| Project/Area Number |
17590080
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Shujitsu University |
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Principal Investigator |
NAKANISHI Tohru Shujitsu University, School of Pharmacy, Professor, 薬学部, 教授 (30243463)
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| Co-Investigator(Kenkyū-buntansha) |
HAYATSU Hikoya Shujitsu University, School of Pharmacy, Professor, 薬学部, 教授 (10012593)
MORI Hiroki Shujitsu University, School of Pharmacy, Assistant, 薬学部, 助手 (40388989)
OKA Tsuyoshi Okayama Univ., Graduate School of Medicine, Dentistry and Pharmaceutical Science, Assistant, 大学院医歯薬学総合研究科, 助手 (50160651)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | stem cells / telomerase / DNA chips / DNA methylation / osteoblasts / adipocytes |
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| Research Abstract |
We performed DNA microarray analysis of immortalized human mesenchymal stem cell clones. Among them, clone no.12 showed highest ability of differentiation to osteoblasts, chondrocytes and adipocytes, and its profiling analysis showed characteristic patterns of gene expression. In order to know the relationship between stem cell differentiation and DNA methylation, we next performed global methylation analysis of stem cell clones under differentiation processes by using CpG methylation array system. As a result, we isolated 11 genes under osteoblastic differentiation process and four genes under adipocytic differentiation process as candidates of up-regulated or down-regulated genes of methylation status. Furthermore, we isolated 82 genes varying their methylation levels during stem cell differentiation by using novel bisulfite modification with which genome-wide identification of methylation sites may become possible. These genes were supposed to be important for regulation of stem cell differentiation and reconstruction of methylation status. These results may contribute to the progress of stem cell research and regeneration medicine up to date.
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