OXIDATIVE STRESS BY SE-DEFICIENCY AND DYNAMICS OF BIOTRACE ELEMENTS

• Project/Area Number: 17590040
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Showa Pharmaceutical University
• Principal Investigator: ENDO Kazutoyo Showa Pharmaceutical University, DEPT. OF PHARMACY, PROFESSOR (50106598)
• Co-Investigator(Kenkyū-buntansha): HONDA Chikako SHOWA PHARMACUETICAL UNIVERSITY, DEPT. OF PHARMACY, Associate Professor (00199572) ; MATSUOKA Keisuke SHOWA PHARMACUETICAL UNIVERSITY, DEPT. OF PHARMACY, RESEARCH ASSOCIATE (90384635) ; ENOMOTO Shuichi RIKEN, 加速器基盤研究部, REASERCH FELLOW (10271553)
• Project Period (FY): 2005 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥3,680,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥180,000); Fiscal Year 2007: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2006: ¥600,000 (Direct Cost: ¥600,000); Fiscal Year 2005: ¥2,300,000 (Direct Cost: ¥2,300,000)
• Keywords: TRECE EMEMENT / SE-DEFICIENCY / OXIDATIVE STRESS / ESR / GSH-Px / RATS / ROS / 生体微量元素 / Se-欠乏ラット / 肝ミクロソーム / ESR-スピンプローブ / X-バンドESR / 非侵襲測定 / 低線量β線照射 / Se-欠乏 / ニトロキシスピンプローブ

Research Abstract

1) In vivo free radical reactions in rat liver as a result of exposure to low-dose β-radiation was evaluated with electron paramagnetic resonance (EPR) spectroscopy by monitoring the reduction of the nitroxyl spin probe after intravenous administration. Results suggest that under low levels of tissue oxygen, exposure to β-rays results in nitroxyl signal loss, which may be mediated by free radical dependent pathways.
2) Biochemical data of AST, ALT, and BUN for the plasma fraction of the rat bloods, and the concentration of vitamin C and vitamin E were measured in the liver homogenates. The relationships among the biotrace elements and the biochemical data were compared, and the indices for oxidative stress caused by selenium deficiency were discussed in terms of reactive oxygen species (ROS) , and antioxidants in the organism.
3) Free radical-reducing activity and membrane fluidity of liver microsomes from Se-deficient (SeD) Wistar rats were examined by EPR spin label method using nitrox yl-labeled stearic acids. SeD induced the liver microsomal cytochrome P 450 activity ; the nitroxyl spin probes are substrates for reduction-relating cytochrome P 450. The reduction rate of nitroxyl radical at shallow depth in membranes was increased.
4) Although the oxidative stress caused Fe accumulation in the liver, the Fe concentration in bile of the SeD rat was almost the same as that in the control rats. Due to the trapping of reactive oxygen species, only bilirubin concentrations in the bile and plasma were decreased in the SeD group, when compared with those in the control group. Measurement of bilirubin concentration may be used as a supplemental oxidative stress marker.
5) Dynamics of redox relating biotrace elements, selenium, iron, and zinc in liver, kidney, and spleen of selenium deficient Wistar male rats in a series of feeding period (from 0 to 8 weeks) were studied using instrumental neutron activation analysis. The results were compared with those of aspartate aminotransferase (AST) , alanine aminotransferase (ALT) , and blood urea nitrogen (BUN) for the plasma fraction of the rat bloods.

