Preparation of Nano-, Microparticles for Introduction of Therapeutic Protein and Anti-tuberculosis Drug into Cells

• Project/Area Number: 17590041
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: OZEKI Tetsuya Tokyo University of Pharmacy and Life Science, 薬学部, 准教授 (60277259)
• Co-Investigator(Kenkyū-buntansha): OKADA Hiroaki 東京薬科大学, 薬学部, 教授 (10339096)
• Project Period (FY): 2005 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥2,500,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2007: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000); Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000)
• Keywords: 薬学 / 粒子設計 / DDS / 感染症

Research Abstract

遺伝子組換えタンパク質の活性ペプチドであるp53pと細胞膜を貫通する作用を有するAntennapedia(Ant)を融合させたp53p-Antをマイクロカプセルに封入し、ラット脳腫瘍モデルの腫瘍内で長期にわたり放出させることで生存日数が延長し、抗腫瘍効果が得られた。抗結核薬のリファンピシン(RFP)ナノ粒子の経肺投与製剤を調製し、結核菌が潜む肺胞マクロファージ内に高効率で取り込ませることに成功した。

Research Products

• [Journal Article] One-step preparation of rifampicin/poly(lactic-co-glycolic acid) nano-article-containing mannitol microspheres using a four-fluid nozzle spray drier for inhalation therapy of tuberculosis (2009)
  • Author(s): Katsuya Ohashi, Takahiro Kabasawa, Tetsuya Ozeki, and Hiroaki Okada
  • Journal Title: J. Control. Release 135 Pages: 19-24
  • Peer Reviewed
• [Journal Article] One-step preparation of rifampicin/poly (lactic-co-glycolie acid) nano-article-containing mannitol microspheres using a four-fluid nozzle spray drier for inhalation therapy of tuberculosis (2009)
  • Author(s): Katsuya Ohashi, Takahiro Kabasawa, Tetsuya Ozeki, Hiroaki Okada
  • Journal Title: J. Control. Release 135 Pages: 19-24
  • Peer Reviewed
• [Journal Article] Application of a four-fluid nozzle spray drier to prepare inhalable rifampicin-containing mannitol microparticles (2008)
  • Author(s): Takuto Mizoe, Tetsuya Ozeki, and Hiroaki Okada
  • Journal Title: AAPS PharmSciTech 9 Pages: 755-761
  • Peer Reviewed
• [Journal Article] Application of a four-fluid nozzle spray drier to prepare inhalable rifampicin-containing mannitol microparticles (2008)
  • Author(s): Takuto Mizoe, Tetsuya Ozeki, Hiroaki Okada
  • Journal Title: AAPS PharmSciTech 9 Pages: 755-761
  • Peer Reviewed
• [Journal Article] ナノ粒子含有マイクロスフェアの設計と経肺投与製剤への応用 (2006)
  • Author(s): 尾関哲也、岡田弘晃
  • Journal Title: 薬剤学 66 Pages: 249-253
  • NAID: 10018058438
• [Journal Article] ナノ薬物粒子含有マイクロスフェアによる経肺投与DDS (2006)
  • Author(s): 尾関哲也、岡田弘晃
  • Journal Title: 日本エアロゾル研究 21 Pages: 10-15
  • NAID: 10017973650
  • Peer Reviewed
• [Journal Article] ナノ薬物粒子含有マイクロスフェアによる経肺投与DDS (2006)
  • Author(s): 尾関 哲也
  • Journal Title: 日本エアロゾル研究 21・1 Pages: 10-15
  • NAID: 10017973650
• [Journal Article] ナノ薬物粒子含有マイクロスフェアの設計と経肺投与製剤への応用 (2006)
  • Author(s): 尾関 哲也
  • Journal Title: 薬剤学 66・4 Pages: 249-253
• [Presentation] リファンピシン/PLGAナノ粒子含有マンニトールマイクロスフェアの設計と肺胞マクロファージ内寄生菌に対する抗菌効果 (2009)
  • Author(s): 樺澤尚宏、尾関哲也、岩崎泰憲、 鈴木寧真、吉田一平、三宅正紀、 今井康之、岡田弘晃
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都市、 京都府
  • Year and Date: 2009-03-26
• [Presentation] 結核治療用マンノース修飾PLGAナノ粒子の設計と肺胞マクロファージへの取り込み (2009)
  • Author(s): 樺澤尚宏、渡辺悦也、尾関哲也、岡田弘晃
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都市、京都府
  • Year and Date: 2009-03-26
• [Presentation] リファンピシン/PLGAナノ粒子含有マンニトールマイクロスフェアの設計と肺胞マクロファージ内寄生菌に対する抗菌効果 (2009)
  • Author(s): 樺澤 尚宏, 尾関 哲也, 岩崎 泰憲, 鈴木 寧真, 吉田一平, 三宅 正紀, 今井康之, 岡田 弘晃
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都市、京都府
  • Year and Date: 2009-03-26
• [Presentation] 経肺投与用ナノ粒子含有マイクロスフェアのワンステップによる調製とその応用 (2008)
  • Author(s): 尾関哲也、岡田弘晃
  • Organizer: 第52回日本薬学会関東支部大会
  • Place of Presentation: 野田市、千葉県
  • Year and Date: 2008-10-04
• [Presentation] Brain cancer therapy in rats using tumor-suppressor peptide, p53p-Ant (2008)
  • Author(s): Daiki Kaneko, Tetsuya Ozeki, and Hiroaki Okada
  • Organizer: 35^<th> Annual Meeting & Exposition of the Controlled Release Society
  • Place of Presentation: New York, NY, USA
  • Year and Date: 2008-07-15
• [Presentation] Pharmaceutical application of polymeric and drug nanoparticles (2008)
  • Author(s): Tetsuya Ozeki
  • Organizer: Canadian Society of Pharmaceutical Sciences (CSPS) Symposium 2008
