Population of Multi-hormonal cells in the anterior pituitary gland
| Project/Area Number |
17590175
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kyorin University |
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Principal Investigator |
KUDO Akihiko Kyorin University, Faculty of Medicine, Assistant Professor (80255398)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | tissues and cells / hormone / pituitary gland / gene expression / cell differentiation / international communication / U. S. A. / 細胞・組織 |
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| Research Abstract |
Population of multi-hormonal cells were detected in rat anterior pituitary gland, using primary culture of dispersed pituitary cells.
1. The double-labeling method was optimized to detect multi-hormonal cells in pituitary primary culture. Colocalization of mRNA and hormone protein of LH beta and GH was used as a model. Regarding to both sensitivity and specificity, sandwich labeling method with antibodies conjugated with HRP polymer or with biotin can be used to this study. Immunofluorescent labeling with TSA intensification was also good method for automated image analysis to count double labeled cells.
2. Pituitary cells producing LH and GH were detected in pituitary dispersion from normal cycling and ovariectomized female rats. In the rats 1 week after ovariectomized, the population of LH/GH double-labeled cells was higher than that of proestrous rats. Particularly, LH beta mRNA-positive ratio of GH protein-positive cells was significantly different It is suggested that GnRH may be concerned with the appearance of LH/GH cells.
3. Double-positive cells for transcription factor Pit-1 and LH beta mRNA or protein were also detected. The ratio of appearance of those cells was higher in pituitary dispersion from proestrous rats than from diestrous rats. Interestingly, the percentage of Pit-1/LH cells to total pituitary cells was lower than expected by that of GH/LH cells, suggesting only a portion of LH/GH cells is Pit-1 positive. This should be clarified in the further study.
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Report
(4 results)
Research Products
(45 results)
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[Journal Article] Anterior pituitary leptin expression changes in different reproductive states : in vitro stimulation by gonadotropin releasing hormone2007
Author(s)
Akhter, N, Johnson, BW, Crane, C, Iruthayanathan, M, Zhou, YH, Kudo, A, Childs, GV
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Journal Title
Journal of Histochemistry and Cytochemistry 55
Pages: 151-166
Description
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[Journal Article] Endothelin receptors, localized in sympathetic nerve terminals of the heart, modulate norepinephrine release and reperfusion arrhythmias2007
Author(s)
Isaka, M, Kudo, A, Imamura, M, Kawakami, H, Yasuda, K
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Journal Title
Basic Research in Cardiology 102
Pages: 154-162
Description
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[Journal Article] Fasting and glucose effects on pituitary leptin expression : is leptin a local signal for nutrient status?2007
Author(s)
Crane, C, Akhter, N, Johnson, BW, Iruthayanathan, M, Syed, F, Kudo, A, Zhou, YH, Childs, GV
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Journal Title
Journal of Histochemistry and Cytochemistry 55
Pages: 1059-1073
Description
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[Journal Article] The effect of dexamethasone on defective nephrin transport caused by ER stress : a potential mechanism for the therapeutic action of glucocorticoids in the acquired glomerular diseases2006
Author(s)
Fujii, Y, Khoshnoodi, J, Takenaka, H, Hosoyamada, M, Nakajo, A, Bessho, F, Kudo, A, Takahashi, S, Arimura, Y, Yamada, A, Nagasawa, T, Ruotsalainen, V, Tryggvason, K, Lee, AS, Yan, K
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Journal Title
Kidney International 69
Pages: 1350-1359
Description
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[Journal Article] Localization of NBC1 Variants in Rat Kidney2006
Author(s)
Endo, Y, Yamazaki, S, Moriyama, N, Li, Y, Ariizumi, T, Kudo, A, Kawakami, H, Tanaka, Y, Horita, S, Yamada, H, Seki, G, Fujita, T
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Journal Title
Nephron Physiology 104
Pages: 87-94
Description
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[Presentation] Expression of endothelin receptor in sympathetic nerve terminals of guinea pig heart. (in Japanese)2007
Author(s)
Kudo, A, Matsubara, S, Isaka, M, Imamura, M, Yasuda, K, Kawakami, H
Organizer
112th Annual Meeting of the Japanese Association of Anatomists
Place of Presentation
Osaka
Description
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[Presentation] Cloning and analysis of three carbohydrate recognition domain(CRD)-type MGLs expressed in germ cells2006
Author(s)
Morikawa, A, Kudo, A, Kuroda, M, Tsuiji, M, Higashi, N, Kawakami, H, Irimura, T
Organizer
20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
Place of Presentation
Kyoto
Description
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[Presentation] Expression of matrix metalloproteinase(MMP)-2, -9, tissue inhibitor of metalloproteinase(TIMP)-1, -2 in human glomerular diseases. (in Japanese)2005
Author(s)
Sekiuchi, M, Kudo, A, Kanai, M, Akimoto, Y, Nakabayashi, K, Yamada, A, Kawakami, H
Organizer
61st Annual Meeting of the Japanese Society of Microscopy
Place of Presentation
Tsukuba
Description
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