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Identfication of novel genes highly expreseed in the mitotic neural progenitor/stem cells

Research Project

Project/Area Number 17590173
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionDokkyo Medical University

Principal Investigator

SAKAKIBARA Shin-ichi  Dokkyo Medical University, Deparment of Histology & Neurobiology, Associate Professor (70337369)

Co-Investigator(Kenkyū-buntansha) UEDA Shuichi  Dokkyo Medical Univenrity, Department of Histology & Neurobiology, Professor (60150570)
NAKADATE Kazuhiko  Dokkyo Medical Universtiy, Department of Histology & Neurobiology, Associate Professor (80372895)
野田 隆洋  獨協医科大学, 医学部, 助手 (90364596)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsneural stem cell / Radmps / mitotic spindle / microtubules / neural progenitor cell / central nervous system / neurogenesis
Research Abstract

Identification of the genes regulating neural progenitor neural stem cell functions is critical to understand the mechanisms of the adult neurogenesis and neurodegenerative disease. We compared the gene expression profile of proliferating neural stem cell culture with those of differentiated cells, and identified a novel gene named "Radmis (radial fiber associated mitotic spindle protein)". Northern blot analysis indicated radmis mRNA is expressed in the neural stem cells in vitro. In the developing and matured CNS, radmis protein was highly enriched in the mitotic neural progenitors. At E11 and E13 developing cerebral cortex, radmis expression was observed in the mitotic spindle, and radial fiber of the nestin-positive neuroepithelial cells, neural stem cells, located at the ventricular surface. At early postnatal CNS, radmis is expressed in positive neural progenitor cells in the SVZ of lateral ventricles, and 4th ventricle. In the developing cerebellum at P6, radmis is expressed in … More the mitotic spindle of the proliferating neuronal progenitor cells within the EGL layer, the superficial layer of cerebellum, but it was absent from the NeuN-positive post-mitotic neurons in both EGL arid IGL, the inner layer of the cerebellum. Radmis expression is drastically decreased as the CNS development proceeds, but a small number of cells in SVZ neurogenic region remained to express radmis protein. In the adult SVZ surrounding the lateral ventricles, radmis is expressed in the dividing neural stem cells (type B cells) that are positive for GFAP, and also in the proliferating neuronal progenitors (rapid amplifying cells, type C cells) that are positive for nestin. During the cell cycle, radmis protein did not expressed in the microtubules in interphase cells. At prometaphase, metaphase and anaphase, radmis colocalized with mitotic spindle poles and spindle microtubules proximal to the centrosomes, suggesting that radmis may control the spindle structure and function during the embryonic and adult neurogenesis. Less

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (37 results)

All 2008 2007 2006 2005 Other

All Journal Article (18 results) (of which Peer Reviewed: 9 results) Presentation (16 results) Book (3 results)

  • [Journal Article] Attractin/Mahogany protein expression in the rodent central nervous system2008

    • Author(s)
      Nakadate K, Sakakibara S, Ueda S
    • Journal Title

      J.Comp.Neurol 508

      Pages: 94-111

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Non-Cell autonomous Impairment of Oligodendrocyte Diffenrentiation precedes CNS Degeneration in the utter Rat:Implications of Macrophage/Microglial Activation in the Pathogenesis2008

    • Author(s)
      Sakakibara S, Nakadate K, Ookawara S, Ueda S
    • Journal Title

      BMC Neuroscience 9

      Pages: 35-35

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Attractin/Mahogany protein expression in the rodent central nervous system2008

    • Author(s)
      Nakadate K, Sakakibara S, Ueda, S
    • Journal Title

      J. Comp. Neurol 508

      Pages: 94-111

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Non-Cell autonomous Impairment of Oligodendrocyte Differentiation precedes CNS Degeneration in the zitter Rat : Implications of Macrophage/Microglial Activation in the Pathogenesis2008

    • Author(s)
      Sakakibara S, Nakadate K, Ookawara S, Ueda, S
    • Journal Title

      BMC Neuroscience 9, 35

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Non-Cell autonomous Impairment of Oligodendrocyte Differentiation precedes CNS Degeneration in the zitter Rat:Implications of Macrophage/Microglial Activation in the Pathogenesis2008

    • Author(s)
      Sakakibara S, Nakadate K, Ookawara S, Ueda S
    • Journal Title

      BMC Neuroscience (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of Ibal protein in microglial cells of zitter mutant rat2007

    • Author(s)
      Kadowaki T, Nakadate K, Sakakibara S, Hirata K, Ueda S
    • Journal Title

      Neurosci.Lett 411

      Pages: 26-31

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Expression of Iba1 protein in microglial cells of zitter mutant rat2007

