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Molecular toxicological studies on individual variability in sensitivity to pyrethroid insecticides

Research Project

Project/Area Number 17590113
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental pharmacy
Research InstitutionNational Institute of Health Sciences

Principal Investigator

JINNO Hideto  National Institute of Health Sciences, Division of Environmental Chemistry, Section Chief (10179096)

Co-Investigator(Kenkyū-buntansha) KAGAWA Toshiko  National Institute of Health Sciences, Senior Researcher (40188313)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,710,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsPyrethroid insecticide / Individual variability in susceptibility / Molecular toxicological studies / 個体差 / 感受性個体差 / 分子毒性学的研究
Research Abstract

Pyrethroid insecticides are commonly used for both household and agricultural applications. To examine the role of human carboxylesterase (CES) isozymes in pyrethroid hydrolysis, we cloned the cDNAs encoding human CES1, CES2 and CES3, and expressed in HEK293 cells and characterized their hydrolytic activities.
We found that recombinant human CES1 and CES2 could hydrolyze permethrin, one of the representative type I pyrethroid, whereas, we could not detect any permethrin hydrolytic activity of CES3. These findings indicate that CES1 and CES2 play an important role in the degradation of pyrethroid insecticides in human tissues.
It has been shown that variations in the hydrolytic rates toward permethrin were observed in human liver samples. In the present study, wild-type (WT) and variant (S75N, G188R, R199H and D203E) CES1 proteins were expressed in HEK293 cells for functional characterization. WT and variant CES1s, S75N, R199H and D203E, catalyzed permethrin hydrolysis with almost similar … More apparent Km values and Vmax values. However, the hydrolysis activity of G188R was less than 5% of the wild-type CES1. These results clearly indicate that the G188R variant is essentially nonfunctional with regard to permethrin hydrolysis. These findings indicate that genetic polymorphism, G188R, could contribute to interindividual variability in metabolism of pyrethroid insecticides in human liver.
Profluthrin, one of the highly-volatile pyrethroids insecticides, is recently used for household applications. To examine the role of human CES isozymes in profluthrin hydrolysis, we characterized their hydrolytic activities. We found that recombinant human CES1 and CES2 (CES1>>CES2) can hydrolyze profluthrin, whereas, we could not detect any hydrolytic activity for CES3. It was reported that tissue specific expression of CES1 and CES2, CES1 was highly expressed in liver and lung, CES2 was highly expressed in small intestine, kidney and skin. To clarify these substrate structure-activity relationships and the tissue distribution of CES isozymes is critical for predicting the metabolism and the pharmacokinetics of pyrethroid insecticides. Less

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (14 results)

All 2008 2007 2006 2005

All Presentation (14 results)

  • [Presentation] ヒトCarbokylesteraseによる常温揮発性ピレスロイド剤の加水分解2008

    • Author(s)
      香川(田中)聡子,埴岡伸光,松本拓,徳永裕司,成松鎭雄,神野透人
    • Organizer
      日本薬学会第128年会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2008-03-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 遺伝子多型に起因するヒトCarboxylesterase1変異体のPermethrin代謝能に関する研究2007

    • Author(s)
      香川(田中)聡子,大河原晋,小濱とも子,安藤正典,徳永 裕司,神野透人
    • Organizer
      日本薬学会第127年会
    • Place of Presentation
      富山市総合体育館(富山)
    • Year and Date
      2007-03-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Permethrin代謝能に及ぼすヒトcarboxylesterase1遺伝子多型の影響2007

    • Author(s)
      香川(田中)聡子,大河原 晋,小濱とも子,徳永裕司,安藤正典,神野透人
    • Organizer
      第34回日本トキシコロジー学会 学術年会
    • Place of Presentation
      タワーホール船堀(東京)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 常温揮散性ピレスロイド剤・Profluthrinの加水分解に関与するヒトCarboxylesterase2007

    • Author(s)
      香川(田中)聡子,埴岡伸光,松本拓,徳永裕司,成松鎭雄,神野透人
    • Organizer
      フォーラム2007:衛生薬学・環境トキシコロジー
    • Place of Presentation
      アピオ大阪森の宮ピロティーホール(大阪)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effect of genetic polymorphism of human carboxylesterase 1 on the permethrin metabolism.2007

