Research Project
Grant-in-Aid for Scientific Research (C)
Population of multi-hormonal cells were detected in rat anterior pituitary gland, using primary culture of dispersed pituitary cells.1. The double-labeling method was optimized to detect multi-hormonal cells in pituitary primary culture. Colocalization of mRNA and hormone protein of LH beta and GH was used as a model. Regarding to both sensitivity and specificity, sandwich labeling method with antibodies conjugated with HRP polymer or with biotin can be used to this study. Immunofluorescent labeling with TSA intensification was also good method for automated image analysis to count double labeled cells.2. Pituitary cells producing LH and GH were detected in pituitary dispersion from normal cycling and ovariectomized female rats. In the rats 1 week after ovariectomized, the population of LH/GH double-labeled cells was higher than that of proestrous rats. Particularly, LH beta mRNA-positive ratio of GH protein-positive cells was significantly different It is suggested that GnRH may be concerned with the appearance of LH/GH cells.3. Double-positive cells for transcription factor Pit-1 and LH beta mRNA or protein were also detected. The ratio of appearance of those cells was higher in pituitary dispersion from proestrous rats than from diestrous rats. Interestingly, the percentage of Pit-1/LH cells to total pituitary cells was lower than expected by that of GH/LH cells, suggesting only a portion of LH/GH cells is Pit-1 positive. This should be clarified in the further study.
All 2008 2007 2006 2005
All Journal Article (18 results) (of which Peer Reviewed: 6 results) Presentation (23 results) Book (4 results)
Journal of Histochemistry and Cytochemistry 55
Pages: 151-166
Basic Research in Cardiology 102
Pages: 154-162
Pages: 1059-1073
In : Histocytochemistry 2007 - from the basic to trend technology. Ed by The Japan Society of Histochemistry and Cytochemistry. Gakusai-Kikaku, Tokyo
Pages: 181-190
J Histochem Cytochem 55
Journal of Histochemistry and Cytochemistry 55・2
Basic Research in Cardiology 102・2
Nephron Physiology 104
Pages: 87-94
Kidney International 69
Pages: 1350-1359
In : Handbook of staining and bioimaging experiments : Eds by Takata K, Saito N, Kawakami H, Yodo-sha, Tokyo
Pages: 293-302
In : Handbook of staining and bioimaging experiments : . Eds by Takata K, Saito N, Kawakami H, Yodo-sha, Tokyo
Pages: 303-311
Nephron Physiology 104・2
Kidney International 69・8
