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Functional Analyses of Protocadherin 10 in Embryonic Development of Central Nervous System and Paraxial Mesoderm

Research Project

Project/Area Number 17590147
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionGunma University

Principal Investigator

MURAKAMI Tohru  Gunma University, Graduate School of Medicine, Assistant Professor, 大学院医学系研究科, 講師 (10239494)

Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordscadherin / protocadherin / central nervous system / somite / pharyngeal arch / pronephros / zebrafish / Morpholino / ゼブラフィッシュ / 沿軸中胚葉 / 向軸細胞 / レンズ
Research Abstract

The investigator and collaborators studied biological functions of Protocadherin-lO (Pcdh1O) and other cadherins in zebrafish development. In situ hybridization (ISH) showed that pcdh1Ob was expressed in the paraxial mesoderm (PAM) and developing somites, and in the pineal body, the diencephalon and the vicinity of otocysts. Expression in PAM and somites decreased during somite maturation. A dominant-negative form of Pcdh1Ob and anti-sense Morpholino of pcdh1Ob disturbed convergence of PAM and somite segmentation. Comparative studies showed that differential expressions of pcdh1Ob and pcdh8 in PAM and the adaxial. We propose that Pcdh1Ob is involved in the convergence of PAM and somite segmentation, and that differential adhesion of Pcdh8 and Pcdh10b plays a role in the morphogenic machinery of somites and adaxial cells.
We performed functional analyses of cadherin-6 (cdh6) in zebrafish nephrogenesis using antisense morpholino oligonucleotide (MO) inhibition combined with ISH. We cloned … More a zebrafish homolog of human K-cadherin. Whole-mount ISH showed that cdh6 is expressed in the pronephric ducts and nephron primordium in addition to the central and peripheral nervous systems. Embryos injected with MOs directed against cdh6 (cdh6MOs) showed developmental defects including small head, body axis curvature, short yolk extension, and short bent tail by 30 hpf, and edema appeared in the thorax by 42 hpf. Embryos injected with cdh6MOs were subjected to in situ hybridization with pronephric markers, wtl and pax2.1, to examine disturbed development of the anterior region of the pronephric ducts and the nephron primordia. Histological studies showed malformation of the pronephros as abnormally fused glomerulus primordia, fused or abnormally bent pronephric tubule anlage, and thin pronephric ducts. These results suggested that cdh6 plays pivotal roles in development of the pronephros in zebrafish embryos.
We also studied zebrafish Protocadherin-18. It was expressed central nervous system, pharyngeal arches and gut primodia. The results are currently to be compiled as a scientific report. Less

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (14 results)

All 2007 2006 Other

All Journal Article (11 results) Book (3 results)

  • [Journal Article] Cadherin-6 is required for zebrafish nephrogenesis during early development2007

    • Author(s)
      久保田文隆ほか
    • Journal Title

      Int J Dev Biol 51

      Pages: 123-129

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] VAMP2 is expressed in muscle satellite cells and up-regulated during muscle regeneration2007

    • Author(s)
      多鹿友喜ほか
    • Journal Title

      Cell and Tissue Research 328

      Pages: 573-581

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 新しい蛍光プローブの使い方2007

    • Author(s)
      村上 徹, 多鹿友喜
    • Journal Title

      顕微鏡 42

      Pages: 65-68

    • NAID

      10018913028

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Cadherin-6 is required for zebrafish nephrogenesis during early development2007

    • Author(s)
      Kubota, F.et al.
    • Journal Title

      International Journal of Developmental Biology 51

      Pages: 123-129

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cell biological applications of new fluorescent probes2007

    • Author(s)
      Murakami, T., Tajika, Y.
    • Journal Title

      KENBIKYO 42

      Pages: 65-68

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] VAMP2 is expressed in muscle satellite cells and up-regulated during muscle regeneration2007

    • Author(s)
      多鹿友喜ほか
    • Journal Title

      Cell and Tissue Research (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Zebrafish protocadherin 10 is involved in paraxial mesoderm development and somitogenesis2006

    • Author(s)
      村上 徹, ほか
    • Journal Title

      Developmental Dynamics 235

      Pages: 506-514

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Zebrafish protocadherin 10 is involved in paraxial mesoderm development and somitogenesis2006

    • Author(s)
      Murakami, T.et al.
    • Journal Title

      Developmental Dynamics 235

      Pages: 506-514

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Zebrafish protocadherin 10 isinvolved in paraxial mesoderm development and somitogenesis2006

    • Author(s)
      村上 徹, ほか
    • Journal Title

      Developmental Dynamics 235

      Pages: 506-514

    • Related Report
      2005 Annual Research Report
  • [Journal Article] ゼブラフィッシュの体節形成におけるプロトカドヘリン10の役割2006

    • Author(s)
      村上 徹, ほか
    • Journal Title

      Acta Anatomica Nipponica 81(Suppl)

      Pages: 208-208

    • Related Report
      2005 Annual Research Report
  • [Journal Article] VAMP2 is expressed in muscle satellite cells and up-regulated during muscle regeneration

    • Author(s)
      Tajika, Y.et al.
    • Journal Title

      Cell and Tissue Research

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 染色・バイオイメージンブハンドブック2006

    • Author(s)
      高田邦昭ほか
    • Total Pages
      335
    • Publisher
      羊土社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] SENSHOKU-Bio-Imaging Handbook2006

    • Author(s)
      Takata K.Ed.
    • Total Pages
      335
    • Publisher
      YODOSHA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 染色・バイオイメージングハンドブック2006

    • Author(s)
      高田邦昭ほか
    • Total Pages
      335
    • Publisher
      羊土社
    • Related Report
      2006 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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