Research Products

• [Journal Article] Changes in the components of biliary and plasma lipids in selenium-deficient rats (2007)
  • Author(s): Y.Sakuma, J.Sasaki, A.Futami, K.Yamasaki, K.Matsuoka, C.Honda, K.Endo and M.Tsukada
  • Journal Title: Chemistry and Physics of Lipid 148 Pages: 70-76
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Changes in the components of biliary and plasma lipids in selenium-deficient rats (2007)
  • Author(s): Yasunobu, Sakuma, Junya Sasaki, Aya, Futami, Kousuke, Yamasaki, Keisuke, Matsuoka, Chikako, Honda, Kazutoyo, Endo, Masamichi, Tsukada
  • Journal Title: Chem. Phys. Lipids 148(2) Pages: 70-76
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Changes in thecomponents of biliary and plasma lipidicient rats (2007)
  • Author(s): Y.Sakuma, J.Sasaki, A.Futami, K.Yamasaki, K.Matsuoka, C.Honda, K.Endo and M.Tsukada
  • Journal Title: Chemistry and Physics of Lipids 148 Pages: 70-76
  • Peer Reviewed
• [Journal Article] Feeding period of selenium-deficient diet and response of redox relating minerals (2006)
  • Author(s): Matsumoto, Ken-ichiro;Ariyoshi, Marie;Terada, Sachiyo;Okajo, Aya;Urata, Hiromi;Sakuma, Yasumobu;Satoh, Kazue;Ushio, Fusao;Tsukada, Masamichi;Endo, Kazutoyo
  • Journal Title: J.Health Scie 52 Pages: 694-702
  • NAID: 110004863674
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Electron paramagnetic resonance decay constant and oxidative stresses in liver microsomes of the selenium-deficient rat (2006)
  • Author(s): Matsumoto, Ken-ichiro;Suzuki, Ayako;Washimi, Haruka;Hisamatsu, Atsushi;Okajo, Aya;Ui, Iori;Endo, Kazutoyo
  • Journal Title: J.Nutr.Biochem 17 Pages: 677-681
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Feeding period of selenium-deficient diet and response of redox relating minerals (2006)
  • Author(s): Matsumoto, Ken-ichiro, Ariyoshi, Marie, Terada, Sachiyo, Okajo, Aya, Urata, Hiromi, Salcuma, Yasunobu, Satoh, Kazue, Ushio, Fusao, Tsukada, Masamichi, Endo, Kazutoyo
  • Journal Title: J. Health Sci 52(6) Pages: 694-702
  • NAID: 110004863674
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Electron paramagnetic resonance decay constant and oxidative stresses in liver microsomes of the selenium-deficient rat (2006)
  • Author(s): Matsumoto, Ken-ichiro, Suzuki, Ayako, Washimi, Haruka, Hisamatsu, Atsushi, Okajo, Aya, Ui, Iori, Endo, Kazutoyo
  • Journal Title: J.Nutr. Biochem 17(10) Pages: 677-681
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Feeding period of selenium-deficient diet and response of redox relating minerals (2006)
  • Author(s): Matsumoto, Ken-ichiro, Ariyoshi, Marie, Terada, Sachiyo, Okajo, Aya, Urata, Hiromi, Sakuma Yasunobu, Satoh Kazue, Ushio, Fusao, Tsukada, Masamichi, Endo, Kazutoyo
  • Journal Title: J. Health Scie. 52(6) Pages: 694-702
  • NAID: 110004863674
• [Journal Article] Electron paramagnetic resonance decay constant and oxidative stresses in liver microsomes of the selenium-deficient rat (2006)
  • Author(s): Matsumoto, Ken-ichiro, Suzuki, Ayako, Washimi, Haruka, Hisamatsu, Atsushi, Okajo, Aya, Ui, Iori, Endo, Kazutoyo
  • Journal Title: J. Nutr. Biochem 17(10) Pages: 677-681
• [Journal Article] Competition of nitroxyl contrast agents as an in vivo tissue redox probe : Comparison of pharmacokinetics by the bile flow monitoring (BFM) and blood circulating monitoring (BCM) methods using X-band EPR and simulation of decay profiles. (2006)
  • Author(s): Okajo, Aya, Matsumoto, Ken-ichiro, Mitchell, James B., Krishna, Murali C., Endo. Kazutoyo
  • Journal Title: Mag. Res. Med. 56(2) Pages: 422-431
• [Journal Article] Importance of volume limitation for tissue redox status measurements using nitroxyl contrast agents : A comparison of X-band EPR bile flow monitoring (BFM) method and 300MHz in vivo EPR measurement (2006)
  • Author(s): Ui, Iori, Okajo, Aya, Endo, Kazutoyo, Utsumi, Hideo, Matsumoto, Ken-ichiro
  • Journal Title: J. Mag. Res. 181(1) Pages: 107-112
• [Journal Article] Estimation of free radical formation by β-ray irradiation in rat liver (2005)
  • Author(s): Matsumoto, Ken-ichiro;Okajo, Aya;Kobayashi, Takenori;Mitchell, James B.;Krishna, Murali C.;Endo, Kazutoyo.
  • Journal Title: J.Biochem.Biophys.Meth. 63 Pages: 79-90
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Correlation between Ketone body level in selenium-deficient rats and oxidative damages (2005)