  • Place of Presentation: Banff, Albert, Canada
  • Year and Date: 2008-05-23
• [Presentation] 結核治療用リファンピシンPLGAナノ粒子含有マイクロ粒子のin vivo蛍光イメージングによる肺内分散性の評価 (2008)
  • Author(s): 樺澤尚宏、中野麻紀子、前田千春、 尾関哲也、岡田弘晃
  • Organizer: 日本薬剤学会第23年会
  • Place of Presentation: 札幌市、北海道
  • Year and Date: 2008-05-23
• [Presentation] 結核治療用リファンピシンPLGAナノ粒子含有マイクロ粒子のinvivo蛍光イメージングによる肺内分散性の評価 (2008)
  • Author(s): 樺澤 尚宏, 中野 麻紀子, 前田 千春, 尾関 哲也, 岡田 弘晃
  • Organizer: 日本薬剤学会第23年会
  • Place of Presentation: 札幌市、北海道
  • Year and Date: 2008-05-23
• [Presentation] 結核治療用リファンピシン/PLGAナノ粒子含有マンニトールマイクロスフェアの設計と肺胞マクロファージへの取り込み評価 (2008)
  • Author(s): 樺澤尚宏、大橋克也、前田千春、尾関哲也、岡田弘晃
  • Organizer: フィジカル・ファーマフォーラム2008
  • Place of Presentation: 品川区、東京都
  • Year and Date: 2008-03-24
• [Presentation] リファンピシン/PLGAナノ粒子含有マンニトールマイクロ粒子の結核治療用経肺投与製剤としての応用 (2007)
  • Author(s): 樺澤尚宏、大橋克也、前田千春、尾関哲也、岡田弘晃
  • Organizer: 粉体工学会第24回製剤と粒子設計シンポジウム
  • Place of Presentation: 浜松市、静岡県
  • Year and Date: 2007-11-14
• [Presentation] One-step preparation of rifampicin/PLGA nanoparticles- containing microspheres using a 4-fluid nozzle spray drier for inhalation therapy of tuberculosis (2007)
  • Author(s): Katsuya Ohashi, Takuto Mizoe, Takahiro Kabasawa, Tetsuya Ozeki, and Hiroaki Okada
  • Organizer: 2007 AAPS Annual Meeting and Exposition
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2007-11-12
• [Presentation] One-step preparation of rifampicin/PLGA nanoparticles-containing microspheres using a 4-fluid nozzle spray drier for inhalation therapy of tuberculosis (2007)
  • Author(s): Katsuya Ohashi, Takuto Mizoe, Takabiro Kabasawa, Tetsuya Ozeki, and Okada Hiroaki
  • Organizer: 2007 AAPS Annual Meeting and Exposition
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2007-11-12
• [Presentation] Design of nano-, micropaticles delivery systems -Brain tumor therapy and one-step nanoparticles preparation (2007)
  • Author(s): Tetsuya Ozeki
  • Organizer: The BK21 International Symposium
  • Place of Presentation: Gwangji, Korea
  • Year and Date: 2007-09-10
• [Presentation] Design of nano-, micropaticles delivery systems-Brain tumor therapy and one-Step nanoparticles preparation (2007)
  • Author(s): Tetsuya Ozeki
  • Organizer: The BK21 International Symposium
  • Place of Presentation: Gwangji, Korea
  • Year and Date: 2007-09-10
• [Presentation] Brain cancer therapy in rats by combination use of PLGA microspheres containing anti-cancer drugs and thermoreversible gelation polymer (2007)
  • Author(s): Daiki Kaneko, Ko Tanaka, Kosuke Hashizawa, Tetsuya Ozeki, and Hiroaki Okada
  • Organizer: 34th Annual Meeting and Exposition of the Controlled Release Society
  • Place of Presentation: Long Beach, CA, USA
  • Year and Date: 2007-07-09
• [Presentation] 脳腫瘍に対するがん抑制ペプチドp53p-Antを用いた治療効果 (2007)
  • Author(s): 金子大樹、尾関哲也、岡田弘晃
  • Organizer: 日本薬剤学会第22年会
  • Place of Presentation: さいたま市、埼玉県
  • Year and Date: 2007-05-21
• [Presentation] Polymeric/drug nano- microparticles delivery systems and their pharmaceutical applications (2006)
  • Author(s): Tetsuya Ozeki, Shuji Beppu, Takuto Mizoe, Kosuke Hashizawa, Daiki Kaneko, and Hiroaki Okada
  • Organizer: The 15^<th> Nisshin Engineering Particle Technology International Seminar (NEPTIS-15)-Nano-Technology in Life Science
  • Place of Presentation: Awaji, Hyogo, Japan
  • Year and Date: 2006-12-10
• [Presentation] Polymeric/drug nanoparticles delivery systems and its pharmaceutical applications (2006)
  • Author(s): Tetsuya Ozeki
  • Organizer: International Mini-symposium for Nano-Bio Drug Delivery System
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2006-09-01
• [Presentation] ナノ・マイクロ粒子設計のためのニューテクノロジーと製剤への応用 (2006)
  • Author(s): 尾関哲也、岡田弘晃
  • Organizer: 製剤機械技術研究会第15回講演会
  • Place of Presentation: 東京都
  • Year and Date: 2006-08-25
• [Presentation] Novel nanoparticles delivery system and its pharmaceutical application (2006)
  • Author(s): Tetsuya Ozeki
  • Organizer: The 3rd Korea-Japan Joint Symposium on Drug Delivery and Therapy
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2006-04-21