    • Author(s)
      Kadowaki T, Nakadate K, Sakakibara S, Hirata K, Ueda, S
    • Journal Title

      Neurosci. Lett 411

      Pages: 26-31

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Expression of Iba1 protein in microglial cells of zitter mutant rat2007

    • Author(s)
      Kadowaki T, Nakadate K, Sakakibara S, Hirata K, Ueda S
    • Journal Title

      Neurosci.Lett 411

      Pages: 26-31

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Progressive dopaminergic neurodegeneration of substantia nigra in the zitter mutant rat2006

    • Author(s)
      Kadowaki T, Nakadate K, Sakakibara S, Hiiata K, Ueda S
    • Journal Title

      Acta Neuropathol 112

      Pages: 64-73

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Progressive dopaminergic neurodegeneration of substantia nigra in the zitter mutant rat2006

    • Author(s)
      Kadowaki T, Nakadate K, Sakaibara, S Hirata K, Ueda, S
    • Journal Title

      Acta. Neuropathol 112

      Pages: 64-73

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Progressive dopaminergic neurodegeneration of substantia nigra in the zitter mutant rat2006

    • Author(s)
      Nakadate khosi, Noda T, Sakakibara S, Kumamoto K, Matsuura T, Joice JN, ueda S
    • Journal Title

      Acta.Neuropathol 112.64

      Pages: 64-73

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Expression of Ibal protein in microglial cells of zitter mutant rat2006

    • Author(s)
      Kadowaki T, Nakadate K, Sakakibara S, Ueda S
    • Journal Title

      Neurosci Lett 411

      Pages: 26-31

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The RNA-binding protein HuD regulates neuronal cell identity and maturation2005

    • Author(s)
      Akamatsu W, Fujihara H, Mitsuhashi T, Yano M, Shibata S, Hayakawa Y, Okano HJ, Sakakibara S, Takano H, Takana T, Takahashi T, Node T, Okano H
    • Journal Title

      Proc Natl Acad Sci USA 102

      Pages: 4625-4630

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effect of long-lasting serotonin depletion on environmental enrichmentinduced neurogenesis in adult rat hippocampus and spatial learning2005

    • Author(s)
      Ueda S, Sakakibara S, Yoshimoto K.
    • Journal Title

      Neuroscience 135

      Pages: 395-402

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Degeneration of dopaminergic neurons in the substantia nigra of zitter mutantrat and protection by chronic intake of Vitamin E2005

    • Author(s)
      Ueda S, Sakakibara S, Nakadate K, Noda T, Shinoda M, Joyce JN
    • Journal Title

      Neumosci.Lett 380

      Pages: 252-356

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Degeneration of dopaminergic neurons in the substantia nigra of zitter mutant rat and protection by chronic intake of Vitamin E2005

    • Author(s)
      Ueda S, Sakakibara S, Nakadate K, Node T, Shinoda M, Joyce, JN
    • Journal Title

      Neuroaci. Lett 380

      Pages: 252-356

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The RNA-binding protein HuD regulates neuronal cell identity and maturation2005

    • Author(s)
      Akamatsu, W, Fujihara H, Mitsuhashi T, Yano M, Shibata S, Hayakawa Y, Okano, HJ, Sakakibara S, Takano H, Takano T, Takahashi T, Noda T, Okano, H
    • Journal Title

      Proc Natl Aced Sci USA 102

      Pages: 4625-4630

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effect of long-lasting serotonin depletion on environmental enrichment-induced neurogenesis in adult rat hippocampus and spatial learning2005

    • Author(s)
      Ueda S, Sakakibara S, Yoahimoto, K
    • Journal Title

      Neuroscience 135

      Pages: 395-402

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] zrtterラット脳におけるオリゴデンドロサイトの分化異常とマクロファージ/ミクログリア活性化2008

    • Author(s)
      榊原 伸一、中舘 和彦、上田 秀一
    • Organizer
      第113回日本解剖学会学術集会
    • Place of Presentation
      大分
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] zitterラット脳におけるオリゴデンドロサイトの分化異常とマクロファージ/ミクログリア活性化2008

    • Author(s)
      榊原 伸一、中舘 和彦、上田 秀一
    • Organizer
      日本解剖学会学術集会
    • Place of Presentation
      San Diego,USA
    • Related Report
      2007 Annual Research Report
  • [Presentation] Identification of novel genes highly expressed in the mitotic neural proge nitor/stem cells.Expression of a mitotic spindle protein Radmis in neural progenitor cells2007