  • Author(s): Matsumoto, Ken-ichiro;Takuwa, Aki;Terashi, Akemi;Ui, Iori;Okajo, Aya;Endo, Kazutoyo.
  • Journal Title: Biolo.Pharm.Bull 28 Pages: 1142-1147
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] In vivo multitracer analysis technique:Screening of radioactive probes for noninv asive measurement of physiological functions in experimental animals (2005)
  • Author(s): Matsumoto, Ken-ichiro;Hirunuma, Rieko;Enomoto, Shuichi;Endo, Kazutoyo.
  • Journal Title: Biol.Pharm.Bull 28 Pages: 2029-2034
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Estimation of free radical formation by β-ray irradiation in rat liver (2005)
  • Author(s): Matsumoto, Ken-ichiro, Okajo, Aya, Kobayashi, Takenori, Mitchell, James B., Krishna, Murali, C., Endo, kazutoyo
  • Journal Title: J.Biochem.Biophys.Meth 63(2) Pages: 79-90
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Correlation between Ketone body level in selenium-deficient rats and oxidative damages (2005)
  • Author(s): Matsumoto, Ken-ichiro, Takuwa, Aid, Takenori, Akemi, Ui, Iori, Okajo, Aya, Endo, Kazutoyo
  • Journal Title: Biol.Pharm.Bull 28(7) Pages: 1142-1147
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] In Vivo Multitracer Analysis Technique : Screening of Radioactive Probes for Noninvasive Measurement of Physiological Functions in Experimental Animals (2005)
  • Author(s): K., Matsumoto, R., Hirunuma, S., Enomoto, K., Endo
  • Journal Title: Biol. Pharm.Bull 28(11) Pages: 2029-2034
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] In vivo multitracer analysis technique : Screening of radioactive probes for noninvasive measurement of physiological functions in experimental animals (2005)
  • Author(s): Matsumoto, Ken-ichiro, Hirunuma, Rieko, Enomoto, Shuichi, Endo, Kazutoyo.
  • Journal Title: Biol. Pharm. Bull. 28(11) Pages: 2029-2034
• [Journal Article] Correlation between Ketone body level in selenium-deficient rats and oxidative damages. (2005)
  • Author(s): Matsumoto, Ken-ichiro, Takuwa, Aki, Terashi, Akemi, Ui, Iori, Okajo, Aya, Endo, Kazutoyo.
  • Journal Title: Biolo. Pharm. Bull. 28(7) Pages: 1142-1147
• [Journal Article] Estimation of free radical formation by β-ray irradiation in rat liver. (2005)
  • Author(s): Matsumoto, Ken-ichiro, Okajo, Aya, Kobayashi, Takenori, Mitchell, James B., Krishna, Murali C., Endo, Kazutoyo.
  • Journal Title: J. Biochem. Biophys. Meth. 63(2) Pages: 79-90
• [Presentation] Oxidative stress of selenium deficiency and mineral balance in rats (2008)
  • Author(s): Y.Sakuma, K.Matsuoka, C.Honda, K.Matsumoto, and K.Endo
  • Organizer: Int.Symp.Metallomics2007(ISM 2007)
  • Place of Presentation: Nagoya
• [Presentation] Dynamics of redox related elements and oxidative stress caused by Se-deficiency in rats (2007)
  • Author(s): Y.Sakuma, K.Matsuoka, C.Honda, K.Matsumoto, K.Endo
  • Organizer: Modern trend of activation analysis (MTAA-12)
  • Place of Presentation: Tokyo
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Oxidative stress of selenium deficiency and mineral balance in rats (2007)
  • Author(s): Y.Sakuma, K.Matsuoka, C.Honda, K.Matsumoto, and K.Endo
  • Organizer: Int.Symp.Metallomics 2007 (ISM 2007)
  • Place of Presentation: Nagoya
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Dynamics of redox related elements(Fe, Co, Zn and Se) and oxidative stress caused by Se-deficiency in rats (2007)
  • Author(s): Y., Sakuma, K., Matsuoka, C., Honda, K., Matsumoto, K., Endo
  • Organizer: 12 th Int. Conf.'Moden trends activation analysis(MTAA-12) Hachiohji
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Oxidative stress of selenium deficiency and mineral balance in rats (2007)
  • Author(s): Yasunobu, Sakuma, Keisuke, Matsuoka, Chikako, Honda, Ken-ichiro, Matsumoto, Kazutoyo, Endo
  • Organizer: Int. Symp.Metallomics 2007(ISM 2007) Nagoya
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Dynamics of redox related elements and oxidative stress caused by Se-deficiency in rats (2007)
  • Author(s): Y.Sakuma, K.Matsuoka, C.Honda, K.Matsumoto, K.Endo
  • Organizer: Modern trend of activation analysis(MTAA-12)
  • Place of Presentation: Tokyo
• [Presentation] Se-欠乏による酸化ストレスと肝細胞画分の微量元素 (2007)
  • Author(s): 佐久間 泰亘、長山 敦子、松岡 圭介、本田 智香子、松本 謙一郎、遠藤 和豊
  • Organizer: 日本放射化学会年会
  • Place of Presentation: 静岡大学