelly glycoprotein bearing N-glycans with T-antigen unit2007
Author(s)
Nagai, H., Kimura, M., Okihara, K., Hashimoto, K., Sugimoto, H., Yamada, H., Kimura, Y.
Organizer
XIX th International Symposium on Glycoconjugates
Place of Presentation
ケアンズ・オーストラリア
Year and Date
2007-07-17
Related Report
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