    • Author(s)
      Sakakibara S
    • Organizer
      New York neural Stem Cell Institute Symposium
    • Place of Presentation
      New York,USA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of a mitotic spindle protein Radmis in neural progenitor cells2007

    • Author(s)
      Sakakibara S, Nakadate K, Ueda S
    • Organizer
      Neuroscience 2007, Annual meeting of Society for Neuroscience
    • Place of Presentation
      San Diego,USA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] RNA結合蛋白質Musashiの哺乳類中枢、末梢神経系の発生、再生過程における解析2007

    • Author(s)
      芝田 晋介, 榊原 伸一, 岡野 ジェイムス洋尚, 岡野 栄之
    • Organizer
      Neuro2007第30回日本神経科学大会
    • Place of Presentation
      横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 神経前駆細胞における新規遺伝子Radmisの発現2007

    • Author(s)
      榊原 伸一、中舘 和彦、大桃 秀樹、小川 哲郎、上田 秀一
    • Organizer
      第112回日本解剖学会全国学術集会
    • Place of Presentation
      大阪
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of a mitotic spindle protein Radmis in neural progenitor cells2007

    • Author(s)
      Sakakibara S, Nakadate K, Ueda, S
    • Organizer
      Neuroscience 2007, Annual meeting of Society for Neuroscience
    • Place of Presentation
      San Diego, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of a mitotic spindle protein Radmis in neural pmgenitor cells2007

    • Author(s)
      Sakakibara S, Nakadate K, Ueda S
    • Organizer
      Neuroscience 2007, Annual meeting of Society for Neuroscience
    • Place of Presentation
      San Diego,USA
    • Related Report
      2007 Annual Research Report
  • [Presentation] Identification of novel genes highly expressed in the mitotic neural progenitor/stem cells.Expression of a mitotic spindle protein Radmis in neural progenitor cells2007

    • Author(s)
      Sakakibara S
    • Organizer
      New York neural Stem Cell Institute Symposium
    • Place of Presentation
      New York,USA
    • Related Report
      2007 Annual Research Report
  • [Presentation] 神経前駆細胞に発現する新規タンパク質の同定2006

    • Author(s)
      榊原 伸一、中舘 和彦、上田 秀一
    • Organizer
      第29回日本神経科学大会
    • Place of Presentation
      京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Identification of a novel gene Radmis in the mitotic neural progenitor cells2006

    • Author(s)
      Sakakibara S, Nakadate K, Ueda S
    • Organizer
      Neuroscience 2006, annual meeting of Society for Neuroscience
    • Place of Presentation
      Atlanta,Georgia,USA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Identification of a novel gene Radmis in the mitotic neural progenitor cells2006

    • Author(s)
      Sakakibara S, Nakadate K, Ueda, S
    • Organizer
      Neuroscience 2006, Annual meeting of Society for Neuroscience
    • Place of Presentation
      Atlanta, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 神経分化に関わる新規遺伝子の探索2005

    • Author(s)
      榊原 伸一、野田 隆洋、中舘 和彦、上田 秀一
    • Organizer
      第37回日本臨床分子形態学会総会シンポジウ
    • Place of Presentation
      大阪
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Regulatory mechanisms for the proliferation and maintenance of neural progenitor/stem cell populations2005

    • Author(s)
      Sakakibara S, Nakadate K, Noda T, Ueda S
    • Organizer
      16th International Congress of the IFAA
    • Place of Presentation
      Kyoto,Japan
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Regulatory mechanisms for the proliferation and maintenance of neural progenitor/stem cell populations2005

    • Author(s)
      Sakakibara S, Nakadate K, Node T, Ueda, S
    • Organizer
      16th International Congress of the IFAA, Aug
    • Place of Presentation
      Kyoto, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Identification of novel genes highly expressed in the mitotic neural progenitor/stem cells

    • Author(s)
      Sakakibara, S
    • Organizer
      New York Neural Stem Cell Institute Seminar
    • Place of Presentation
      Albany, NY, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] ブレインサイエンスレビュー2007 「神経系幹細胞に発現する遺伝子群の解析」2007

    • Author(s)
      伊藤 正男, 川合 述史編
    • Total Pages
      238
    • Publisher
      クバプロ
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] ブレインサイエンスレビュー2007「神経系幹細胞に発現する遺伝子群の解析」2007

    • Author(s)
      伊藤 正男, 川合 述史編
    • Total Pages
      238
    • Publisher
      クバプロ
    • Related Report
      2007 Annual Research Report
  • [Book] 神経系幹細胞に発現する遺伝子群の解析2007

    • Author(s)
      伊藤 正男 川合 述史編
    • Total Pages
      238
    • Publisher
      クバプロ
    • Related Report
      2006 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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