The Molecular Mechanism Involved in the Sensory Axonal Projection toward the Spinal Cord
| Project/Area Number |
17590166
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
MASUDA Tomoyuki Fukushima Medical University, School of Medicine ,Department of Anatomy, Assistant Professor (70372828)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Kenta Fukushima Medical University School of Medicine, Department of Molecular Genetics, Assistant Professor (70315662)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | axon guidance / spinal cord / DNA microarray / laminin |
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| Research Abstract |
During early development, centrally projecting dorsal root ganglion (DRG) neurons extend their axons toward the dorsal spinal cord. We found the involvement of dorsal spinal cord-derived chemoattraction in this projection. However, the molecular nature of this attraction is not clear. We showed that laminin-1 (α1β1γ1) is expressed strongly along the pathway of DRG axons and that its 67-kDa receptor (67LR) was present on DRG cells. By employing culture assays, we showed that laminin-1 or the YIGSR peptide, a soluble peptide of the laminin β1 chain, promoted the DRG axonal response to dorsal spinal cord-derived chemoattraction. By using a function-blocking antibody against 67LR, we showed that the anti-67LR antibody blocked the modulation of DRG axonal response by the YIGSR peptide in vitro. Furthermore, the in ovo injection of the anti-67LR antibody inhibited the DRG axonal growth, toward the dorsal spinal cord. These results provide evidence that the YIGSR peptide promotes dorsal spinal cord-derived chemoattraction via 67LR to contribute to the formation of the initial trajectories of developing DRG axons. Next, to identify novel genes differentially expressed in an early dorsal spinal cord, we used the Kazusa cDNA array system and laser capture microdissection. We observed that a certain population of genes showed significantly increased expression in the dorsal spinal cord.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] Guidance cues from the embryonic dorsal spinal cord chemoattract dorsal root ganglion axons2007
Author(s)
Tomoyuki, Masuda, Chie, Sakuma, Masahiko, Taniguchi, Kenta, Kobayashi, Kazuto Kobayashi, Takashi, Shiga, Hiroyuki, Yaginuma
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Journal Title
Neuroreport 18
Pages: 1645-1649
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Identification and characterization of zebrafish semaphorin 6D2007
Author(s)
Masahiko, Taniguchi, Masafumi, Kimura, Yoshinori, Mikami, Tomoyuki, Masuda, Tomoyuki, Yoshida, Masayoshi, Mishina, Takao, Shimizu
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Journal Title
Biochemical and Biophysical Research Communications 363
Pages: 762-768
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification and characteraization of a novel member of murine semaphorin family2005
Author(s)
Masahiko, Taniguchi, Tomoyuki, Masuda, Masahiro, Fukaya, Hirotaka, Kataoka, Masami, Mishina, Hiroyuki, Yaginuma, Masahiko, Watanabe, Takao, Shimizu
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Journal Title
Genes to Cells 10
Pages: 785-792
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Laminin peptide YIGSR and its receptor regulate sensory axonal response to the chemoattractive guidance cue in the chick embryo
Author(s)
Tomoyuki, Masuda, Chie, Sakuma, Kenta, Kobayashi, Kazufumi, Kikuchi, Emi, Soda, Takashi, Shiga, Kazuto, Kobayashi, Hiroyuki, Yaginuma
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Journal Title
Journal of Neuroscience Research (in press)
Description
「研究成果報告書概要(欧文)」より
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[Book] Neural Proteoglycans2007
Author(s)
Tomoyuki Masuda, Takshi Shiga
Total Pages
11
Publisher
Roles of chondroitin sulfate proteoglycans in the formation of axonal trajectories of dorsal root ganglion neurons
Description
「研究成果報告書概要(和文)」より
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