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Role of Homeobox transcription factor encoding gene, Six in peripheral neural network

Research Project

Project/Area Number 17590172
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionJichi Medical University

Principal Investigator

YAMAKADO Makoto  Jichi Medical University, Research associate, 医学部, 研究員 (80010114)

Co-Investigator(Kenkyū-buntansha) IKEDA Keiko  Jichi Medical University, Associate Professor, 医学部, 准教授 (10265241)
KAWAKAMI Kiyoshi  Jichi Medical University, Professor, 医学部, 教授 (10161283)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsSix1 / Six4 / Neurogenin / Sox10 / apoptosis / epibranchial ganglion / placode / neural crest / Neurogenin / Six / 遺伝子欠損マウス / 脳神経節
Research Abstract

Six genes are homologues of the Drosophila sine oculis (so), which plays an essential role in the development of compound eye. There are six members of Six genes, from Six1 to Six6 in mice. Of these, Six1 and Six4 are expressed in sensory placodes, trigeminal ganglion, epibranchial ganglia, and neural crest cells during mouse development. To understand roles of Six genes in formation of sensory peripheral nervous system, we analyzed double homozygous knockout mice (Six1^<-/->Six4^<-/->). During the development of placode-derived and neural crest-derived cranial sensory ganglia, an early arrest of neurogenesis was observed in Six1^<-/->Six4^<-/->. The epibranchial progenitor cells failed to express Neurogenin1 and Neurogenin2, that are normally expressed and required for the determination of neuronal precursors in placodal-derived trigeminal and epibranchial ganglion, respectively at embryonic day (E) 9.5. NeuroD as well as Phox2b genes that are essential for neural differentiation and maintenance, were also dramatically decreased. Moreober, failure to activate normal differentiation program in placodal-derived neurons resulted in abnormal apoptosis of the progenitor cells in Six1^<-/->Six4^<-/->. We also observed that the delamination of placode epithelial cells to form ganglia were disturbed in Six1^<-/->Six4^<-/->. As for neural crest cell, the expression of Sox10 at E8.5 was not altered in wild-type and Six1^<-/->Six4^<-/->, while its expression at E10.5 was severely reduced in Six1^<-/->Six4^<-/->. These results indicated that production and migration of neural crest cells were not disturbed, but differentiation to glial cells were disrupted in Six1^<-/->Six4^<-/->.In sum, our data suggest that Six1 and Six4 play roles for early differentiation, delamination, and survival of the placodally derived cranial sensory neurons and differentiation of neural crest cells.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (11 results)

All 2007 2006 2005

All Journal Article (11 results)

  • [Journal Article] Six1 and Six4 are essential for Gdnf expression in the metanephric mesenchyme and ureteric bud formation, while Six1 deficiency alone causes mesonephric tubule defects.2007

    • Author(s)
      Kobayashi, H.
    • Journal Title

      Mech. Dev. 124

      Pages: 290-303

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Six1 and Six4 are essential for Gdnf expression in the metanephric mesenchyme and ureteric bud formation, while Six1 deficiency alone causes mesonephric tubule defects.2007

    • Author(s)
      Kobayashi, H., et al.
    • Journal Title

      Mech.Dev 124

      Pages: 290-303

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Six1 and Six4 are essential for Gdnf expression in the metanephric mesenchyme and ureteric bud formation, while Six1 deficiency alone causes mesonephric tubule defects.2007

    • Author(s)
      Kobayashi, H.
    • Journal Title

      Mech.Dev. 124

      Pages: 290-303

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Modulation of neural activities by Na,K・ATPase a α2 subunit through functional coupling with transporters.2007

    • Author(s)
      Kawakami, K.
    • Journal Title

      Cellular and Molecular Biology 52

      Pages: 88-92

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Six1 and Six4 promote survival of sensory neurons during early trigeminal eangliogenesis.2006

    • Author(s)
      Konishi, Y.
    • Journal Title

      Brain Res. 1116

      Pages: 93-102

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Six1 and Six4 promote survival of sensory neurons during early trigeminal gangliogenesis.2006

    • Author(s)
      Konishi, Y., et al.
    • Journal Title

      Brain Res. 1116-1

      Pages: 93-102

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Transcriptional activation of the SALL1 by the human SIX1 homeodomain during kidney developmento.2006

    • Author(s)
      Chai, L.
    • Journal Title

      J.Biol.Chem. 281

      Pages: 18918-18926

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Six1 and Six4 promote survival of sensory neurons during early trigeminal gangliogenesis.2006

    • Author(s)
      Konishi, Y.
    • Journal Title

      Brain Res. 1116

      Pages: 93-102

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Divergent signaling pathways mediate induction of Na,K・ATPaseal and B1 subunit gene transcription by low potassium.2006

    • Author(s)
      Wang, G.
    • Journal Title

      Mol.Cell.Biochem. 294

      Pages: 73-85

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Slc12a2 is a direct target of two closely related homeobox proteins, Six1 and Six4.2005

    • Author(s)
      Ando, Z.
    • Journal Title

      FEBS J. 273

      Pages: 3026-3041

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Hyperphagia and Obesity in Na, K-ATPase alpha2 Subunit Defective Mice.2005

    • Author(s)
      Kawakami, K.
    • Journal Title

      Obesity Res. 13

      Pages: 1661-1671

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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