| Project/Area Number |
17590033
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | The University of Tokushima |
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Principal Investigator |
TANAKA Hideji The University of Tokushima, Institute of Health Biosciences, Professor, 大学院ヘルスバイオサイエンス研究部, 教授 (40207121)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | flow titration / flow ratiometry / flow analysis / feedback-based control / titrimetry / high throughput titration |
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| Research Abstract |
Flow ratiometry, which had been developed by the head investigator in 2000, was applied to titrimetry with high throughput rate. In the developed system, titrant flow rate is linearly increased/decreased in accordance with control signal from a computer, while total (titrant + titrand) flow rate was hold constant. A program was newly written in house for full automation of controlling the system, acquiring analytical signal and analyzing the data. The algorithm was constituted of two operation modes. Feedback-based variable triangular wave control mode was used for locating the equivalence point. Scan direction of the control signal was changed from upward to downward, and vice versa, at an instant of the detection of the equivalence point. Subsequent fixed triangular wave control mode was used for further limiting the scan range just around the equivalence point. Thus, the scan in needless range can be avoided and unprecedented high throughput rate (maximally 34 titrations per minute) was realized. The method was successfully applied to acid-based and chelatometric titrations with photometric or potentlometric detection. However, simultaneous titration of calcium and magnesium ions using both photometric and potentiometric sensors still has a problem to be solved : precision of the analytical results is not so high. The method was also applied to the automatic and rapid determination of the electrolytic dissociation constants of acids and bases at various conditions.
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