| Project/Area Number |
17580300
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Okayama University |
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Principal Investigator |
KIMURA Yoshinobu Okayama University, Dept. of Biofunctional Chemistry, Graduate School of Natural Science and Technology, Professor (70195387)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | pollen allergen / plant glycoprotein / antigenic oligosaccharide / plant N-glycan / tumor marker oligosaccharide / 抗原性糖ペプチド / イチョウ種子糖蛋白質 / 腫瘍抗原 / T-抗原 / ローヤルゼリータンパク質 / ポリグルタミン-糖複合体 / 植物糖蛋白質 / Lewis aエピトープ / ヒノキ花粉アレルゲン / スギ花粉アレルゲン |
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| Research Abstract |
The chemical structures of strong antigenic N-glycans perpared from three major pollen allergens, Cry j 1 (Japanese cedar pollen allergen), Jun a 1 (Mountain cedar pollen allergen), and Cha o 1 (Japanese cypress pollen allergen). In the case of Cry j 1 and Jun a 1, we found that Lewis a epitope (Galβ1-3 (Fucα1-4) GlcNAc) occurs in the N-glycan moieties, in addition to the GlcNAc2Man3Xyl1Fuc1GlcNAc2 structure. We analyzed three-dimensional pattern of the antigenic N-glycans occurring ubiquitously on pollen allergens using computer modeling and we found a difference between the 3D pattern of free antigenic N-glycans and that of N-glycans linked to polypeptide-back bone.
We also developed a conjugation method of the antigenic glycopeptides and synthetic polymer (polyglutamic acid) to investigate immunogenic activity of antigenic N-glycans against B-cells and T-cells.
We also found a new tumor antigen oligosaccharide, T-antigen (Galβ1-3Ga1NAc), in royal jelly glycoproteins, which can be used as an anti-tumor glycodrug for antibody production.
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