| Project/Area Number |
17590166
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
MASUDA Tomoyuki Fukushima Medical University, School of Medicine ,Department of Anatomy, Assistant Professor (70372828)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Kenta Fukushima Medical University School of Medicine, Department of Molecular Genetics, Assistant Professor (70315662)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | axon guidance / spinal cord / DNA microarray / laminin |
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| Research Abstract |
During early development, centrally projecting dorsal root ganglion (DRG) neurons extend their axons toward the dorsal spinal cord. We found the involvement of dorsal spinal cord-derived chemoattraction in this projection. However, the molecular nature of this attraction is not clear. We showed that laminin-1 (α1β1γ1) is expressed strongly along the pathway of DRG axons and that its 67-kDa receptor (67LR) was present on DRG cells. By employing culture assays, we showed that laminin-1 or the YIGSR peptide, a soluble peptide of the laminin β1 chain, promoted the DRG axonal response to dorsal spinal cord-derived chemoattraction. By using a function-blocking antibody against 67LR, we showed that the anti-67LR antibody blocked the modulation of DRG axonal response by the YIGSR peptide in vitro. Furthermore, the in ovo injection of the anti-67LR antibody inhibited the DRG axonal growth, toward the dorsal spinal cord. These results provide evidence that the YIGSR peptide promotes dorsal spinal cord-derived chemoattraction via 67LR to contribute to the formation of the initial trajectories of developing DRG axons. Next, to identify novel genes differentially expressed in an early dorsal spinal cord, we used the Kazusa cDNA array system and laser capture microdissection. We observed that a certain population of genes showed significantly increased expression in the dorsal spinal cord.
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