    • Author(s)
      Toshiko Tanaka-Kagawa, Susumu Ohkawara, Tomoko Obama, Masanori Ando, Hiroshi Tokunaga Hideto Jinno
    • Organizer
      The 34th Annual Meeting of the Japanese Society of Toxicology
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ヒトCarboxylesteraseによる家庭用殺虫剤ペルメトリンの加水分解2006

    • Author(s)
      香川(田中)聡子,神野透人,大河原晋,安藤正典,徳永裕司
    • Organizer
      日本薬学会第126年会
    • Place of Presentation
      せんだいメディアパーク(仙台)
    • Year and Date
      2006-03-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Hydrolysis of domestic insecticide permethrin by human carboxylesterases.2006

    • Author(s)
      Toshiko Tanaka-Kagawa, Hideto Jinno, Susumu Ohkawara, Masanori Ando, Hiroshi Tokunaga
    • Organizer
      The 126th Annual Meeting of the Pharmaceutical Society of Japan
    • Place of Presentation
      Sendai
    • Year and Date
      2006-03-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effect of genetic polymorphism of human carboxylesterase 1 on the hydrolysis of Permethrin.2006

    • Author(s)
      Toshiko Tanaka-Kagawa, Susumu Ohkawara, Masanori Ando, Hiroshi Tokunaga Hideto Jinno
    • Organizer
      The 127th Annual Meeting of the Pharmaceutical Society of Japan
    • Place of Presentation
      Toyama
    • Year and Date
      2006-03-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Hydrolysis of the highly-volatile pyrethroid insecticides by human carboxylesterases.2006

    • Author(s)
      Toshiko Tanaka-Kagawa, Susumu Ohkawara, Tomoko Obama, Masanori Ando, Hiroshi Tokunaga Hideto Jinno
    • Organizer
      The 128th Annual Meeting of the Pharmaceutical Society of Japan
    • Place of Presentation
      Yokohama
    • Year and Date
      2006-03-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ピレスロイド系殺虫剤の代謝に及ぼすヒトCarboxylesterase遺伝子多型の影響2006

    • Author(s)
      香川(田中)聡子,大河原晋,小濱とも子,安藤正典,徳永裕司,神野透人
    • Organizer
      フォーラム2006:衛生薬学・環境トキシコロジー
    • Place of Presentation
      アルカディア市ケ谷(東京)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ピレスロイド系殺虫剤の加水分解に関与するヒトCarboxylesterase2005

    • Author(s)
      香川(田中)聡子,神野透人,大河原晋,安藤正典,徳永裕司
    • Organizer
      フォーラム2005:衛生薬学・環境トキシコロジー
    • Place of Presentation
      徳島県郷土文化会館(徳島)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Hydrolysis of pyrethroid insecticides by human carboxylesterases.2005

    • Author(s)
      Toshiko Tanaka-Kagawa, Hideto Jinno, Susumu Ohkawara, Masanori Ando, Hiroshi Tokunaga
    • Organizer
      Forum 2005 : Pharmaceutical Health Science and Environmental Toxicology
    • Place of Presentation
      Tokushima
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effect of genetic polymorphism of human carboxylesterase 1 on the hydrolysis of pyrethroid insecticides.2005

    • Author(s)
      Toshiko Tanaka-Kagawa, Susumu Ohkawara, Tomoko Obama, Masanori Ando, Hiroshi Tokunaga Hideto Jinno
    • Organizer
      Forum 2006 : Pharmaceutical Health Science and Environmental Toxicology
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Hydrolysis of profluthrin, the highly-volatile pyrethroid insecticides, by human carboxlesterases.2005

    • Author(s)
      Toshiko Tanaka-Kagawa, Nobumitsu Hanioka, Taku Matsumoto, Hiroshi Tokunaga, Shizuo Narimatsu, Hideto Jinno
    • Organizer
      Forum 2007 : Pharmaceutical Health Science and Environmental Toxicology
    • Place of Presentation
      Osaka
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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